Peripheral Blood CD4 and CD8 Double-Positive T Cells of Rhesus Macaques Become Vulnerable to Simian Immunodeficiency Virus by In Vitro Stimulation Due to the Induction of CCR5

Matsumoto, Yusuke; Miura, Tomoyuki; Akari, Hirofumi; Goto, Yoshitaka; Haga, Takeshi

doi:10.1292/jvms.09-0588

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…We also measured the proportion of CD3 + cells that expressed both CD4 and CD8. These CD4 + CD8 + double‐positive cells in the gut have been previously shown to be highly active, highly CCR5‐expressing T cells that are primary targets for SIV infection in macaques . Consistent with these findings, double‐positive T cells from upper and lower GI biopsies were significantly depleted in animal Z09087.…”

Section: Resultssupporting

confidence: 74%

Robust suppression of env‐SHIV viremia in Macaca nemestrina by 3‐drug ART is independent of timing of initiation during chronic infection

Peterson

Younan

Polacino

et al. 2013

J of Medical Primatology

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Background Nonhuman primates (NHPs) are an important model organism for studies of HIV pathogenesis and pre-clinical evaluation of anti-HIV therapies. The successful translation of NHP-derived data to clinically relevant anti-HIV studies will require better understanding of the viral strains and NHP species used, and their responses to existing antiretroviral therapies (ART). Methods Five pigtailed macaques (M. nemestrina) were productively infected with the SIV/HIV chimeric virus SHIV-1157ipd3N4 following intravenous challenge. After 8 or 27 weeks, ART (PMPA, FTC, Raltegravir) was initiated. Viral load, T-Cell counts, and production of SHIV-specific antibodies were monitored throughout the course of infection and ART. Results ART led to a rapid and sustained decrease in plasma viral load. Suppression of plasma viremia by ART was independent of the timing of initiation during chronic infection. Conclusions We present a new NHP model of HIV infection on antiretroviral therapy, which should prove applicable to multiple clinically relevant anti-HIV approaches.

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Section: Resultssupporting

confidence: 74%

Robust suppression of env‐SHIV viremia in Macaca nemestrina by 3‐drug ART is independent of timing of initiation during chronic infection

Peterson

Younan

Polacino

et al. 2013

J of Medical Primatology

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“…In humans, DP T have been described in the peripheral blood as differentiated antigen-specific cells developed in response to viral pathogens (44) and in the affected tissues of suspected autoimmune diseases (reviewed in Reference 39); DP T were also identified in BAL of HIV-infected patients with chronic obstructive pulmonary disease but not in HIV infection or chronic obstructive pulmonary disease alone (45). In both humans and macaques, peripheral blood DP T display distinct functional responses to both pathogen-specific and nonspecific stimulation (44,(46)(47)(48); the mycobacterialspecific responses of these cells have not yet been published. However, determination of the distinct antigen specificities and functional responses of this population is needed to determine whether its presence alters the overall BAL T-cell response in macaques in a way that is not recapitulated in humans.…”

Section: and Cd11cmentioning

confidence: 99%

Diversity of Human and Macaque Airway Immune Cells at Baseline and during Tuberculosis Infection

Silver

Myers

Jarvela

et al. 2016

Am J Respir Cell Mol Biol

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Immune cells of the distal airways serve as "first responders" of host immunity to the airborne pathogen Mycobacterium tuberculosis (Mtb). Mtb infection of cynomolgus macaques recapitulates the range of human outcomes from clinically silent latent tuberculosis infection (LTBI) to active tuberculosis of various degrees of severity. To further advance the application of this model to human studies, we compared profiles of bronchoalveolar lavage (BAL) cells of humans and cynomolgus macaques before and after Mtb infection. A simple gating strategy effectively defined BAL T-cell and phagocyte populations in both species. BAL from Mtb-naive humans and macaques showed similar differential cell counts. BAL T cells of macaques were composed of fewer CD41 cells but more CD81 and CD4 1 CD8 1 double-positive cells than were BAL T cells of humans. The most common mononuclear phagocyte population in BAL of both species displayed coexpression of HLA-DR, CD206, CD11b, and CD11c; however, multiple phagocyte subsets displaying only some of these markers were observed as well. Macaques with LTBI displayed a marked BAL lymphocytosis that was not observed in humans with LTBI. In macaques, the prevalence of specific mononuclear phagocyte subsets in baseline BAL correlated with ultimate outcomes of Mtb infection (i.e., LTBI versus active disease).Overall, these findings demonstrate the comparability of studies of pulmonary immunity to Mtb in humans and macaques. They also indicate a previously undescribed complexity of airway mononuclear phagocyte populations that suggests further lines of investigation relevant to understanding the mechanisms of both protection from and susceptibility to the development of active tuberculosis within the lung.

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Peripheral Blood CD4 and CD8 Double-Positive T Cells of Rhesus Macaques Become Vulnerable to Simian Immunodeficiency Virus by In Vitro Stimulation Due to the Induction of CCR5

Cited by 2 publications

References 17 publications

Robust suppression of env‐SHIV viremia in Macaca nemestrina by 3‐drug ART is independent of timing of initiation during chronic infection

Robust suppression of env‐SHIV viremia in Macaca nemestrina by 3‐drug ART is independent of timing of initiation during chronic infection

Diversity of Human and Macaque Airway Immune Cells at Baseline and during Tuberculosis Infection

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