Periosteal Skeletal Stem and Progenitor Cells in Bone Regeneration

Perrin, Simon; Colnot, Céline

doi:10.1007/s11914-022-00737-8

Cited by 28 publications

(23 citation statements)

References 114 publications

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“…As we all know, periosteum is composed of brous connective tissue, which can be divided into inner and outer layers, and is the main source of bone stem/progenitor cells (SSPCS) for bone repair [24], with dense outer layer and loose inner layer. The outer layer is rich in broblasts, skeletal progenitor cells and some parts of osteoblasts, while the inner layer mainly contains more nerves, blood vessels and osteoblasts [25].…”

Section: Discussionmentioning

confidence: 99%

Isolation, culture and biological characteristics of duck periosteum stem cells in vitro

wang

liu

Zong

et al. 2023

Preprint

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Mesenchymal stem cells (MSCs), known as seed cells, have been shown to be useful in repairing damaged or diseased tissues and organs in the past. However, its clinical application in vivo is limited by individual differences and diversity of sources. In contrast, periosteum or periosteum-derived stem cells (PSCS) are more appropriate for fracture or cartilage damage. PC is also known as MSCs, but it is not clear whether PC is a class of cells of MSCs. Our objective was to isolate the PC of the periosteum of the duck embryo tibia and study its properties. The femoral periosteum of duck embryos at 12 days of age was easily collected, and the isolated cells showed typical stem cell morphology by enzyme digestion and adhesion method. Both immunofluorescence detection and RT-PCR showed that PSC could express CD90, CD29, CD166 and CD44 on the surface of MSCs, but not CD34 on the surface of hematopoietic stem cells. Under different culture conditions, PSC was proved to have the ability to differentiate into adipocytes, osteoblasts and chondroblasts in vitro. In addition, PC also has a strong ability to proliferate and self-renewal ability. In conclusion, our study suggests that PSCS may contain MSCs, which can be used as one of the candidate resources for clinical treatment of bone diseases.

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Section: Discussionmentioning

confidence: 99%

Isolation, culture and biological characteristics of duck periosteum stem cells in vitro

wang

liu

Zong

et al. 2023

Preprint

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“…In fact, current methods to extract cells from the periosteum are based on mechanical scraping and subsequent enzymatic digestion; alternatively, periosteal cells have been isolated by means of direct ex-vivo tissue culturing of the explanted periosteum ( Roberts et al, 2015 ). To track periosteal SSPCs, studies have used countless markers (for a complete list, we suggest looking at ( Perrin and Colnot, 2022 )). Gli1 and Prrx1, which, as mentioned above, have been used to identify SSPCs of the bone marrow, along with Axis inhibition protein 2 (Axin2) and Cathepsin K (Ctsk) have also been utilized to mark SSPCs of the periosteum.…”

Section: Identification Of Sspcs: a Fundamental Issuementioning

confidence: 99%

Age-associated declining of the regeneration potential of skeletal stem/progenitor cells

Mancinelli

Intini

2023

Front. Physiol.

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Bone fractures represent a significant health burden worldwide, mainly because of the rising number of elderly people. As people become older, the risk and the frequency of bone fractures increase drastically. Such increase arises from loss of skeletal integrity and is also associated to a reduction of the bone regeneration potential. Central to loss of skeletal integrity and reduction of regeneration potential are the skeletal stem/progenitor cells (SSPCs), as they are responsible for the growth, regeneration, and repair of the bone tissue. However, the exact identity of the SSPCs has not yet been determined. Consequently, their functions, and especially dysfunctions, during aging have never been fully characterized. In this review, with the final goal of describing SSPCs dysfunctions associated to aging, we first discuss some of the most recent findings about their identification. Then, we focus on how SSPCs participate in the normal bone regeneration process and how aging can modify their regeneration potential, ultimately leading to age-associated bone fractures and lack of repair. Novel perspectives based on our experience are also provided.

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“…Bone regeneration is a well-regulated process requiring the recruitment of skeletal stem/progenitor cells (SSPCs) from adjacent tissues. Among them periosteum is a connective tissue that surrounds the external surface of bones and has been widely described as physiologically essential for bone development and healing ( Duchamp de Lageneste and Colnot, 2019 ; Julien et al, 2022 ; Perrin and Colnot, 2022 ). This membrane consists of a fibrous outer layer composed of type I collagen and a highly cellular inner layer composed of fibroblasts, osteoprogenitor cells and mesenchymal stem cells ( Squier, Ghoneim, and Kremenak, 1990 ; Dwek, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Collagen molecular organization preservation in human fascia lata and periosteum after tissue engineering

Vettese,

Manon,

Chretien

et al. 2024

Front. Bioeng. Biotechnol.

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Large bone defect regeneration remains a major challenge for orthopedic surgeons. Tissue engineering approaches are therefore emerging in order to overcome this limitation. However, these processes can alter some of essential native tissue properties such as intermolecular crosslinks of collagen triple helices, which are known for their essential role in tissue structure and function. We assessed the persistence of extracellular matrix (ECM) properties in human fascia lata (HFL) and periosteum (HP) after tissue engineering processes such as decellularization and sterilization. Harvested from cadaveric donors (N = 3), samples from each HFL and HP were decellularized following five different chemical protocols with and without detergents (D1-D4 and D5, respectively). D1 to D4 consisted of different combinations of Triton, Sodium dodecyl sulfate and Deoxyribonuclease, while D5 is routinely used in the institutional tissue bank. Decellularized HFL tissues were further gamma-irradiated (minimum 25 kGy) in order to study the impact of sterilization on the ECM. Polarized light microscopy (PLM) was used to estimate the thickness and density of collagen fibers. Tissue hydration and content of hydroxyproline, enzymatic crosslinks, and non-enzymatic crosslinks (pentosidine) were semi-quantified with Raman spectroscopy. ELISA was also used to analyze the maintenance of the decorin (DCN), an important small leucine rich proteoglycan for fibrillogenesis. Among the decellularization protocols, detergent-free treatments tended to further disorganize HFL samples, as more thin fibers (+53.7%) and less thick ones (−32.6%) were recorded, as well as less collagen enzymatic crosslinks (−25.2%, p = 0.19) and a significant decrease of DCN (p = 0.036). GAG content was significantly reduced in both tissue types after all decellularization protocols. On the other hand, HP samples were more sensitive to the D1 detergent-based treatments, with more disrupted collagen organization and greater, though not significant loss of enzymatic crosslinks (−37.4%, p = 0.137). Irradiation of D5 HFL samples, led to a further and significant loss in the content of enzymatic crosslinks (−29.4%, p = 0.037) than what was observed with the decellularization process. Overall, the results suggest that the decellularization processes did not significantly alter the matrix. However, the addition of a gamma-irradiation is deleterious to the collagen structural integrity of the tissue.

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Periosteal Skeletal Stem and Progenitor Cells in Bone Regeneration

Cited by 28 publications

References 114 publications

Isolation, culture and biological characteristics of duck periosteum stem cells in vitro

Isolation, culture and biological characteristics of duck periosteum stem cells in vitro

Age-associated declining of the regeneration potential of skeletal stem/progenitor cells

Collagen molecular organization preservation in human fascia lata and periosteum after tissue engineering

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