Periosteum-derived cells respond to mechanical stretch and activate Wnt andBMP signaling pathways

Ito, Ryohei; Matsumiya, Tomoh; Kon, Tomoya; Narita, Norihiko; Kubota, Kosei; Sakaki, Hirotaka; Ozaki, Taku; Imaizumi, Tadaatsu; Kobayashi, Wataru; Hiroto, Kimura

doi:10.2220/biomedres.35.69

Cited by 18 publications

(10 citation statements)

References 37 publications

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“…At 2-week, the signaling pathways that were related to the up-regulation in osteogenesis-associated gene expression are Tgfβ/Bmp, Wnt and Notch. These signaling pathways have all been involved in bone formation and development 29 – 34 , and are comprehensively understood due to their important roles in the process of osteoblast differentiation 35 – 39 . As for bone regeneration, Bmp signaling appears to play a key role, with BMP2 being up-regulated in week-2.…”

Section: Discussionmentioning

confidence: 99%

Microarray gene expression of periosteum in spontaneous bone regeneration of mandibular segmental defects

Pan

et al. 2017

Sci Rep

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Spontaneous bone regeneration could occur to reestablish mandibular bony continuity in patients who underwent partial or total mandibulectomy for tumors with periosteum-preserving. However, scarce data is available related to the precise role of periosteum in this bone regeneration. Therefore we aimed to investigate the gene expression of periosteum that were involved in the mandibular bone regeneration. Mandibular segmental defects were created in six mini-pigs with periosteum preserved. The periosteum of defects and control site were harvested at 1 and 2 weeks. Gene ontology (GO) analysis showed that the mechanisms concerning immature wound healing were clearly up-regulated at week 1. In contrast, by week-2, the GO categories of skeletal development, ossification and bone mineralization were significantly over-represented at week-2 with several genes encoding cell differentiation, extracellular matrix formation, and anatomical structure development. Furthermore, Tgfβ/Bmp, Wnt and Notch signaling were all related to the osteogenic process in this study. Besides osteogenesis, genes related to angiogenesis and neurogenesis were also prominent at week-2. These findings revealed that the gene expression profile of the periosteum’s cells participating in bone regeneration varied in different time points, and numbers of candidate genes that differentially expressed during early healing stages of intramembranous bone regeneration were suggested.

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Section: Discussionmentioning

confidence: 99%

Microarray gene expression of periosteum in spontaneous bone regeneration of mandibular segmental defects

Pan

et al. 2017

Sci Rep

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“…Among the biomaterials described for tendon tissue engineering [14], a promising emerging strategy is the use of a complex biomimetic matrix with a hydrogel component and extracellular matrix mimicking properties [15,16]. Tenocyte precursors can be harvested from different sources, including periosteum [17,18], bone marrow [19][20][21], tendon [21,22], and adipose tissue [21,23]. To overcome the intrinsic poor mechanical properties of the hydrogel, they can be merged with more force resistant biopolymers.…”

Section: Introductionmentioning

confidence: 99%

Tendon and Cytokine Marker Expression by Human Bone Marrow Mesenchymal Stem Cells in a Hyaluronate/Poly-Lactic-Co-Glycolic Acid (PLGA)/Fibrin Three-Dimensional (3D) Scaffold

Ciardulli

Marino

Lovecchio

et al. 2020

Cells

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We developed a (three-dimensional) 3D scaffold, we named HY-FIB, incorporating a force-transmission band of braided hyaluronate embedded in a cell localizing fibrin hydrogel and poly-lactic-co-glycolic acid (PLGA) nanocarriers as transient components for growth factor controlled delivery. The tenogenic supporting capacity of HY-FIB on human-Bone Marrow Mesenchymal Stem Cells (hBM-MSCs) was explored under static conditions and under bioreactor-induced cyclic strain conditions. HY-FIB elasticity enabled to deliver a mean shear stress of 0.09 Pa for 4 h/day. Tendon and cytokine marker expression by hBM-MSCs were studied. Results: hBM-MSCs embedded in HY-FIB and subjected to mechanical stimulation, resulted in a typical tenogenic phenotype, as indicated by type 1 Collagen fiber immunofluorescence. RT-qPCR showed an increase of type 1 Collagen, scleraxis, and decorin gene expression (3-fold, 1600-fold, and 3-fold, respectively, at day 11) in dynamic conditions. Cells also showed pro-inflammatory (IL-6, TNF, IL-12A, IL-1β) and anti-inflammatory (IL-10, TGF-β1) cytokine gene expressions, with a significant increase of anti-inflammatory cytokines in dynamic conditions (IL-10 and TGF-β1 300-fold and 4-fold, respectively, at day 11). Mechanical signaling, conveyed by HY-FIB to hBM-MSCs, promoted tenogenic gene markers expression and a pro-repair cytokine balance. The results provide strong evidence in support of the HY-FIB system and its interaction with cells and its potential for use as a predictive in vitro model.

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“…So far, one of the most promising strategies is represented by the use of biomimetic matrix scaffolds (preferably hydrogels) capable of mimicking the extracellular matrix and providing a support for the growth, differentiation and organization of tenocyte precursors . Such precursors, as demonstrated in the recent past, may be harvested from different sources including periosteum , bone marrow , tendon itself and adipose tissue . Different biomaterial‐based systems have been developed and tested for the fabrication of artificial tendon tissue, including natural matrices , hybrid matrices (featuring natural and synthetic components) and fully synthetic matrices .…”

Section: Introductionmentioning

confidence: 99%

Combination of biochemical and mechanical cues for tendon tissue engineering

Testa

Costantini

Fornetti

et al. 2017

J Cellular Molecular Medi

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Tendinopathies negatively affect the life quality of millions of people in occupational and athletic settings, as well as the general population. Tendon healing is a slow process, often with insufficient results to restore complete endurance and functionality of the tissue. Tissue engineering, using tendon progenitors, artificial matrices and bioreactors for mechanical stimulation, could be an important approach for treating rips, fraying and tissue rupture. In our work, C3H10T1/2 murine fibroblast cell line was exposed to a combination of stimuli: a biochemical stimulus provided by Transforming Growth Factor Beta (TGF‐β) and Ascorbic Acid (AA); a three‐dimensional environment represented by PEGylated‐Fibrinogen (PEG‐Fibrinogen) biomimetic matrix; and a mechanical induction exploiting a custom bioreactor applying uniaxial stretching. In vitro analyses by immunofluorescence and mechanical testing revealed that the proposed combined approach favours the organization of a three‐dimensional tissue‐like structure promoting a remarkable arrangement of the cells and the neo‐extracellular matrix, reflecting into enhanced mechanical strength. The proposed method represents a novel approach for tendon tissue engineering, demonstrating how the combined effect of biochemical and mechanical stimuli ameliorates biological and mechanical properties of the artificial tissue compared to those obtained with single inducement.

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Periosteum-derived cells respond to mechanical stretch and activate Wnt andBMP signaling pathways

Cited by 18 publications

References 37 publications

Microarray gene expression of periosteum in spontaneous bone regeneration of mandibular segmental defects

Microarray gene expression of periosteum in spontaneous bone regeneration of mandibular segmental defects

Tendon and Cytokine Marker Expression by Human Bone Marrow Mesenchymal Stem Cells in a Hyaluronate/Poly-Lactic-Co-Glycolic Acid (PLGA)/Fibrin Three-Dimensional (3D) Scaffold

Combination of biochemical and mechanical cues for tendon tissue engineering

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