Periodontitis and Inflammatory Markers in Transplant Recipients

Shaqman, Murad; Ioannidou, Effie; Burleson, Joseph A.; Hull, David; Dongari-Bagtzoglou, Anna

doi:10.1902/jop.2010.090570

Cited by 15 publications

(23 citation statements)

References 36 publications

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“…The population investigated was a subset from a larger study that recruited 90 renal and cardiac transplant recipients and 72 controls (9,10). All study procedures were approved by the Institutional Review Boards from the University of Connecticut Health Center and Hartford Hospital.…”

Section: Methodsmentioning

confidence: 99%

“…Medical records of transplant subjects were reviewed, and all relevant information was collected using a standardized data extraction form, as previously described (10). A self-reported medical history was obtained from control subjects.…”

Section: Methodsmentioning

confidence: 99%

“…Serum values of C-reactive protein (CRP) and interleukin-6 (IL-6) were determined in both groups via enzyme-linked immunosorbent assays. Descriptive statistics on the entire study population were previously reported (10).…”

Section: Methodsmentioning

confidence: 99%

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Transplantation-Associated Long-Term Immunosuppression Promotes Oral Colonization by Potentially Opportunistic Pathogens without Impacting Other Members of the Salivary Bacteriome

Diaz

Hong

Frias-Lopez³

et al. 2013

Clin Vaccine Immunol

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e Solid-organ transplant recipients rely on pharmacological immunosuppression to prevent allograft rejection. The effect of such chronic immunosuppression on the microflora at mucosal surfaces is not known. We evaluated the salivary bacterial microbiome of 20 transplant recipients and 19 nonimmunosuppressed controls via 454 pyrosequencing of 16S rRNA gene amplicons. Alpha-diversity and global community structure did not differ between transplant and control subjects. However, principal coordinate analysis showed differences in community membership. Taxa more prevalent in transplant subjects included operational taxonomic units (OTUs) of potentially opportunistic Gammaproteobacteria such as Klebsiella pneumoniae, Pseudomonas fluorescens, Acinetobacter species, Vibrio species, Enterobacteriaceae species, and the genera Acinetobacter and Klebsiella. Transplant subjects also had increased proportions of Pseudomonas aeruginosa, Acinetobacter species, Enterobacteriaceae species, and Enterococcus faecalis, among other OTUs, while genera with increased proportions included Klebsiella, Acinetobacter, Staphylococcus, and Enterococcus. Furthermore, in transplant subjects, the dose of the immunosuppressant prednisone positively correlated with bacterial richness, while prednisone and mycophenolate mofetil doses positively correlated with the prevalence and proportions of transplant-associated taxa. Correlation network analysis of OTU relative abundance revealed a cluster containing potentially opportunistic pathogens as transplant associated. This cluster positively correlated with serum levels of C-reactive protein, suggesting a link between the resident flora at mucosal compartments and systemic inflammation. Network connectivity analysis revealed opportunistic pathogens as highly connected to each other and to common oral commensals, pointing to bacterial interactions that may influence colonization. This work demonstrates that immunosuppression aimed at limiting Tcell-mediated responses creates a more permissive oral environment for potentially opportunistic pathogens without affecting other members of the salivary bacteriome.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Transplantation-Associated Long-Term Immunosuppression Promotes Oral Colonization by Potentially Opportunistic Pathogens without Impacting Other Members of the Salivary Bacteriome

Diaz

Hong

Frias-Lopez³

et al. 2013

Clin Vaccine Immunol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Renal transplant recipients show also higher levels of markers of inflammation, such as interleukin 6, tumor necrosis factor alpha, and C‐reactive protein (Blach et al , 2009; Shaqman et al , 2010), which are generally raised in patients with periodontitis (Somma et al , 2010). However, high levels of markers of inflammation are not correlated with periodontal disease development and severity in transplant patients (Shaqman et al , 2010). Thus, it seems unlikely that the transient increase in periodontal pathogens and the high serum level of inflammatory markers are associated with an increased risk of periodontal disease in SOT recipients.…”

Section: Oral Infectionsmentioning

confidence: 99%

“…Renal transplant recipients show also higher levels of markers of inflammation, such as interleukin 6, tumor necrosis factor alpha, and C-reactive protein (Blach et al, 2009;Shaqman et al, 2010), which are generally raised in patients with periodontitis (Somma et al, 2010). However, high levels of markers of inflammation are not correlated with periodontal disease development and severity in transplant patients .…”

Section: Periodontal Diseasementioning

confidence: 99%