Periodic Enzyme Synthesis in Synchronous Cultures of Yeast

Halvorson, Harlyn O.; Bock, Robert M.; Tauro, P.; Epstein, R.; Berge, M. La

doi:10.1016/b978-1-4832-2925-6.50011-5

Cited by 26 publications

(19 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In bacteria, although basal enzyme synthesis is ordered, induced enzyme synthesis may occur at any stage of the cell cycle (Masters et al 1964;Kuempel et al 1965;Masters & Donachie, 1966). In contrast, induced enzyme synthesis in yeasts shows a marked periodicity which, it has been suggested, may be due to variations in the accessibility of genes to transcription during the cell cycle (Halvorson et al 1966). Thus it follows that, in yeasts, the synthesis of catalase will be subject to this periodic inducibility.…”

Section: Discussionmentioning

confidence: 77%

“…It was suggested that only cells in which there is an active synthesis of catalase at the time of pressure increase survive: in such cells a high catalase level will be maintained throughout the cell cycle since, in bacteria, induced enzyme synthesis can occur throughout the cycle (Masters, Kuempel & Pardee, 1964;Kuempel, Masters & Pardee, 1965;Masters & Donachie, 1966). This paper describes the effect of high pressure oxygen (HPO) on cultures of Saccharomyces cerevisiae, an organism known to display periodic enzyme synthesis (Halvorson et al 1966) and on Candida utilis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Toxicity of Hyperbaric Oxygen to Yeasts Displaying Periodic Enzyme Synthesis

Gifford

Pritchard

1969

Journal of General Microbiology

View full text Add to dashboard Cite

SUMMARYExponential cultures of Saccharomyces cerevisiae and Candida utilis exposed to oxygen at ten atmospheres did not undergo any further cell division during treatment. All cells ultimately died when the treatment was prolanged for several days. Populations survived considerably longer with ethanol as a carbon source than with glucose. Stationary-phase populations were more resistant than exponentially-growing populations. The cell cycle of S. cerevisiae shows two points of resistance to oxygen toxicity which corresponded to two periods of catalase synthesis in air. These results are attributed to periodic synthesis of protective enzymes.

show abstract

Section: Discussionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Toxicity of Hyperbaric Oxygen to Yeasts Displaying Periodic Enzyme Synthesis

Gifford

Pritchard

1969

Journal of General Microbiology

View full text Add to dashboard Cite

show abstract

“…One characteristic method that has been used to obtain such synchronous cultures involves alternate rounds of nutritional starvation and recovery (17,52 (2).…”

Section: Discussionmentioning

confidence: 99%

“…Among the enzymes studied was aglucosidase (17,18,21,46,48). It was found that with induced and uninduced conditions the activity of the enzyme was consistently twofold higher in cells extracted at a particular period after division and remained elevated until the next division.…”

Section: Discussionmentioning

confidence: 99%

“…The data suggested that over 30 enzymes, including the catabolic enzyme a-glucosidase, showed characteristic twofold jumps in activity with time in synchronized cultures (19,21,38,39,48). However, such data faceC a degree of criticism, in that as the cells were synchronized by induction (alternating cycles of nutritional starvation and release) or by selection (zonal centrifugation in a sucrose density gradient), there was chance for artifact (17,46,52). Furthermore, measurements were exclusively of enzyme activity and not specific protein synthesis; thus, the level of control was unclear.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Synthesis of specific identified, phosphorylated, heat shock, and heat stroke proteins through the cell cycle of Saccharomyces cerevisiae.

Ludwig¹,

Foy²,

Elliott³

et al. 1982

Mol. Cell. Biol.

View full text Add to dashboard Cite

The methods of centrifugal elutriation, two-dimensional gel electrophoresis, and dual isotopic labeling were applied to the study and identification of a number of purified yeast proteins. The location of polypeptide spots corresponding to specific proteins was determined on two-dimensional gels. A dual-label method was used to determine the rates of synthesis through the cell cycle of the identified proteins as well as to confirm the results of previous studies from our laboratory on unidentified proteins. The identified proteins, and the more generally defined phosphorylated, heat shock, and heat stroke proteins were found to follow the general pattern of exponential increase in rate of synthesis through the cell cycle. In addition, colorimetric enzyme activity assays were used to examine the catabolic enzyme ca-glucosidase (EC 3.2.1.20). Both the activity and synthesis of a-glucosidase were found to be nonperiodic with respect to the cell cycle. These data contrast with earlier reports of periodicity, which employed induction and selection synchrony to study enzyme expression through the yeast cell cycle.The cell cycles of eucaryotic organisms contain a number of distinct events which take place only once in each cell cycle and are restricted to a defined period. In the budding yeast Saccharomyces cerevisiae, such distinct, one-time events include: spindle pole body duplication and separation, bud emergence, DNA synthesis, nuclear migration, nuclear division, cytokinesis, and cell separation (23). In addition to distinct events, cells undergo an exponential increase in size through the cell cycle (23). In light of the temporal sequence of events through the cell cycle, one might expect to see temporal expression of specific protein products responsible for (or dependent upon) such events.Workers in this laboratory have approached the problem by applying the technique of centrifugal elutriation to the separation of asynchronous, exponentially growing populations of yeast cells into cell cycle specific fractions (14). During elutriation, cells in the separation chamber are acted upon by a centrifugal force which tends to sediment them. This force is opposed by a flow of liquid, and with increasing flow rate, cells of increasing size are washed from the chamber and collected into specific fractions. t Current address:

show abstract

The Cell Cycle in Yeast-a Review

Duffus

1971

Journal of the Institute of Brewing

View full text Add to dashboard Cite

show abstract

Periodic Enzyme Synthesis in Synchronous Cultures of Yeast

Cited by 26 publications

References 22 publications

Toxicity of Hyperbaric Oxygen to Yeasts Displaying Periodic Enzyme Synthesis

Toxicity of Hyperbaric Oxygen to Yeasts Displaying Periodic Enzyme Synthesis

Synthesis of specific identified, phosphorylated, heat shock, and heat stroke proteins through the cell cycle of Saccharomyces cerevisiae.

The Cell Cycle in Yeast-a Review

Contact Info

Product

Resources

About