Periostin mediates cigarette smoke extract-induced proliferation and migration in pulmonary arterial smooth muscle cells

Wang, Xiaodong; Li, Fang; Ma, Dongbo; Xiang, Dingcheng; Zhang, Hui; Gao, Jia Bao; Li, Hao; Liu, Dandan; Wang, Jing

doi:10.1016/j.biopha.2016.07.007

Cited by 13 publications

(8 citation statements)

References 26 publications

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“…Recent evidence points to an important role of components of the ECM from both a biomarker and a therapeutic prospective in PAH . The most upregulated gene in severe PAH was indeed periostin, another matricellular protein involved in response to vascular injury and regulation of SMC proliferation . Chronic normobaric hypoxic exposure for 2 weeks significantly increased the mRNA level of periostin in the rat lung and hypoxia‐induced periostin expression was localized to PA‐SMC of hypertrophic PA.…”

Section: Discussionmentioning

confidence: 99%

Osteopontin lung gene expression is a marker of disease severity in pulmonary arterial hypertension

et al. 2019

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Background and objective: Osteopontin (OPN) is a pleiotropic cytokine involved in the proliferation of pulmonary artery smooth muscle cells (PA-SMC). OPN is upregulated in the lungs of patients with pulmonary hypertension (PH) associated with pulmonary fibrosis, suggesting that the lung is a source of OPN. We hypothesized that OPN lung expression is elevated in Group I pulmonary arterial hypertension (PAH) and is correlated to haemodynamics. Methods: Microarray analysis (Affymetrix) was performed after RNA was extracted from explanted lungs in 15 patients with Group I PAH who underwent lung transplantation (LTx) and 11 normal controls. PA pressure levels were recorded intraoperatively, immediately before starting LTx. Serum OPN levels were measured in subjects with PAH, Group II PH and normal controls on the day of right heart catheterization. Results: OPN was among the top five upregulated genes in PAH compared to normal controls, which was confirmed by reverse transcription polymerase chain reaction (RT-PCR). OPN expression was similar and equally elevated in different subtypes of PAH. A strong significant correlation was observed between mean pulmonary arterial pressure and OPN gene expression. Ingenuity pathway analysis showed the involvement of OPN in functions and networks relevant to angiogenesis, cell death and proliferation of PA-SMC. OPN serum levels did not differ in subjects with Group I PAH and Group II PH. Conclusion: In the lungs of patients with severe PAH, OPN is highly expressed and the level of expression is significantly correlated to disease severity. OPN may play an important role in the vascular remodelling process of PAH. SUMMARY AT A GLANCEOsteopontin (OPN), a pleiotropic cytokine, was identified among the top five upregulated genes in the lung explants from patients with Group I pulmonary arterial hypertension, compared to normal controls. Its expression correlated strongly to haemodynamic severity. Ingenuity pathway analysis showed the involvement of OPN in functions and networks relevant to vascular remodelling.

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Section: Discussionmentioning

confidence: 99%

Osteopontin lung gene expression is a marker of disease severity in pulmonary arterial hypertension

et al. 2019

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“…It has been reported that Pn is secreted not only from fibrocytes or fibroblasts but also from PASMCs in the lung under stressed condition[9,30,34,35]. Given that Pn is a marker for proliferation in these cells[36,37,38], Pn-positive cells would be responded cells for the pathological stress and have a pivotal role on perivascular cellular proliferation and vascular remodeling in the lung with PAH. Pn-expressing cell-targeted inhibition of TGF-β signaling (Pn-Cre/ Tgfbr1 fl/fl mice) effectively attenuated the development of PAH under hypoxic condition, suggesting that Pn-positive cells are crucial for TGF-β-mediated vascular remodeling in PAH.…”

Section: Discussionmentioning

confidence: 99%

Periostin-expressing cell-specific transforming growth factor-β inhibition in pulmonary artery prevents pulmonary arterial hypertension

et al. 2019

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Transforming growth factor beta (TGF-β) has been shown to play a critical role in pathogenesis of pulmonary arterial hypertension (PAH) although the precise role of TGF-β signaling remains uncertain. A recent report has shown that periostin (Pn) is one of the most upregulated proteins in human PAH lung compared with healthy lungs. We established type I TGF-β receptor knockout mice specifically with Pn expressing cell (Pn-Cre/ Tgfb1 fl/fl mice). Increases in PA pressure and pulmonary artery muscularization were induced by hypoxia of 10% oxygen for 4 weeks. Lung Pn expression was markedly induced by 4 week-hypoxia. Pn-Cre/ Tgfb1 fl/fl mice showed lower right ventricular pressure elevation, inhibition of PA medial thickening. Fluorescent co-immunostaining showed that Smad3 activation in Pn expressing cell is attenuated. These results suggest that TGF-β signaling in Pn expressing cell may have an important role in the pathogenesis of PAH by controlling medial thickening.

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“…In myocardial infarction model rats, ERK1/2 phosphorylation mediates TGF-β1-induced cardiac fibrosis via Rho-kinase 1 activation but not the JNK or p38 MAPK pathway [44]. ERK1/2 also mediates cigarette smoke extract-induced periostin expression in pulmonary arterial smooth muscle cells [45]. In a dilated cardiomyopathy mouse model overexpressing the Fas ligand (an inducer of apoptosis via caspase 3 activation), Fas ligand-activated periostin expression is mediated by the ERK1/2 pathway [46,47].…”

Section: Roles Of Erk1/2 and P38 Mapk In Periostin Production In Cmentioning

confidence: 99%

Roles of Cyclic AMP Response Element Binding Activation in the ERK1/2 and p38 MAPK Signalling Pathway in Central Nervous System, Cardiovascular System, Osteoclast Differentiation and Mucin and Cytokine Production

Koga

Tsurumaki

Aoki‐Saito

et al. 2019

IJMS

120

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There are many downstream targets of mitogen-activated protein kinase (MAPK) signalling that are involved in neuronal development, cellular differentiation, cell migration, cancer, cardiovascular dysfunction and inflammation via their functions in promoting apoptosis and cell motility and regulating various cytokines. It has been reported that cyclic AMP response element-binding protein (CREB) is phosphorylated and activated by cyclic AMP signalling and calcium/calmodulin kinase. Recent evidence also points to CREB phosphorylation by the MAPK signalling pathway. However, the specific roles of CREB phosphorylation in MAPK signalling have not yet been reviewed in detail. Here, we describe the recent advances in the study of this MAPK-CREB signalling axis in human diseases. Overall, the crosstalk between extracellular signal-related kinase (ERK) 1/2 and p38 MAPK signalling has been shown to regulate various physiological functions, including central nervous system, cardiac fibrosis, alcoholic cardiac fibrosis, osteoclast differentiation, mucin production in the airway, vascular smooth muscle cell migration, steroidogenesis and asthmatic inflammation. In this review, we focus on ERK1/2 and/or p38 MAPK-dependent CREB activation associated with various diseases to provide insights for basic and clinical researchers.

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Periostin mediates cigarette smoke extract-induced proliferation and migration in pulmonary arterial smooth muscle cells

Cited by 13 publications

References 26 publications

Osteopontin lung gene expression is a marker of disease severity in pulmonary arterial hypertension

Osteopontin lung gene expression is a marker of disease severity in pulmonary arterial hypertension

Periostin-expressing cell-specific transforming growth factor-β inhibition in pulmonary artery prevents pulmonary arterial hypertension

Roles of Cyclic AMP Response Element Binding Activation in the ERK1/2 and p38 MAPK Signalling Pathway in Central Nervous System, Cardiovascular System, Osteoclast Differentiation and Mucin and Cytokine Production

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