Peripheral blood mononuclear cell‐mediated cytolytic activity during cytomegalovirus (CMV) infection of guinea pigs

Abstract

Inbred Strain-2 guinea pigs exhibited endogenous peripheral blood mononuclear cell (PBMC)-mediated cytolytic activity against xenogeneic MA104 targets and guinea pig cytomegalovirus (gpCMV)-infected syngeneic and allogeneic targets. This endogenous cytolysis was unaffected by monoclonal T-cell antibody depletion but was diminished by removal of plastic adherent cells. In nonadherent effector populations, cytolysis was mediated predominately by large granular lymphocytes (LGL). During gpCMV infection, cytolysis of both target types was augmented (MA 104 for 3 weeks and gpCMV targets for 10-14 weeks). Augmented cytolysis of gpCMV targets was MHC-unrestricted and was diminished by removal of plastic adherent cells or monoclonal antibody depletion of T-cells, being found largely in LGL enriched populations. A role for this augmented activity in limiting gpCMV infection in inbred guinea pigs is suggested by the temporal association of augmented cytolysis of gpCMV targets with the cessation of viremia and clinical recovery.

Modification of Maternal and Congenital Cytomegalovirus Infection by Anti–Glycoprotein B Antibody Transfer in Guinea Pigs

“…Minced explant cultures of a -cm piece of each pla-0.5 ϫ 0.5 ϫ 0.5 centa or pup tissue were cocultivated on GPL monolayers also incubated for 4 weeks [13]. GPCMV isolates were identified by characteristic cytopathic effect in GPL cells, and selective isolates were confirmed by neutralization with anti-GPCMV antibody [14].…”

“…on GPL monolayers incubated for 4 weeks after inoculation [14]. Minced explant cultures of a -cm piece of each pla-0.5 ϫ 0.5 ϫ 0.5 centa or pup tissue were cocultivated on GPL monolayers also incubated for 4 weeks [13].…”

“…http://jid.oxfordjournals.org/ Downloaded from a Blood cultures of whole blood were done on guinea pig monolayers, as described elsewhere [14]. b Of the original 7 animals, 2 were killed on day 7 and 2 on day 11; 1 animal died on day 8. c Pregnancy loss was defined as abortion, premature delivery, or fetal resorption.…”

Preconception Vaccination with a Glycoprotein B (gB) DNA Vaccine Protects against Cytomegalovirus (CMV) Transmission in the Guinea Pig Model of Congenital CMV Infection

“…22122; ATC VR682) was propagated on guinea pig fibroblast lung cells (GPL; ATCC CCL 158) and was maintained in F-12 medium supplemented with 10% fetal calf serum (HyClone Laboratories), 10,000 IU/ L penicillin, 10 mg/L streptomycin (Gibco-BRL), and 7.5% NaHCO 3 (Gibco-BRL). Salivary gland-passaged stocks (SG virus) were prepared by sequential passage in strain-2 guinea pigs, as described elsewhere [30].…”

Posttherapy suppression of genital herpes simplex virus (HSV) recurrences and enhancement of HSV-specific T-cell memory by imiquimod in guinea pigs

“…Major histocompatibility complex (MHC) unrestricted cytolytic activity against HSV-infected HFF targets was assayed (in the 5-day experiment only) by standard 51Cr release assays as previously described (9,10 14 after inoculation) were also observed in the randomized placebo and imiquimod groups for both the 5-and 21-day treatment studies (data not shown).…”