Peripheral blood mononuclear cell hyperresponsiveness in patients with premature myocardial infarction without traditional risk factors

Mol, Jan-Quinten; Tuijl, Julia van; Bekkering, Siroon; Heijden, Charlotte D C C van der; Damen, Sander; Cossins, Benjamin C.; Emst, Liesbeth van; Nielen, Tim Mj; Rodwell, Laura; Li, Yang; Pop, Gheorghe; Netea, Mihai G.; Royen, Niels van; Riksen, Niels P.; Messaoudi, Saloua El

doi:10.1016/j.isci.2023.107183

Cited by 3 publications

(4 citation statements)

References 48 publications

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“…In fact, previous studies using bulk RNA sequencing have failed to find a robust immune signature after MI because the contribution of both proinflammatory and anti-inflammatory immune subsets to tissue repair may be under-detected when analyzed in bulk due to the averaging of transcription profiles from these diverse immune cell subsets. 7 Using single-cell RNA sequencing together with plasma proteomics, van Blokland et al 4 performed the first unbiased and comprehensive survey of immune signatures at the single-cell level on peripheral blood mononuclear cells collected at the time of STEMI as well as 24 hours and 4 to 6 weeks post-STEMI. The longitudinal immune signatures of patients with STEMI are then compared with the immune profile of an external group of 38 sex-and age-matched controls.…”

Section: See Article By Van Blokland Oelen and Groot Et Almentioning

confidence: 99%

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Immune Cellular Dynamics in the Peripheral Blood: A Barometer for Cardiovascular Risk?

Yarahmadi,

Nguyen

2024

Circ: Genomic and Precision Medicine

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Section: See Article By Van Blokland Oelen and Groot Et Almentioning

confidence: 99%

mentioning

confidence: 99%

Immune Cellular Dynamics in the Peripheral Blood: A Barometer for Cardiovascular Risk?

Yarahmadi,

Nguyen

2024

Circ: Genomic and Precision Medicine

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“…The isolated monocytes from patients with SMuRFless MI were characterized by increased cytokine production capacity. 3 Statins, besides lipid‐lowering capacities, also have anti‐inflammatory effects. We now hypothesize that the beneficial effect of statins in patients with SMuRFless MI is, at least in part, attributable to anti‐inflammatory effects.…”

mentioning

confidence: 99%

“…To explore this, we assessed in detail the effect of 2‐week interruption of statin treatment on monocyte phenotype in the patients with SMuRFless MI included in that study. 3 …”

mentioning

confidence: 99%

Two‐Week Interruption of Statin Therapy Results in an Exaggerated Inflammatory Monocyte Phenotype in Young Patients With Myocardial Infarction Without Standard Modifiable Risk Factors

Mol,

van Tuijl,

Bekkering

et al. 2024

JAHA

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The alpha 7 nicotinic acetylcholine receptor agonist PHA 568487 dampens inflammation in PBMCs from patients with newly discovered coronary artery disease

Mjörnstedt,

Wilhelmsson,

Ulleryd

et al. 2024

American Journal of Physiology-Heart and Circulatory Physiology

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The alpha7 nicotinic acetylcholine receptor (α7nAChR) regulates inflammation in experimental models and is expressed in human peripheral blood mononuclear cells (PBMCs) and in human atherosclerotic plaques. However, its role in regulating inflammation in patients with cardiovascular disease is unknown. This study aims to investigate whether α7nAChR stimulation can reduce the inflammatory response in PBMCs from patients with newly diagnosed coronary artery disease (CAD). Human PBMCs, extracted from patients with verified CAD (n=38) and control participants with healthy vessels (n=38), were challenged in vitro with lipopolysaccharide (LPS) in combination with the α7nAChR agonist PHA 568487. Cytokine levels of the supernatants were analyzed using a multiplex immunoassay. Patients in the CAD group were re-examined after 6 months. The immune response to LPS did not differ between PBMCs from control group and CAD patients. α7nAChR stimulation decreased TNFα in both control participants and CAD patients. The most pronounced effect of α7nAChR stimulation was observed in CAD patients at their first visit, where 15 of 17 cytokines were decreased (IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p70), IL-17A, G-CSF, GM-CSF, IFN-γ, MCP-1, MIP-1β and TNFα). In conclusion, stimulation with α7nAChR agonist PHA 568487 dampens the inflammatory response in human PBMCs. This finding suggests that the anti-inflammatory properties of the α7nAChR may have a role in treating CAD.

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Peripheral blood mononuclear cell hyperresponsiveness in patients with premature myocardial infarction without traditional risk factors

Cited by 3 publications

References 48 publications

Immune Cellular Dynamics in the Peripheral Blood: A Barometer for Cardiovascular Risk?

Immune Cellular Dynamics in the Peripheral Blood: A Barometer for Cardiovascular Risk?

Two‐Week Interruption of Statin Therapy Results in an Exaggerated Inflammatory Monocyte Phenotype in Young Patients With Myocardial Infarction Without Standard Modifiable Risk Factors

The alpha 7 nicotinic acetylcholine receptor agonist PHA 568487 dampens inflammation in PBMCs from patients with newly discovered coronary artery disease

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