1994
DOI: 10.1021/ma00099a019
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Periodic Motion of Large DNA Molecules during Steady Field Gel Electrophoresis

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Cited by 60 publications
(86 citation statements)
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“…A chain moving in a relatively collapsed form (a) is trapped by a certain obstacle (b), deforms to a V-or U-shaped conformation (c,d), slides off the obstacle (e), and then tends to form a collapsed conformation again (f). In contrast to experimental observation [13], however, quasi-periodical alternation between contracted and extended conformations has not yet been observed, or periods between the two conformations are rather random.…”
Section: Resultscontrasting
confidence: 94%
See 1 more Smart Citation
“…A chain moving in a relatively collapsed form (a) is trapped by a certain obstacle (b), deforms to a V-or U-shaped conformation (c,d), slides off the obstacle (e), and then tends to form a collapsed conformation again (f). In contrast to experimental observation [13], however, quasi-periodical alternation between contracted and extended conformations has not yet been observed, or periods between the two conformations are rather random.…”
Section: Resultscontrasting
confidence: 94%
“…Also experimentally, since fluorescent microscopy has been invented and improved, evidences showing this instability have increased as well, and now details of DNA motion itself are of current interest in the study of gel electrophoresis [9,10,11,12]. Among them the inch-worm like motion may be the most typical example which is observed for large DNA [13].…”
Section: Introductionmentioning
confidence: 99%
“…They found that YOYO-stained T2 DNA molecules in 1% agarose gel at 8 V/cm in average spend 68% of the oscillation time in the U-formation/deformation step, 22 Yo in the I-contraction step, and 10% as compressed conformations. These data are consistent with the observation that the velocity of the tail end of the DNA molecule during the I-contraction step is about four times higher than the velocity of the front part (which is constant during the cycle), i.e., the molecule spends, on average, three times more time in the U-formation/deformation step than in the I-contraction step [22]. According to Eq.…”
Section: Simulationssupporting
confidence: 90%
“…By optimally shaping the field pulses, the molecular reorientation time can be cut in half (compared with constant-height pulses), reducing proportionally the separation time and still avoiding trapping. Assume that the pulse has been reversed for a time t and that the arms extend (from zero length) at the rate E, where is the mobility of the DNA (4,29). The force builds in time according to F ϭ eff E 2 t until the molecular contour length, L, is reached, and then F Ͻ eff EL͞2.…”
Section: Discussionmentioning
confidence: 99%