Peripheral and central antinociceptive effects of the butanolic fraction of Byrsonima verbascifolia leaves on nociception-induced models in mice

Abstract

Byrsonima verbascifolia (Malpighiaceae), commonly known as 'murici', is used in folk medicine, for example, in the treatment of inflammation. The anti-inflammatory activity of the butanolic fraction of B. verbascifolia leaves (BvBF) was previously reported by our group, and the present study was designed to evaluate their antinociceptive effects. BvBF (25, 50, and 100 mg/kg) administered intraperitoneally (i.p.) inhibited acetic acid induced abdominal writhing. In the formalin test, BvBF (10, 30 and 100 mg/kg, i.p.) caused a reduction in licking time in both the neurogenic and inflammatory phases. Moreover, we demonstrated that BvBF (30 and 100 mg/kg, i.p.) caused an increase in the latency to response in the hot-plate test. These results demonstrate that BvBF possesses marked peripheral and central antinociceptive activities. Pre-treatment with the non-selective receptor antagonist naloxone (5 mg/kg, i.p.) abolished the antinociceptive effects of BvBF (100 mg/kg, i.p.) in the neurogenic phase of the formalin and hot-plate tests. The anti-inflammatory activity of BvBF (previously reported) as well as the participation of the opioidergic system seems to be responsible, at least in part, for these antinociceptive effects. Finally, BvBF at the doses investigated (25, 50 and 100 mg/Kg) did not cause any toxicity signals, showing that the antinociceptive activity is devoid of sedative and hypomotility effects.

In vivo anti-inflammatory and antinociceptive effects, and in vitro antioxidant, antiglycant and anti-neuroinflammatory actions of Syzygium malaccense

“…In the first (neurogenic) phase, up to 5 min after the formalin injection, there is a direct sensitization of C and Aδ fibers An Acad Bras Cienc (2021) 93(Suppl. 4) e20210457 14 | 19 due to the release of neurogenic mediators, including excitatory amino acids, nitric oxide, and substance P. The second (inflammatory) phase, which occurs between 15 and 30 min after formalin injection, is related to the release of several inflammatory mediators, such as prostaglandins, histamine, bradykinin, and serotonin (Hunskaar et al 1985, Saldanha et al 2017, Silva et al 2013). Thus, antinociceptive drugs that act centrally or in peripheral fibers, interfering in the central nociceptive pathways, are capable of inhibiting the licking paw time of both phases.…”

Anti-inflammatory, antinociceptive and antioxidant activities of the hydromethanolic fraction from Annona nutans leaves

“…The FHMeOH produced central antinociceptive effects, verified by the increase in reaction time. However, further studies are needed to establish the possible mechanisms of the antinociceptive action of FHMeOH (SALDANHA et al, 2016).…”

Synthesis and evaluation of pharmacological activities of some 3-O-benzyl-4-C-(hydroxymethyl)-1,2-O-isopropylidene-α-D-ribofuranose derivatives as potential anti-inflammatory agents and analgesics

“…The synthesized compounds were tested for central analgesic activity using the tail immersion method described by Saldanha et al . with some slight modifications ( 19 ). Fifty Swiss albino mice were divided in ten equal groups- one for positive control (morphine, 2 mg/kg), one for negative control (0.9% NaCl saline solution) and the others were for testing two separate doses (25 and 50 mg/kg) of the four synthesized compounds.…”

“…For testing the peripheral analgesic activity, the acetic acid-induced writhing model of was employed with slight modifications ( 19 ). Fifty Swiss albino mice were grouped similarly as in the previous test and pre-treated orally with 0.9% NaCl saline solution (as negative control), diclofenac (as positive control), and synthesized compounds (25, 50 mg/kg) 30 min before intraperitoneal injection of 0.7% acetic acid (10 mL/kg, i.p.).…”