Periostin induces epithelial‑to‑mesenchymal transition via the integrin α5β1/TWIST‑2 axis in cholangiocarcinoma

Sonongbua, Jumaporn; Siritungyong, Suchada; Thongchot, Suyanee; Kamolhan, Thanpawee; Utispan, Kusumawadee; Thuwajit, Peti; Pongpaibul, Ananya; Wongkham, Sopit; Thuwajit, Chanitra

doi:10.3892/or.2020.7485

Cited by 10 publications

(8 citation statements)

References 29 publications

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“…Twist2 has been demonstrated to be an important regulator and a poor diagnosis marker in some cancers [41][42][43][44], such as gastric cancer [42], cholangiocarcinoma [41], glioma [43], etc. Importantly, it has been revealed that it could accelerate cell proliferation, metastasis, and epithelialmesenchymal transition (EMT) in HCC [30,31,45].…”

Section: Discussionmentioning

confidence: 99%

The Elevated Circ_0067835 Could Accelerate Cell Proliferation and Metastasis via miR-1236-3p/Twist2 Axis in Hepatocellular Carcinoma

Chen

2022

BioMed Research International

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Hepatocellular carcinoma (HCC) is a malignant cancer with leading mortality worldwide. Circ_0067835 is a circRNA which plays an important role in various kinds of tumor, while the potential functions of circ_0067835 in HCC remains unclear. In this study, our results of microarray and real-time PCR (RT-PCR) showed that it was obviously elevated in human HCC tumor tissues and HCC cell lines. Inhibition of circ_0067835 restrained cell proliferation and migration in vitro. Furthermore, miR-1236-3p was decreased in tumor samples, and it was indicated to be a target of circ_0067835. Moreover, Twist2 was established to be elevated in HCC tissues, and we identified it as the direct target of miR-1236-3p. Finally, we found that knockdown of miR-1236-3p could reverse the circ_0067835 inhibition effects in HCC cells. In conclusion, our study demonstrated that circ_0067835 contributed to promoting hepatocellular carcinoma cell proliferation and metastasis through downregulating miR-1236-3p expression and then elevating Twist2 expression, which might provide a new vision for HCC patients.

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Section: Discussionmentioning

confidence: 99%

The Elevated Circ_0067835 Could Accelerate Cell Proliferation and Metastasis via miR-1236-3p/Twist2 Axis in Hepatocellular Carcinoma

Chen

2022

BioMed Research International

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“…POSTN encodes for periostin, a multifunctional ECM protein that contributes to the remodeling of the tumor microenvironment during tumor progression [36]. In CCA and hepatoblastoma, overexpressed periostin was found to induce cell migration and epithelial-to-mesenchymal transition (EMT) features [37,38]. On the other hand, PUC-GBC1 cells exhibited a slower growth rate and an enhanced in vivo tumorigenic capability.…”

Section: Discussionmentioning

confidence: 99%

Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor

et al. 2020

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Background: Gallbladder cancer (GBC) is the most common tumor of the biliary tract. The incidence of GBC shows a large geographic variability, being particularly frequent in Native American populations. In Chile, GBC represents the second cause of cancer-related death among women. We describe here the establishment of three novel cell lines derived from the ascitic fluid of a Chilean GBC patient, who presented 46% European, 36% Mapuche, 12% Aymara and 6% African ancestry.Results: After immunocytochemical staining of the primary cell culture, we isolated and comprehensively characterized three independent clones (PUC-GBC1, PUC-GBC2 and PUC-GBC3) by short tandem repeat DNA profiling and RNA sequencing as well as karyotype, doubling time, chemosensitivity, in vitro migration capability and in vivo tumorigenicity assay. Primary culture cells showed high expression of CK7, CK19, CA 19-9, MUC1 and MUC16, and negative expression of mesothelial markers. The three isolated clones displayed an epithelial phenotype and an abnormal structure and number of chromosomes. RNA sequencing confirmed the increased expression of cytokeratin and mucin genes, and also of TP53 and ERBB2 with some differences among the three cells lines, and revealed a novel exonic mutation in NF1. The PUC-GBC3 clone was the most aggressive according to histopathological features and the tumorigenic capacity in NSG mice. Conclusions:The first cell lines established from a Chilean GBC patient represent a new model for studying GBC in patients of Native American descent.

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“…Periostin (POSTN), a matricellular N-glycoprotein, is implicated in the EMT process, and is crucial for the collagen fibrillogenesis process [ 184 , 185 ] and wound healing [ 186 , 187 ]. Cellular survival, motility, and adhesion are facilitated by POSTN-activated signalling pathways, which are essential for tumour growth, angiogenesis, invasion, and metastasis [ 188 , 189 , 190 ].…”

Section: Cafs Biomarkers: a Mixed Bagmentioning

confidence: 99%

Dual Role of Fibroblasts Educated by Tumour in Cancer Behavior and Therapeutic Perspectives

Toledo

Picón-Ruiz

Marchal

et al. 2022

IJMS

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Tumours are complex systems with dynamic interactions between tumour cells, non-tumour cells, and extracellular components that comprise the tumour microenvironment (TME). The majority of TME’s cells are cancer-associated fibroblasts (CAFs), which are crucial in extracellular matrix (ECM) construction, tumour metabolism, immunology, adaptive chemoresistance, and tumour cell motility. CAF subtypes have been identified based on the expression of protein markers. CAFs may act as promoters or suppressors in tumour cells depending on a variety of factors, including cancer stage. Indeed, CAFs have been shown to promote tumour growth, survival and spread, and secretome changes, but they can also slow tumourigenesis at an early stage through mechanisms that are still poorly understood. Stromal–cancer interactions are governed by a variety of soluble factors that determine the outcome of the tumourigenic process. Cancer cells release factors that enhance the ability of fibroblasts to secrete multiple tumour-promoting chemokines, acting on malignant cells to promote proliferation, migration, and invasion. This crosstalk between CAFs and tumour cells has given new prominence to the stromal cells, from being considered as mere physical support to becoming key players in the tumour process. Here, we focus on the concept of cancer as a non-healing wound and the relevance of chronic inflammation to tumour initiation. In addition, we review CAFs heterogeneous origins and markers together with the potential therapeutic implications of CAFs “re-education” and/or targeting tumour progression inhibition.

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Periostin induces epithelial‑to‑mesenchymal transition via the integrin α5β1/TWIST‑2 axis in cholangiocarcinoma

Cited by 10 publications

References 29 publications

The Elevated Circ_0067835 Could Accelerate Cell Proliferation and Metastasis via miR-1236-3p/Twist2 Axis in Hepatocellular Carcinoma

The Elevated Circ_0067835 Could Accelerate Cell Proliferation and Metastasis via miR-1236-3p/Twist2 Axis in Hepatocellular Carcinoma

Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor

Dual Role of Fibroblasts Educated by Tumour in Cancer Behavior and Therapeutic Perspectives

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