Periodontal healing with a preameloblast‐conditioned medium in dogs

Yu, Sang Joun; Lee, D.-S.; Kim, B.-O.; Choi, Seong‐Ho; Park, J.-C.

doi:10.1111/jre.12307

Cited by 6 publications

(3 citation statements)

References 41 publications

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“…Epithelial rests of Malassez in the periodontal ligament (PDL) also protect against resorption ( Keinan & Cohen, 2013). Periodontal research in dogs has shown that signalling molecules can significantly repair experimentally induced replacement resorption after damage to the cementum layer where it is hypothesized PDL cells differentiate into cementoblasts (Yu et al, 2016). It is therefore plausible that that signalling molecules and cytokines from REPs can affect not only the regeneration of pulp-like tissue but also enhanced mineralization and regeneration/repair of the roots affected with ERR.…”

Section: Toothmentioning

confidence: 99%

Clinical and radiographic outcomes of regenerative endodontic procedures for traumatized permanent necrotic teeth with apical periodontitis and external root resorption

Lü

et al. 2023

Int Endodontic J

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Aim: This clinical study was undertaken to assess the effect of treatment with regenerative endodontic procedures (REPs) on 20 teeth with pulp necrosis, apical periodontitis, and external root resorption (ERR).Methodology: Teeth were treated with REPS utilizing the American Association of Endodontists (AAE) protocol. Quantitative assessment of changes in radiographic root area (RRA) were statistically analysed to assess changes in root dimensions after an average follow-up period of 3 years.Results: All 20 teeth survived, 14 teeth (70%) were classified successful, and only 1 tooth (5%) failed throughout the study period. Based on the radiographic examination, all 20 teeth showed complete repair of the periapical lesions and arrested ERR. However, 5 teeth (25%) subsequently developed replacement resorption. The RRA between baseline and 3-year follow-up showed a significant difference for the total 20 teeth (p = .009). An analysis according to the trauma type and the extraoral time showed the RRA increase was significantly different in the non-avulsion group (p = .015) and for the avulsion group with an extra-oral time less than 60 min (p = .029). The RRA increase was not statistically significant in the avulsion group of extra-oral time more than 60 min (p = .405). Nine teeth (45%) and 10 teeth (50%) responded to cold and electric pulp testing, respectively. Conclusions:Within the limitations of this study, the favourable outcomes of REPs were further confirmed for traumatized permanent necrotic teeth with ERR in terms of periapical lesion healed and a significant increase in RRA. The study contributes further evidence of the role of REPs in arresting ERR.

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Section: Toothmentioning

confidence: 99%

Clinical and radiographic outcomes of regenerative endodontic procedures for traumatized permanent necrotic teeth with apical periodontitis and external root resorption

Lü

et al. 2023

Int Endodontic J

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“…In our previous studies, we aimed to regenerate the periodontium using dental epithelium‐derived factors. Preameloblast‐conditioned medium (PA‐CM) and Copine7 (CPNE7), a component of the PA‐CM, have been reported to regenerate the physiological structure of the PDL (Yu et al, 2016; Choung et al, 2019). CPNE7 treatment in three‐wall defect models resulted in rearranged PDL fibres in canines.…”

Section: Introductionmentioning

confidence: 99%

CPNE7 regenerates periodontal ligament via TAU‐mediated alignment and cementum attachment protein‐mediated attachment

Bai

Lee²,

Son

et al. 2022

J Clinic Periodontology

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Aim Once the periodontal ligament (PDL) is damaged, it is difficult to regenerate its characteristic structure. Copine7 (CPNE7) reportedly plays a functional role in supporting periodontal attachment and PDL alignment. Here we demonstrate the regulatory mechanism of CPNE7 coordination with cytoskeleton reorganization and cementum attachment protein (CAP)‐mediated attachment in PDL regeneration. Materials and Methods The expression and localization of CPNE7, α‐TUBULIN, ACTIN, and microtubule associated protein tau (TAU) were investigated in vitro. The effects of recombinant CPNE7 (rCPNE7) and CPNE7‐derived peptides (CPNE7‐DP) on the regulation of CAP were analysed in vitro, and PDL repair capacity was analysed in vivo. Results CPNE7 co‐localized with F‐ACTIN and induced α‐TUBULIN expansion to the edge of human PDL cells (hPDLCs). ACTIN and α‐TUBULIN protein expressions were not elevated in rCPNE7‐treated hPDLCs. rCPNE7 elevated the protein expression of TAU, which co‐localized with F‐ACTIN and α‐TUBULIN. Replantation studies on mice revealed that well‐attached and well‐aligned PDLs were repaired in the rCPNE7 group. CPNE7‐DP directly up‐regulate the expression of CAP in vitro and promote PDL regeneration in three‐wall defect canine models in vivo. Conclusions Our findings suggest that CPNE7 helps in PDL repair by supporting PDL alignment through TAU‐mediated cytoskeleton reorganization and direct regulation of CAP‐mediated PDL attachments of PDLCs.

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“…Esses achados sugerem que o selamento apical com tecido mineralizado tem uma orientação centrípeta, iniciando em regiões periféricas até o centro do forame, principalmente formado por cemento de tipo celular. A literatura que caracteriza a natureza do tecido mineralizado depositado no reparo apical após tratamento endodôntico é limitada, sendo o reparo do cemento mais estudado durante o tratamento ortodôntico (Mehta et al, 2017) e tratamento periodontal (Yu et al, 2016;Hernandez-Monjaraz et al, 2018).…”

Section: Discussionunclassified

Eficácia de um novo protocolo de tratamento endodôntico em sessão única. Estudo radiográfico e histopatológico em dentes de cães com lesões periapicais induzidas

Huamán¹

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Periodontal healing with a preameloblast‐conditioned medium in dogs

Abstract: These results suggest that PA-CM has the potential to regenerate periodontal tissues in PDL and cementum defects.

Cited by 6 publications

References 41 publications

Clinical and radiographic outcomes of regenerative endodontic procedures for traumatized permanent necrotic teeth with apical periodontitis and external root resorption

Clinical and radiographic outcomes of regenerative endodontic procedures for traumatized permanent necrotic teeth with apical periodontitis and external root resorption

CPNE7 regenerates periodontal ligament via TAU‐mediated alignment and cementum attachment protein‐mediated attachment

Eficácia de um novo protocolo de tratamento endodôntico em sessão única. Estudo radiográfico e histopatológico em dentes de cães com lesões periapicais induzidas

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