Periodontal ligament fibroblasts-derived exosomes induced by PGE2 inhibit human periodontal ligament stem cells osteogenic differentiation via activating miR-34c-5p/SATB2/ERK

Lin, Chen; Yang, Yingying; Wang, Yingxue; Jing, Heng; Bai, Xinyi; Zheng, Hong; Zhang, Chunxiang; Gao, Hui; Zhang, Linkun

doi:10.1016/j.yexcr.2022.113318

Cited by 11 publications

(7 citation statements)

References 38 publications

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“…The isolation and identification of hPDLCs-Exos were conducted following the protocols described in previous article 24 . Ultracentrifugation was employed for exosome isolation, while TEM, NTA and WB were utilized for exosome characterization.…”

Section: Resultsmentioning

confidence: 99%

“…Exosomes internalized by periodontal ligament stem cells have an impact on bone metabolism via releasing the contents effectively 26 . Previous research has shown that periodontal ligament fibroblasts-derived exosomes induced by Prostaglandin E 2 inhibit human periodontal ligament stem cells osteogenic differentiation 24 . In the pressure zone of OTM in rats, the expression of Interleukin-17 (IL-17) can be detected.…”

Section: Discussionmentioning

confidence: 99%

“…Our team will also continue to investigate whether transcription factors from different macrophage subtypes affect the osteoclastogenic potential. There are many factors that may affect the release of Exos from PDLCs, such as mechanical 36 , inflammatory 24 , and oxidative stress 47 conditions. It's crucial to explore methods that can accelerate the secretion of Exos from PDLCs.…”

Section: Discussionmentioning

confidence: 99%

“…The acquisition and cultivation of PDLCs, as well as the isolation and identification of hPDLCs-Exos, were conducted following the protocols described in previous article 24 . RAW264.7 (CL-0190) macrophages were kindly provided by Procell Life Science& Technology Co., Ltd.…”

Section: Methodsmentioning

confidence: 99%

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Periodontal ligament cells-derived exosomes promote osteoclast differentiation via modulating macrophage polarization

Bai,

Wang,

et al. 2024

Sci Rep

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Several studies have demonstrated that exosomes (Exos) are involved in the regulation of macrophage polarization and osteoclast differentiation. However, the characteristics as well as roles of exosomes from human periodontal ligament cells (hPDLCs-Exos) in M1/M2 macrophage polarization and osteoclast differentiation remain unclear. Here, periodontal ligament cells were successfully extracted by method of improved Type-I collagen enzyme digestion. hPDLCs-Exos were extracted by ultracentrifugation. hPDLCs-Exos were identified by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and western blotting (WB). Osteoclast differentiation was evaluated by real-time quantitative polymerase chain reaction (RT-qPCR), WB and tartrate-resistant acid phosphatase (TRAP) staining. M1/M2 macrophage polarization were evaluated by RT-qPCR and WB. The results showed hPDLCs-Exos promoted osteoclast differentiation and M2 macrophage polarization, but inhibited M1 macrophage polarization. Moreover, M1 macrophages inhibited osteoclast differentiation, whereas M2 macrophages promoted osteoclast differentiation. It has shown that hPDLCs-Exos promoted osteoclast differentiation by inhibiting M1 and promoting M2 macrophage polarization.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Periodontal ligament cells-derived exosomes promote osteoclast differentiation via modulating macrophage polarization

Bai,

Wang,

et al. 2024

Sci Rep

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“…[3,4,14]). Studies showed that sEVs derived from bone marrow-derived MSCs (BMSCs), [15][16][17] GM-SCs [18][19][20] and PDLCs [21][22][23][24][25] have demonstrated elevated osteogenesis and anti-inflammatory capability in the context of bone regeneration in vitro and in vivo. However, there have been few studies that have investigated the regenerative potential of EVs produced by alveolar bone-derived osteoblasts, as well as the comparison of the effects of hPDLCs-EVs, hGFs-EVs and hOBs-EVs on the differentiation fate of human buccal fat pad-mesenchymal stromal cells (hBFP-MSCs, a recently discovered source of multipotent MSCs within the oral cavity 26 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of periodontal cells‐derived extracellular vesicles on mesenchymal stromal cell function

Han,

Johnson,

Abdal‐hay

et al. 2023

J of Periodontal Research

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ObjectiveTo enrich and compare three extracellular vesicles—EV subtypes (apoptotic bodies, microvesicles and small EV) from three periodontal cells (periodontal ligament cells—PDLCs, alveolar bone‐derived osteoblasts—OBs and gingival fibroblasts—GFs), and assess uptake and cell function changes in buccal fat pad‐derived mesenchymal stromal cells (BFP‐MSCs).BackgroundPeriodontal cells such as PDLCs, OBs and GFs have the potential to enhance bone and periodontal regeneration, but face significant challenges, such as the regulatory and cost implications of in vitro cell culture and storage. To address these challenges, it is important to explore alternative ‘cell‐free’ strategies, such as extracellular vesicles which have emerged as promising tools in regenerative medicine, to facilitate osteogenic differentiation and bone regeneration.Methods and MaterialsSerial centrifuges at 2600 and 16 000 g were used to isolate apoptotic bodies and microvesicles respectively. Small EV—sEV was enriched by our in‐house size exclusion chromatography (SEC). The cellular uptake, proliferation, migration and osteogenic/adipogenic differentiation genes were analysed after EVs uptake in BFP‐MSCs.ResultsThree EV subtypes were enriched and characterised by morphology, particle size and EV‐associated protein expression—CD9. Cellular uptake of the three EVs subtypes was observed in BFP‐MSCs for up to 7 days. sEV from the three periodontal cells promoted proliferation, migration and osteogenic gene expression. hOBs‐sEV showed superior levels of osteogenesis markers compared to that hPDLCs‐sEV and hGFs‐sEV, while hOBs‐16k EV promoted adipogenic gene expression compared to that from hPDLCs and hGFs.ConclusionsOur proof‐of‐concept data demonstrate that hOBs‐sEV might be an alternative cell‐free therapeutic for bone tissue engineering.

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Non-coding RNAs Function in Periodontal Ligament Stem Cells

Zhai,

Gao,

Qin

et al. 2024

Stem Cell Rev and Rep

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Periodontal ligament fibroblasts-derived exosomes induced by PGE2 inhibit human periodontal ligament stem cells osteogenic differentiation via activating miR-34c-5p/SATB2/ERK

Cited by 11 publications

References 38 publications

Periodontal ligament cells-derived exosomes promote osteoclast differentiation via modulating macrophage polarization

Periodontal ligament cells-derived exosomes promote osteoclast differentiation via modulating macrophage polarization

Effects of periodontal cells‐derived extracellular vesicles on mesenchymal stromal cell function

Non-coding RNAs Function in Periodontal Ligament Stem Cells

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