Periostin modulates extracellular matrix behavior in tendons

Rolnick, Kevin; Choe, Joshua A.; Leiferman, Ellen M.; Kondratko-Mittnacht, Jaclyn; Clements, Anna E. B.; Baer, Geoffrey S.; Jiang, Peng; Vanderby, Ray; Chamberlain, Connie S.

doi:10.1016/j.mbplus.2022.100124

Cited by 7 publications

(2 citation statements)

References 28 publications

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“…The top three markers of cluster 5 were known tendon-associated collagens, COL1A1 , COL12A1 , and COL14A1 , 54 , 62 , 63 albeit being listed as markers for other tendon clusters ( Table S2 ). Other markers of cluster 5 known to be involved in tendon function included POSTN (coding for the secreted extracellular matrix protein, periostin) 64 and SPARC (coding for the secreted protein acidic and cysteine rich). 45 One striking observation was that the main recognized tendon markers, SCX and TNMD , were not expressed in the same tendon region; TNMD transcripts appeared to be enriched in the tendon core, while SCX transcripts were more homogenously distributed within tendons ( Figures 6 L–6N).…”

Section: Resultsmentioning

confidence: 99%

Limb connective tissue is organized in a continuum of promiscuous fibroblast identities during development

Hirsinger,

Blavet,

Bonnin

et al. 2024

iScience

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Section: Resultsmentioning

confidence: 99%

Limb connective tissue is organized in a continuum of promiscuous fibroblast identities during development

Hirsinger,

Blavet,

Bonnin

et al. 2024

iScience

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“…The top three markers of cluster 5 were known tendon-associated collagens, COL1A1, COL12A1 and COL14A1 (Lejard et al, 2011, Guerquin et al, 2013, Havis et al, 2014, albeit being listed as markers for other Tendon clusters, as well (Table S2). Other markers of cluster 5 known to be involved in tendon function included POSTN (coding for the secreted extracellular matrix protein, periostin) (Rolnick et al, 2022) and SPARC (coding for the secreted protein acidic and cysteine rich) (Wang et al, 2021). One striking observation was that the main recognized tendon markers, SCX and TNMD, were not expressed in the same tendon region; TNMD transcripts appeared to be enriched in the tendon core, while SCX transcripts were more homogenously distributed across the tendon (Figure 6K-N).…”

Section: Mct Clusters Map To Concentric Fibroblast Layersmentioning

confidence: 99%

Limb connective tissue is organized in a continuum of promiscuous fibroblast identities during development

Hirsinger,

Blavet,

Bonnin

et al. 2024

Preprint

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Tight developmental coordination of muscle attachments with skeletal muscle is fundamental for building functional limbs and allowing locomotion. Muscle attachments include connective tissue fibroblasts of tendon and muscle connective tissue. Although muscle attachments play critical roles in development, our understanding of connective tissue fibroblast developmental programs lags behind that of other components of the musculoskeletal system, mainly because fibroblasts are highly heterogeneous and poorly characterized. Combining single-cell RNA-sequencing-based strategies including trajectory inference andin situhybridization analyses, we address the diversity of connective tissue fibroblasts and their developmental trajectories during chicken limb fetal development. We show that fibroblasts switch from a program providing positional information to a program of lineage diversification at the onset of fetal period. Muscle connective tissue and tendon contain several fibroblast populations that emerge asynchronously. Once the final muscle pattern is set,in silicofibroblast populations that are close in transcriptional identity are found in neighbouring locations in limbs, prefiguring the adult fibroblast layers associated to muscle. We propose that the limb connective tissue is organised in a continuum of promiscuous fibroblast identities, allowing for the robust and efficient connection of the muscular system to bone and skin.HighlightsDissection of connective tissue heterogeneity using single-cell RNA-sequencing andin situRNA stainingIn silicofibroblast populations map to distinct fibroblast layers in limbsMolecular signatures of fetal fibroblast populations at the origin of limb adult connective tissueEpimysium and endomysium fibroblasts are transcriptionally distinct at fetal stageLimb fibroblasts display promiscuous identities reflecting their interconnected 3D-networkFibroblasts switch from providing positional information to differentiation during development

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Identification of Periostin as a critical niche for myofibroblast dynamics and fibrosis during tendon healing

Ackerman,

Muscat,

Adjei-Sowah

et al. 2024

Matrix Biology

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Periostin modulates extracellular matrix behavior in tendons

Cited by 7 publications

References 28 publications

Limb connective tissue is organized in a continuum of promiscuous fibroblast identities during development

Limb connective tissue is organized in a continuum of promiscuous fibroblast identities during development

Limb connective tissue is organized in a continuum of promiscuous fibroblast identities during development

Identification of Periostin as a critical niche for myofibroblast dynamics and fibrosis during tendon healing

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