Periodic fluctuations in proliferation of SV-40 transformed human skin fibroblast lines with prolonged lifespan

Wolfrom, C.; Raynaud, Nicole; Maigne, J.; Papathanassiou, S.; Conti, Marc; Kadhom, Noman; Hecquet, B; Lévi, Françis; Gautier, M; Deschatrette, Jean

doi:10.1007/bf00756764

Cited by 11 publications

(8 citation statements)

References 23 publications

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“…Proliferation curves of both normal and transformed human embryonic fibroblasts, and of Fao hepatoma cells, maintained in longterm culture, showed regular rises and falls of cell proliferation rate. These observations are similar to those previously reported for SV40T-transformed human skin fibroblasts (34). These waves of cell proliferation were about 3 to 4 wk long, with variations depending on the cell line, for cells passaged about every week.…”

Section: Growth Of Rat Hepatoma Fao Cellssupporting

confidence: 88%

“…However, we previously showed that the rate of proliferation of such cell lines, whether long established like hamster Nil cells, or human fibroblasts with life span extended by recent SV40-T transformation, is not constant. The proliferation pattern displays regular rises and fails (34). Here, we report the population dynamics of three other long-lived cell lines: the rat hepatoma Fao cell line and one embryonic human fibroblast cell line and its ~Equal contributors to these studies.…”

Section: Introductionmentioning

confidence: 95%

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The time-pattern of rises and falls in proliferation fades with senescence of mortal lines and is perpetuated in immortal rat hepatoma fao cell line

Maigne

Deschatrette

Sarrazin

et al. 1998

In Vitro Cell.Dev.Biol.-Animal

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Immortal cells perpetuate the rises and falls of proliferation that are progressively damped in mortal long-term cultured cells. For immortal rat hepatoma Fao cells, similar waves of proliferation occurred about every 3-4 wk. Under the same conditions, embryonic human fibroblasts and transformed but not immortalized embryonic fibroblasts display similarly recurring proliferation waves that progressively decrease in amplitude until senescence of the lines. In addition, strains of diploid normal human skin fibroblasts cultured under different culture conditions display a similar time-pattern of proliferation. Although the amplitude and baseline of these fluctuations are characteristic for each cell line, a common point was marked slow down in proliferation after every sequence of about 25 population doublings for all cells. Renewed proliferation waves of Fao cells allow about 22-23 additional population doublings each. Normal embryonic fibroblast culture and its transformed counterpart accumulate about 30 and 60 population doublings, respectively, before senescence. Normal fibroblast strains accumulate about 25 population doublings over their entire life spans. This halt in proliferation after every stretch of about 25 population doublings may correspond to a structural or functional stop following attrition of telomeric DNA. This putative stop may be bypassed once in transformed embryonic cells and repetitively in immortal cells. In support of this hypothesis, we observed rapid telomere shortening, in two steps, during divisions of mortal embryonic cells, and maintenance of long telomeres in immortal Fao cells, which may indicate episodic repair of telomeres. Alternatively, such maintenance of long telomeres may reflect survival and successive clonal growth of rare cells with long telomeres. We suggest that the balance between telomere attrition and repair processes regulates the waves of proliferation.

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Section: Growth Of Rat Hepatoma Fao Cellssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 95%

The time-pattern of rises and falls in proliferation fades with senescence of mortal lines and is perpetuated in immortal rat hepatoma fao cell line

Maigne

Deschatrette

Sarrazin

et al. 1998

In Vitro Cell.Dev.Biol.-Animal

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“…1 and 2 with the highest deuterium content D/H 500.000 ppm and D/H 100.000 ppm. Since the PDT serves as one of the main cell proliferation activity indicators [ 20 ], its increase in the ADSCs population indicates cell cycle slowdown and, as a consequence, a decrease in the cell proliferation rate at high deuterium levels in the growth medium.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells

Zlatska

Gordiienko

Vasyliev

et al. 2018

The Scientific World Journal

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In current in vitro study we have shown the impact of deuterium content in growth medium on proliferation rate of human cultured adipose-derived stem cells (ADSC). ADSCs have also demonstrated morphological changes when cultured in deuterated growth medium: the cell cultures did not reach confluence but acquired polygonal morphology with pronounced stress fibers. At high deuterium concentrations the ADSCs population doubling time increased which indicated the cell cycle retardation and decrease of cell proliferation rate. The deuterated and deuterium-depleted growth media demonstrated acute and chronic cytotoxicity, respectively. The minimal migration ability was observed in deuterated medium whereas the highest migration activity was observed in the medium with the deuterium content close to natural. The cells in deuterated growth medium demonstrated decrease in metabolic activity after three days in culture. In contrast, in deuterium-depleted medium there was an increase in ADSC metabolic activity.

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“…SigmaScan Pro 5.0 Analysis Software (SPSS Inc., USA) was used to count cells on images. The proliferation rate of ASCs was estimated as the number of population doublings (PD), PD = log 2 N/No, where No and N—initial and final cell number …”

Section: Methodsmentioning

confidence: 99%

Proinflammatory interleukins' production by adipose tissue‐derived mesenchymal stromal cells: the impact of cell culture conditions and cell‐to‐cell interaction

Andreeva

Андрианова

Rylova

et al. 2015

Cell Biochemistry & Function

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The impact of culture conditions and interaction with activated peripheral blood mononuclear cells on the interleukin (IL) gene expression profile and proinflammatory IL-6 and IL-8 production by adipose-derived stromal cells (ASCs) was investigated. A microarray analysis revealed a wide range of IL genes either under standard (20%) or hypoxic (5%) O2 concentrations, some highly up-regulated at hypoxia. IL-6 and IL-8 production was inversely dependent on cell culture density. In early (first-third) passages, IL-6 and IL-8 concentration was higher at 20% O2 and in late (8th-12th) passages under 5% O2. Interaction between ASCs and mononuclear cells in indirect setting was accompanied with a significant decrease of IL-6 and did not result in the elevation of IL-8 concentration. Thereby, the production of proinflammatory interleukins (IL-6 and IL-8) may be affected by the ASC intrinsic features (density in culture, and duration of expansion), as well as by microenvironmental factors, such as hypoxia and the presence of blood-borne cells. These data are important for elucidating ASC paracrine activity regulation in vitro. They would also be on demand for optimisation of the cell therapy protocols, based on the application of ASC biologically active substances. SIGNIFICANCE PARAGRAPH: Ex vivo expansion is widely used for increasing the number of adipose-derived stromal cells (ASCs) and improving of their quality. The present study was designed to elucidate the particular factors influencing the interleukin production in ASCs. The presented data specified the parameters (i.e. cell density, duration of cultivation, hypoxia, etc.) that should be taken in mind when ASCs are intended to be used in protocols implying their paracrine activity. These data would be of considerable interest for researchers and clinicians working in the biomedical science.

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Periodic fluctuations in proliferation of SV-40 transformed human skin fibroblast lines with prolonged lifespan

Cited by 11 publications

References 23 publications

The time-pattern of rises and falls in proliferation fades with senescence of mortal lines and is perpetuated in immortal rat hepatoma fao cell line

The time-pattern of rises and falls in proliferation fades with senescence of mortal lines and is perpetuated in immortal rat hepatoma fao cell line

In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells

Proinflammatory interleukins' production by adipose tissue‐derived mesenchymal stromal cells: the impact of cell culture conditions and cell‐to‐cell interaction

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