Periodontal Healing of Replanted Monkey Teeth Prevented from Drying

Blomlöf, Leif; Andersson, Lars; Lindskog, Sven; Hedström, Karl‐Goran; Hammarström, Lars

doi:10.3109/00016358309162311

Cited by 84 publications

(106 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For up to an hour the vitality of the cement can be maintained by wrapping the tooth in plastic foil. 3 For longer than that cell viability can be sustained by storing the tooth in milk, which has an osmolarity and pH (230-270 mmol/kg and 6 6) compatible with cell survival. 4 Reimplantation is carried out by rinsing the tooth gently in saline and placing it in the socket, taking care to handle it by the crown.…”

Section: Emergency Treatment Of Avulsed Incisor Teethmentioning

confidence: 99%

Emergency treatment of avulsed incisor teeth.

Scheer¹

1990

BMJ

View full text Add to dashboard Cite

Section: Emergency Treatment Of Avulsed Incisor Teethmentioning

confidence: 99%

Emergency treatment of avulsed incisor teeth.

Scheer¹

1990

BMJ

View full text Add to dashboard Cite

“…Posteriormente, el conducto fue obturado con un apósito de hidróxido de calcio en pasta, Calcifar -P (Eufar ® ) (Figura 2 y 3). [21][22][23][24][25][26][27][28][29][30][31][32][33] Figura 2. Estimación de la longitud de trabajo en 20 mm.…”

Section: Tratamiento Del Conducto Radicularunclassified

Tratamiento De Una Reabsorción Externa Inflamatoria Con Trióxido De Mineral Agregado: Reporte De Un Caso

Arquez

2009

UstaSalud

View full text Add to dashboard Cite

RESUMENPaciente masculino de 9 años de edad, sin antecedentes médicos que contribuyan al caso. En el examen clínico y radiográfico se observó una extensa y progresiva reabsorción radicular externa inflamatoria, una extensa lesión periapical, una bolsa periodontal y movilidad dentaria grado 2 a nivel del 21. Cinco meses atrás, el paciente sufrió trauma dental (avulsión), ese incisivo, fue mantenido en condiciones secas (envuelto en un pañuelo), reimplantado, reposicionado y ferulizado por odontólogo general dos horas después del trauma. Seguido a la preparación biomecánica se realizaron recambios de hidróxido de calcio cada cuatro semanas durante 6 meses; las características clínico patológicas mostraron una resolución parcial del proceso (disminución de la lesión periapical, pero persistía la pérdida ósea, la reabsorción radicular externa, la movilidad dentaria grado 2; y el defecto periodontal). Se decidió obturar completamente el conducto radicular con MTA blanco, los exámenes radiográficos seis meses después mostraron cambios en la densidad -trabeculado del hueso alveolar y la lámina ósea alrededor de la superficie radicular, no fue encontrada bolsa periodontal, la movilidad del diente fue considerada normal (grado 1), con subsecuente detención del proceso de reabsorción. Radiografías a los 24-48-54 meses mostraron una adecuada reparación de la reabsorción. Clínicamente el diente fue asintomático. El propósito de este artículo es reportar la reparación y detención de una extensa reabsorción radicular externa inflamatoria mediante el uso de MTA. TREATMENT OF AN EXTERNAL INFLAMMATORY RESORPTION WITH MINERAL TRIOXIDE AGGREGATE: A CASE REPORT ABSTRACTA 9-year old male, the patient`s medical histories were all noncontributory. A clinical and radiographic examination revealed an extensive and progressive external inflamatory, extensive periapical lesion, periodontal pocket, grade II mobility at level of the 21 Five months behind the patient suffered dental trauma (avulsion) , that incisive, was stored under dry conditions (wrapped in a handkerchief), replanted ,repositioned and splinted by general dentist 2 hours after trauma. Following biomechanical preparation, a dressing of calcium hydroxide was applied and replaced every 4 weeks over a period of 6 months; The clinical-pathological picture demonstrated partial resolution (decrease of periapical lesion, but it persisted: the loss of bony, the external root resorption, the grade II mobility, and the defect periodontal) decides to filling the root canal completely with MTA white, The radiographic examinations 6 mouth after revealed changes in the density -trabeculade of the alveolar bone, and the lamina bony around the root surface, no periodontal pocket was found, the mobility tooth was considered normal (grade I), with subsequent arrest of the process resorptive. Radiographs at 24, 48, 54 months showed adequate repair of the resorption. Clinically, the tooth was asymptomatic. The aim of this article is report the repair and arrest of an extensive external inflam...

show abstract

“…Blomlof et al (1983) Pre-Replantation Contamination Lindskog and Blomlof (1982) noted the adherence of bacteria to the surface membrane of PL cells stored in saliva. This is important because of the lytic effect of bacterial enzymes and toxins (Lindskog and Blomlof 1982 …”

Section: Pre-replantation Duration and Storaaementioning

confidence: 99%

“…After 120 minutes of desiccation, no viable cells remain on the root surface (Soder et al 1977; Lekic et al 1998). A histological outcome study of lateral incisors from 8 monkeys suggested that 30-40 minutes rnay be the b i t of drying to avoid severe root resorption as early as 8 weeks after replantation (Blomlof et al 1983). …”

Section: Pre-replantation Duration and Storaaementioning

confidence: 99%

Storage conditions of avulsed teeth affect the phenotype of cultured human periodontal ligament cells

Lin

Kenny

Barrett

et al. 2000

J of Periodontal Research

View full text Add to dashboard Cite

After severe injury to the periodontal ligament (PL), the phenotypes of cells recolonizing root surfaces influence the extent and type of repair processes. In teeth that are replanted following avulsion injury, recolonization of the PL space by osteogenic cells instead of by PL fibroblasts may favor bone formation (i.e. ankylosis) instead of PL regeneration. We consider here that recolonization processes depend in part on the storage conditions of the teeth following avulsion. We used an in vitro cell culture model to assess the effect of storage conditions on immunohistochemical staining of several marker proteins that are expressed by osteogenic cells (osteopontin and alkaline phosphatase) and fibroblasts (α‐smooth muscle actin, type III and XII collagens). Prior to cell culture, extracted human premolar teeth were stored in air (“dry”) or in α‐MEM (“wet”) for either 30 or 120 min as surrogate conditions for the variations of extra‐alveolar tooth storage that may occur following avulsion. Collagenase/trypsin‐digested suspensions of PL cells were prepared from the tissue adherent to the extracted root surface. Passage #2 or #3 cultures were immunostained and examined by fluorescence microscopy. For type XII collagen, cells from wet samples displayed perinuclear staining while cells from 30‐min dry samples showed only isolated foci. The staining for 120‐min dry samples was weak and non‐specific. α‐Smooth muscle actin was not incorporated into stress fibers in wet samples, whereas dry samples demonstrated prominent stress fibers stained for α‐smooth muscle actin. Detached cytoplasmic fragments resembling cell processes that stained for α‐smooth muscle actin were abundant in dry samples, indicating the presence of highly contractile cells. The staining for osteopontin was mainly perinuclear but was more intense in dry samples. The focal adhesion pattern of osteopontin staining in 120‐min dry samples resembled that of migrating osteogenic cells. The pattern of staining did not vary for type III collagen or alkaline phosphatase, although staining for alkaline phosphatase was more intense in samples stored under dry conditions. We conclude that prolonged extra‐alveolar dry storage favors increased in vitro growth of contractile cells expressing osteogenic cell markers while storage in cell culture medium favors growth of cells with the classical phenotype of PL fibroblasts.

show abstract

Periodontal Healing of Replanted Monkey Teeth Prevented from Drying

Cited by 84 publications

References 12 publications

Emergency treatment of avulsed incisor teeth.

Emergency treatment of avulsed incisor teeth.

Tratamiento De Una Reabsorción Externa Inflamatoria Con Trióxido De Mineral Agregado: Reporte De Un Caso

Storage conditions of avulsed teeth affect the phenotype of cultured human periodontal ligament cells

Contact Info

Product

Resources

About