Peripheral blood miRNAs as a biomarker for chronic cardiovascular diseases

Abstract

Vascular diseases are the most prevalent diseases worldwide. This study intended to analyze peripheral blood miRNA levels and their correlation with NT-pro-BNP and cTN-I in patients with atherosclerosis or pre-atherosclerotic conditions to build a dynamic correlation between vascular diseases and their biomarkers. Serum NT-pro-BNP and cTN-I levels were measured by their respective ELISA kits. The miRNA levels were assayed by quantitative PCR. Unique miRNA signatures were identified for both atherosclerosis and pre-atherosclerosis. The levels of miR-92a, 126, 130a, 222, and 370 levels were decreased in the peripheral blood of pre-atherosclerotic subjects. In atherosclerosis, miR-21, 122, 130a, and 211 were significantly increased whereas miR-92a, 126, and 222 were markedly decreased. Serum levels of NT-pro-BNP and cTN-I correlated with each other and increased with the progression of atherosclerosis. Moreover, the levels of cTN-I and NT-pro-BNP were positively correlated with miR-21 and negatively correlated with miR-126. Integrating specific pattern of miRNA levels with NT-pro-BNP and/or cardiac troponin may improve the diagnosis of cardiovascular diseases.

Decreased expression of circulating microRNA-126 in patients with type 2 diabetic nephropathy: A potential blood-based biomarker

“…miR-126 also protects against atherosclerosis by inhibiting VCAM-1 during inflammation (42,43) and contributes to renal microvascular heterogeneity of VCAM-1 protein expression in acute inflammation (44). Diabetes-induced endothelial dysfunction has been proposed as a major mechanism implicated in the pathogenesis and development of DN, occurring through activation of signal transducers of metabolic, hemodynamic and inflammatory factors (3,4), which modifies the function and morphology of neighboring cells, triggering a cascade of inflammatory, proliferative and profibrotic responses in progressive DN (4).…”

MicroRNAs Regulate Cytokine Responses in Gingival Epithelial Cells

“…Instead, our results indicate that miR-155 is an NF-B transactivational target that is involved in a negative-feedback loop through downregulation of IKKs and possible other genes. Lastly, miR-210 is strongly linked to hypoxia pathways (47,48) that are critically important in host defenses during the course of bacterial infections (49). Although miR-210 was reported to negatively regulate production of proinflammatory cytokines in lipopolysaccharide (LPS)-stimulated murine macrophages through NF-B targeting (50), we did not observe any significant alteration of NF-B1 mRNA levels in miR-210 KD cells infected with P. gingivalis for 6 h, despite the observed increased cytokine induction.…”

MiR-92a – a key player in cardiovascular remodeling

“…Interestingly, circulating levels of miR-92a are significantly downregulated in patients with coronary artery disease, which might represent a compensatory mechanism. Selective analysis of these patients for vasculoprotective drug therapies revealed a trend towards lower levels of miR-92a in patients with statin therapy compared with patients without statin therapy [16,17] . Thus, low circulating levels of miR-92a likely represent a compensatory protective mechanism that might be boosted in response to statin therapy.…”