Perioperative systemic IL‐6 and immune‐adipose‐ metabolism transcription in tumour and tumour adjacent breast cancer

Abstract

Surgical resection is the primary treatment approach for patients with breast cancer. Despite optimal multimodal treatment, metastatic recurrence remains a risk. Surgery‐mediated systemic inflammation and local tissue inflammation generate an immunosuppressive and wound‐healing environment that may accelerate cancer recurrence and metastasis post‐operatively. Investigating the impact of surgery on local and systemic inflammation may provide knowledge for improvement of patient prognosis and treatment opportunities. Systemic cytokines were quantified in the blood plasma of patients with breast cancer pre‐operatively, early post‐operatively, and late post‐operatively. Early post‐operative levels of IL‐6 were significantly elevated in patients who underwent mastectomy compared with wide local excision. Post‐operative IL‐6 levels correlate with clinicopathological features (age and BMI). The transcriptomes of local matched tumour and normal tumour adjacent (normal) breast tissue, from patients with breast cancer, were analysed by RNA‐Seq. Elevated gene expressions of IL6, ADIPOQ, FABP4, LPL, PPARG, and CD36 in normal tissue were associated with worse overall survival of patients with ER‐positive breast cancer. In tissue with higher expression of IL6 and ADIPOQ, a higher abundance of M2‐like macrophage gene expression was identified. This study revealed perioperative systemic dynamics of inflammatory mediators and identified local immune‐adipose‐metabolism gene expression in tumour‐adjacent tissue associated with pro‐tumour function.