Periodontal Regeneration by Allogeneic Transplantation of Adipose Tissue Derived Multi-Lineage Progenitor Stem Cells in vivo

Venkataiah, Venkata Suresh; Handa, Keisuke; Njuguna, Mary M.; Hasegawa, Tatsuya; Maruyama, Kentaro; Nemoto, Eiji; Yamada, Satoru; Sugawara, Shunji; Lu, Lu; Takedachi, Masahide; Murakami, Shinya; Okura, Hanayuki; Matsuyama, Akifumi; Saito, Masahiro

doi:10.1038/s41598-018-37528-0

Cited by 46 publications

(53 citation statements)

References 47 publications

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“…As we know, ASCs have been applied in periodontal regeneration, bone regeneration and wound repair [31][32][33]. In this study, the proliferation rate and migration speed of Peri-ASCs significantly increased compared with Con-ASCs.…”

Section: Discussionmentioning

confidence: 71%

Effects of experimental periodontitis on proliferation and osteogenic differentiation of adipose-derived stem cells in rats

Huang

Cheng

Fan

et al. 2019

Preprint

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KEYWORDSEffects of experimental periodontitis, proliferation and osteogenic differentiation of adipose-derived stem 2 Abstract Background and Objective: The application of ASCs in periodontal regeneration is a good choice.Inflammatory micro-environment influenced the proliferation, mobilization, and osteogenic differentiation of ASCs in vitro.The aim of this study was to evaluate the effects of experiment periodontitis on the proliferation, wound healing and osteogenesis markers of adipose-derived stem cells (ASCs) in rats.Materials and methods: Ten male rats were divided into two groups randomly. The control (Con) group received a standard diet, and the periodontitis (Peri) group was received a standard diet with placing ligatures around the maxillary first molar. Toll like receptor 4 (TLR4), Tumor necrosis factor-α (TNF-a) and Interleukin-1β (IL-1β) were tested by immunohistochemistry (IHC) staining and quantitative real-time polymerase chain reaction (qRT-PCR). The proliferation rate of ASCs was measured through Cell Counting Kit-8 (CCK-8) assay. The migration speed of stem cells was evaluated by using a wound healing assay. The expression of alkaline phosphatase (ALP), bone morphogenetic protein-2 (BMP2) and runt related transcription factor 2 (Runx2) was evaluated by qRT-PCR analysis and western blot. Graph Pad Primer 7.0 software was used for statistical analysis. Results: After 4 weeks, periodontitis model was successfully constructed. The results of IHC and RT-PCR found that in the Peri group, the TNF-a and IL-1β levels of adipose tissues decreased compared with the Con group (P<0.05). The proliferation of Peri-ASCs significantly increased compared withCon-ASCs. Moreover, the wound healing ability of Peri-ASCs gradually increased in a time dependent manner compared with Con-ASCs. Results of RT-PCR showed that ALP and BMP2 gene levels of Peri-ASCs significantly decreased (P<0.05), while the Runx2 gene level in Peri-ASCs was increased, when compared to Con-ASCs. The ALP activity of Peri-ASCs was decreased compared to the Con-ASCs, especially the difference was significant at day 5 day (P<0.01). Western blot results showed that ALP, Runx2 and BMP2 protein levels of Peri-ASCs were significantly lower than those in Con-ASCs after osteogenic induction.Conclusion: Our study demonstrated that experiment periodontitis decreased the expression of TNF-a and IL-1β in adipose tissue in rats. Experiment periodontitis promoted the proliferation and wound-3 healing ability of ASCs, but obviously inhibited the osteogenic differentiation of ASCs.

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Section: Discussionmentioning

confidence: 71%

Effects of experimental periodontitis on proliferation and osteogenic differentiation of adipose-derived stem cells in rats

Huang

Cheng

Fan

et al. 2019

Preprint

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“…The regeneration of periodontal tissue requires the restoration of cementum, periodontal ligament, and alveolar bone. 65 A number of surgical regenerative techniques including osseous grafts, [3][4][5] alloplast grafts, 3 guided tissue regeneration (GTR) technique, 1,3,5,6 enamel matrix proteins, [8][9][10] chemical mediators, 11 have been developed to regenerate the lost periodontal tissues. Although these treatment modalities have been widely accepted, the amount of tissue regenerated could not be well-predicted in cases of advanced periodontal defects.…”

Section: Discussionmentioning

confidence: 99%

“…These results indicate that ADMPC have immune-modulation capability that can induce periodontal tissue regeneration by allogeneic transplantation. 65…”

Section: Discussionmentioning

confidence: 99%

Exploring the potential of human adipocytes in periodontal regeneration: A review

Ranjit¹,

Manandhar²,

Bista³

2020

J. Gandaki Med. Coll. Nepal

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Stem cells, initially identified in embryonic tissues and later in numerous adult tissues, tend to possess the potentiality to differentiate into various cell types. Though most flexible of all stem cell lines, ethical issues restrict the use of embryonic cells. Furthermore, induced pluripotent stem cells (iPS) and adult stem cells (e.g: bone marrow stroma) can also be used. However, procurement of autologous bone marrow has its potential limitations. An alternate source of autologous adult stem cells which can be procured in large quantities, under local anesthesia, with minimal discomfort would be of keen interest. In the present context, human adult adipose tissue may be the best appropriate alternative source of mesenchymal stem cells. Studies have shown that adipose stem cells (ASCs) extracted from subcutaneous human adult adipose tissue tend to contain heterogeneous cell population called stromal vascular fraction (SVF). It may be used directly or cultured in for selection and expansion of an adherent population, and hence, they are called ASCs. The adipose tissue, obtained by suction-assisted lipectomy (i.e., liposuction), are processed to obtain a fibroblast-like population of cells, also called processed lipoaspirate (PLA). PLA cells has the potentiality to differentiate in vitro into adipogenic, chondrogenic, myogenic, and osteogenic cells in the presence of lineage-specific induction factors. This attributable feature of ASCs may be of significant importance in future clinical cell-based therapy for periodontal disease as well. This review describes current knowledge & recent advances in ASCs & their application. This review describes current knowledge and recent advances in ASCs and their application in periodontal regeneration.

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“…In experimental dog models, autologous BMMSCs or xenogeneic periodontal ligament MSCs have proven beneficial in periodontal ligament reconstruction, when combined with the growth factors (205), fibrin glue, PRP (206), ephrinB2-a membrane protein regulating bone homeostasis (204) or with a construct of porous biphasic calcium phosphate (203). Allogeneic ADMSCs alone are also capable of inducing periodontal tissue regeneration in the mini pig periodontal defect model (207). For dental pulp regeneration, autologous (200) and allogeneic (201) stem cells from dental pulp or autologous BMMSCs (208) were efficient in dental pulp regeneration in canine models.…”

Section: Orodental Diseasesmentioning

confidence: 99%

Stem Cells in Veterinary Medicine—Current State and Treatment Options

et al. 2020

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Regenerative medicine is a branch of medicine that develops methods to grow, repair, or replace damaged or diseased cells, organs or tissues. It has gained significant momentum in recent years. Stem cells are undifferentiated cells with the capability to self-renew and differentiate into tissue cells with specialized functions. Stem cell therapies are therefore used to overcome the body's inability to regenerate damaged tissues and metabolic processes after acute or chronic insult. The concept of stem cell therapy was first introduced in 1991 by Caplan, who proposed that massive differentiation of cells into the desired tissue could be achieved by isolation, cultivation, and expansion of stem cells in in vitro conditions. Among different stem cell types, mesenchymal stem cells (MSC) currently seem to be the most suitable for therapeutic purposes, based on their simple isolation and culturing techniques, and lack of ethical issues regarding their usage. Because of their remarkable immunomodulatory abilities, MSCs are increasingly gaining recognition in veterinary medicine. Developments are primarily driven by the limitations of current treatment options for various medical problems in different animal species. MSCs represent a possible therapeutic option for many animal diseases, such as orthopedic, orodental and digestive tract diseases, liver, renal, cardiac, respiratory, neuromuscular, dermal, olfactory, and reproductive system diseases. Although we are progressively gaining an understanding of MSC behavior and their mechanisms of action, some of the issues considering their use for therapy are yet to be resolved. The aim of this review is first to summarize the current knowledge and stress out major issues in stem cell based therapies in veterinary medicine and, secondly, to present results of clinical usage of stem cells in veterinary patients.

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Periodontal Regeneration by Allogeneic Transplantation of Adipose Tissue Derived Multi-Lineage Progenitor Stem Cells in vivo

Cited by 46 publications

References 47 publications

Effects of experimental periodontitis on proliferation and osteogenic differentiation of adipose-derived stem cells in rats

Effects of experimental periodontitis on proliferation and osteogenic differentiation of adipose-derived stem cells in rats

Exploring the potential of human adipocytes in periodontal regeneration: A review

Stem Cells in Veterinary Medicine—Current State and Treatment Options

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