Sixteen cows in middle to late lactation were milked for 3.5 days at 12-h intervals except for a 24-h interval between third and fourth milkings. A cowside quarter milking unit was used. Quarters were classified by infection status. Milk chloride, lactose, somatic cell concentrations, N-acetyl-B-D-glucosaminidase activity, and cell differential counts were determined. Following the omitted milking, concentrations of milk chloride and somatic cells were elevated and lactose concentration reduced in infected quarters. In uninfected quarters, chloride concentration increased, and lactose concentration decreased after the 24-h interval. The milk N-acetyl-B-D-glucosaminidase activity was elevated only in quarters infected with major pathogens. Changes of milk secretion induced by an omitted milking are affected by infection status, and additional secretory cell damage in quarters infected with a major pathogen may result from an omitted milking.