Periodontal Ligament Stem Cells Possess the Characteristics of Pericytes

Iwasaki, Kengo; Komaki, Motohiro; Yokoyama, Naoki; Tanaka, Yuichi; Taki, Atsuko; Kimura, Yasuyuki; Takeda, Masaki; Oda, Shigeru; Izumi, Yuichi; Morita, Ikuo

doi:10.1902/jop.2012.120547

Cited by 70 publications

(60 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The percentages of IdU + cells at the peritenon, mid-substance, and tendonbone junction excluding the hypertropic chondrocyte zone were counted and the expression of Ki67, a cell proliferation marker, in IdU + cells at different regions was compared after co-immunofluorescent staining. The co-localization of LRCs (stable IdU + cells) with pericytes at the peritenon, mid-substance, and tendon-bone junction excluding the hypertropic chondrocyte zone was also examined by coimmunofluorescent staining with a pericyte-related marker (CD146) [20]. The co-localization of LRCs with CD44 and Sca-1, two commonly used in vitro MSC markers, in the midsubstance in intact tendon and at the window wound at day 7 after injury (see protocol below) was examined by coimmunofluorescent staining.…”

Section: Methodsmentioning

confidence: 99%

In Vivo Identity of Tendon Stem Cells and the Roles of Stem Cells in Tendon Healing

Tan

Lui

Lee

2013

Stem Cells and Development

View full text Add to dashboard Cite

We investigated the spatial distribution of stem cells in tendons and the roles of stem cells in early tendon repair. The relationship between tendon-derived stem cells (TDSCs) isolated in vitro and tendon stem cells in vivo was also explored. Iododeoxyuridine (IdU) label-retaining method was used for labeling stem cells in rat patellar tendons with and without injury. Co-localization of label-retaining cells (LRCs) with different markers was done by immunofluorescent staining. TDSCs were isolated from patellar tendon mid-substance after IdU pulsing, and the expression of different markers in fresh and expanded cells was done by immunofluorescent staining. More LRCs were found at the peritenon and tendon-bone junction compared with the mid-substance. Some LRCs at the peritenon were located at the perivascular niche. The LRC number and the expression of proliferative, tendon-related, pluripotency, and pericyte-related markers in LRCs in the window wound increased. Most of the freshly isolated TDSCs expressed IdU, and some TDSCs expressed pericyte-related markers, which were lost during expansion. Both freshly isolated and subcultured TDSCs expressed pluripotency markers, which were absent in LRCs in intact tendons. In conclusion, we identified LRCs at the peritenon, mid-substance, and tendon-bone junction. There were both vascular and non-vascular sources of LRCs at the peritenon, while the source of LRCs at the mid-substance was non-vascular. LRCs participated in tendon repair via migration, proliferation, activation for tenogenesis, and increased pluripotency. Some LRCs in the window wound were pericyte like. Most of the mid-substance TDSCs were LRCs. The pluripotency markers and pericyte-related marker in LRCs might be important for function after injury. Recently, stem/progenitor cells have been isolated from tendon tissues of varies species, including human, horse, rabbit, rat, and mouse [3][4][5][6]. These stem/progenitor cells isolated from tendon tissues exhibited self-renewal and multilineage differentiation potential [3][4][5][6]. Since the origin and identity of these cells were not clear, we called them tendon-derived stem cells (TDSCs) to indicate only the tissue from which the cells were isolated [5].Many studies suggested that the wall of capillaries, small vessels, and large vessels harbored stem/progenitor cells [7][8][9][10][11]. Some studies further suggested that mesenchymal stem cells (MSCs) were derived from pericytes [9,12].Pericytes/perivascular cells from a variety of tissues were reported to exhibit characteristics that were strikingly similar to those of MSCs [7][8][9][10][11]. For tendons, there was also evidence that the vasculature of tendon tissue might harbor stem cells [13]. However, tendon mid-substance is hypovascular compared with other tissues and receives its blood supply mainly from the endotenon and paratenon [14]. TDSCs isolated from the tendon mid-substance, while positive for alpha smooth muscle actin [5], were not positive for other pericyte-related markers [3,15]. Tend...

show abstract

Section: Methodsmentioning

confidence: 99%

In Vivo Identity of Tendon Stem Cells and the Roles of Stem Cells in Tendon Healing

Tan

Lui

Lee

2013

Stem Cells and Development

View full text Add to dashboard Cite

show abstract

“…33 Moreover, we observed that 32.9% hPDLSCs expressed CD146, which can be taken to interpret that PDLSCs located in the perivascular wall of periodontal ligaments and express pericyte-specific markers CD146. 34 MSCs derived from some other tissues (bone marrow, skin, liver, lung) also express CD146. 35 More importantly, PDLSCs exhibited the abilities to form bone/cementum-like mineralized tissue and ligament structures similar to Sharpey's fibers with an associated vasculature.…”

mentioning

confidence: 99%

Effect of micro-nano-hybrid structured hydroxyapatite bioceramics on osteogenic and cementogenic differentiation of human periodontal ligament stem cell via Wnt signaling pathway

Mao¹,

Liu²,

Zhao³

et al. 2015

IJN

View full text Add to dashboard Cite

The surface structure of bioceramic scaffolds is crucial for its bioactivity and osteoinductive ability, and in recent years, human periodontal ligament stem cells have been certified to possess high osteogenic and cementogenic differential ability. In the present study, hydroxyapatite (HA) bioceramics with micro-nano-hybrid surface (mnHA [the hybrid of nanorods and microrods]) were fabricated via hydrothermal reaction of the α-tricalcium phosphate granules as precursors in aqueous solution, and the effects of mnHA on the attachment, proliferation, osteogenic and cementogenic differentiations of human periodontal ligament stem cells as well as the related mechanisms were systematically investigated. The results showed that mnHA bioceramics could promote cell adhesion, proliferation, alkaline phosphatase (ALP) activity, and expression of osteogenic/cementogenic-related markers including runt-related transcription factor 2 (Runx2), ALP, osteocalcin (OCN), cementum attachment protein (CAP), and cementum protein (CEMP) as compared to the HA bioceramics with flat and dense surface. Moreover, mnHA bioceramics stimulated gene expression of low-density lipoprotein receptor-related protein 5 ( LRP5 ) and β-catenin , which are the key genes of canonical Wnt signaling. Moreover, the stimulatory effect on ALP activity and osteogenic and cementogenic gene expression, including that of ALP, OCN, CAP, CEMP, and Runx2 of mnHA bioceramics could be repressed by canonical Wnt signaling inhibitor dickkopf1 (Dkk1). The results suggested that the HA bioceramics with mnHA could act as promising grafts for periodontal tissue regeneration.

show abstract

“…NG2-expressing PCs per se serve as blood vessel progenitor cells [108,109] and can generate cardiomyocytes, smooth muscles and vascular EC in homeostatic conditions and after myocardial injuries for constitution of myocardium within the adult heart. Isolation and stimulation of PCs in adult heart may represent a potential therapeutic strategy to treat heart failures [110][111][112].…”

Section: Adult Cardiovascular Ng2 + Cells In Heart Failure Repairmentioning

confidence: 99%

Adult NG2-Expressing Cells in Multiple Organs: A Novel Progenitor in Regenerative Medicine

H¹

2013

J Genet Syndr Gene Ther

View full text Add to dashboard Cite

Periodontal Ligament Stem Cells Possess the Characteristics of Pericytes

Abstract: PDLSCs possess pericyte-like characteristics and may localize as pericytes in the PDL. These data provide useful information for stem cell biology in periodontal research and stem cell-based periodontal therapy.

Cited by 70 publications

References 33 publications

In Vivo Identity of Tendon Stem Cells and the Roles of Stem Cells in Tendon Healing

In Vivo Identity of Tendon Stem Cells and the Roles of Stem Cells in Tendon Healing

Effect of micro-nano-hybrid structured hydroxyapatite bioceramics on osteogenic and cementogenic differentiation of human periodontal ligament stem cell via Wnt signaling pathway

Adult NG2-Expressing Cells in Multiple Organs: A Novel Progenitor in Regenerative Medicine

Contact Info

Product

Resources

About