Periostin and matrix stiffness combine to regulate myofibroblast differentiation and fibronectin synthesis during palatal healing

Nikoloudaki, Georgia; Snider, Paige; Simmons, Olga; Conway, Simon J.; Hamilton, David A.

doi:10.1016/j.matbio.2020.07.002

Cited by 29 publications

(25 citation statements)

References 102 publications

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“…Several lines of evidence have indicated that α-SMA-associated contraction is involved in ECM organization 67 , 70 , 71 . The up-regulation of α-SMA is also important for myofibroblast focal adhesion, maturation and adhesive capability 72 , 73 . In tubulointerstitial renal fibrosis, TGF-β1 enhances α-SMA expression in fibroblasts to form stress fibers and focal adhesion, resulting in the fibrotic tissue contraction 74 , 75 .…”

Section: Discussionmentioning

confidence: 99%

Effects of secretome derived from macrophages exposed to calcium oxalate crystals on renal fibroblast activation

et al. 2021

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The association between kidney stone disease and renal fibrosis has been widely explored in recent years but its underlying mechanisms remain far from complete understanding. Using label-free quantitative proteomics (nanoLC-ESI-LTQ-Orbitrap MS/MS), this study identified 23 significantly altered secreted proteins from calcium oxalate monohydrate (COM)-exposed macrophages (COM-MP) compared with control macrophages (Ctrl-MP) secretome. Functional annotation and protein-protein interactions network analysis revealed that these altered secreted proteins were involved mainly in inflammatory response and fibroblast activation. BHK-21 renal fibroblasts treated with COM-MP secretome had more spindle-shaped morphology with greater spindle index. Immunofluorescence study and gelatin zymography revealed increased levels of fibroblast activation markers (α-smooth muscle actin and F-actin) and fibrotic factors (fibronectin and matrix metalloproteinase-9 and -2) in the COM-MP secretome-treated fibroblasts. Our findings indicate that proteins secreted from macrophages exposed to COM crystals induce renal fibroblast activation and may play important roles in renal fibrogenesis in kidney stone disease.

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Section: Discussionmentioning

confidence: 99%

Effects of secretome derived from macrophages exposed to calcium oxalate crystals on renal fibroblast activation

et al. 2021

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“…At the same time, the GSEA analysis also had enriched entries of vascular smooth muscle contraction that interest us, indicating that it had an impact on contractility, including the myofibroblast marker protein α-SMA (also known as ACTA2) [ 26 ] ( Figure 4A ), it was also considered to be one of the most stable cytoskeletal components of myofibroblasts [ 27 ], it was found that the soft substrates did significantly down-regulate its expression ( Figure 4B ). Periostin regulates myofibroblast differentiation and is persistently overexpressed in abnormal scars and other benign fibrous tissues proliferating with fibroblasts [ 28 ], while soft substrates Inhibit its expression, also in the presence of TGF-β1 stimulation ( Figure 4C ), it showed that the soft substrates may inhibit the differentiation and contraction of hFFs, and combined with the soft substrates inhibiting the proliferation of hFFs, these phenomena have certain significance for the treatment of skin scarring, sclerosis, fibrosis and other diseases related to the proliferation of FBs.…”

Section: Resultsmentioning

confidence: 99%

Integrated PPI- and WGCNA-retrieval of hub gene signatures for soft substrates inhibition of human fibroblasts proliferation and differentiation

Zhou

Wang

et al. 2022

Aging

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Fibroblasts (FBs) are the most important functional cells in the process of wound repair, and their functions can be activated by different signals at the pathological site. Although wound repair is associated with microenvironmental stiffness, the effect of matrix stiffness on FBs remains elusive. In this study, TGF-β1 was used to mimic the fibrotic environment under pathological conditions. We found that the soft substrates made FBs slender compared with tissue culture plastic, and the main altered biological function was the inhibition of proliferation and differentiation ability. Through PPI and WGCNA analysis, 63 hub genes were found, including GADD45A, CDKN3, HIST2H3PS2, ACTB, etc., which may be the main targets of soft substrates affecting the proliferation and differentiation of FBs. Our findings not only provide a more detailed report on the effect of matrix stiffness on the function of human skin FBs, but also may provide new intervention ideas for improving scars and other diseases caused by excessive cell proliferation, with potential clinical application prospects.

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“…In vitro, palatal fibroblasts isolated from Pstn −/− mice exhibited reduced contractability, which was rescued by the exogenous addition of periostin, and reduced fibronectin synthesis. These effects were also modulated by the stiffness of the microenvironment via the integrin-β1/RhoA pathway, suggesting that periostin and the ECM stiffness act as important modulators of cell behaviour during palatal healing [ 66 ]. The contrasting effects of periostin among dermal, gingival and palatal tissues demonstrate the tissue-specificity of its bioactivity in relatively homologous tissues [ 59 ].…”

Section: Gla-containing Ecm Proteinsmentioning

confidence: 99%

“…Recently we showed that bigh3 mRNA signal is present in the normal, uninjured palate and is upregulated during wound healing. βigh3 message is present in the granulation tissue by day 3 post wounding, with mRNA levels peaking at day 6 after which they are gradually reduced [ 66 ]. βigh3 is also expressed in human PDL tissues.…”

Section: Gla-containing Ecm Proteinsmentioning

confidence: 99%

Functions of Matricellular Proteins in Dental Tissues and Their Emerging Roles in Orofacial Tissue Development, Maintenance, and Disease

Nikoloudaki

2021

IJMS

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Matricellular proteins (MCPs) are defined as extracellular matrix (ECM) associated proteins that are important regulators and integrators of microenvironmental signals, contributing to the dynamic nature of ECM signalling. There is a growing understanding of the role of matricellular proteins in cellular processes governing tissue development as well as in disease pathogenesis. In this review, the expression and functions of different MP family members (periostin, CCNs, TSPs, SIBLINGs and others) are presented, specifically in relation to craniofacial development and the maintenance of orofacial tissues, including bone, gingiva, oral mucosa, palate and the dental pulp. As will be discussed, each MP family member has been shown to have non-redundant roles in development, tissue homeostasis, wound healing, pathology and tumorigenesis of orofacial and dental tissues.

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Periostin and matrix stiffness combine to regulate myofibroblast differentiation and fibronectin synthesis during palatal healing

Cited by 29 publications

References 102 publications

Effects of secretome derived from macrophages exposed to calcium oxalate crystals on renal fibroblast activation

Effects of secretome derived from macrophages exposed to calcium oxalate crystals on renal fibroblast activation

Integrated PPI- and WGCNA-retrieval of hub gene signatures for soft substrates inhibition of human fibroblasts proliferation and differentiation

Functions of Matricellular Proteins in Dental Tissues and Their Emerging Roles in Orofacial Tissue Development, Maintenance, and Disease

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