Peripheral CD4+/CD8+ T-lymphocyte counts estimated by an immunocapture method in the normal healthy south Indian adults and HIV seropositive individuals

Kannangai, Rajesh; Prakash, Kotha Jaya; Ramalingam, Sandeep; Abraham, O C; Mathews, K.P; Mv, Jesudason; Sridharan, Gopalan

doi:10.1016/s1386-6532(00)00080-9

Cited by 25 publications

(16 citation statements)

References 5 publications

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“…Flow cytometry, the standard method for the estimation of CD4 ϩ and CD8 ϩ T-cell counts, is available only in a very few centers in developing countries. An earlier study showed that the Capcellia method can be used as an alternative to flow cytometry for the quantitation of CD4 ϩ and CD8 ϩ T-cell counts in less well-equipped laboratories (9). The study showed a significant difference in CD4 ϩ and CD8 ϩ T-cell counts and the ratio measured by Capcellia between asymptomatic and symptomatic HIV-infected individuals.…”

Section: The Correlation Between the Cd4mentioning

confidence: 99%

“…Hence, it is important to have cost-effective monitoring tests. The technique of estimating CD4 ϩ and CD8 ϩ T-cell counts by an immunocapture method (Capcellia) was investigated earlier (9). In the present study, we investigated the correlation of CD4 ϩ T-cell counts as estimated by the immunocapture method with HIV-1 load estimation and the performance of this assay in relation to flow cytometry.…”

mentioning

confidence: 98%

“…The Capcellia CD4/CD8 whole-blood kit from BioRad (previously manufactured and marketed by Sanofi Diagnostic Pasteur, Marnes-la-Coquette, France) is an immunocapture assay based on an enzyme-linked immunosorbent assay (ELISA); paramagnetic particles coated with anti-pan-T-cell antibodies (anti-CD2) are used to capture T lymphocytes (1,9). The unbound cells are washed using a manual washing manifold connected to an electric vacuum pump.…”

mentioning

confidence: 99%

“…The T-cell subsets are estimated using peroxidase-labeled monoclonal antibodies specific for CD4 ϩ and CD8 ϩ T cells. The mean coefficients of variation for CD4 ϩ and CD8 ϩ T-cell counts were 6.5 and 12%, respectively, for samples obtained from healthy controls and tested in duplicate (9). Hence, although the kit manufacturer recommended that each sample be tested once, we tested all the samples in duplicate wells and took the average cell counts as CD4 ϩ and CD8 ϩ T-cell counts per microliter.…”

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confidence: 99%

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Correlation of CD4 ⁺ T-Cell Counts Estimated by an Immunocapture Technique (Capcellia) with Viral Loads in Human Immunodeficiency Virus-Seropositive Individuals

Kannangai¹,

Ramalingam²,

et al. 2001

Clin Diagn Lab Immunol

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As antiretroviral therapy becomes more affordable, valid, reliable, and inexpensive laboratory tests are also needed to monitor the progression of disease in people with human immunodeficiency virus (HIV) infection. The CD4؉ T-cell counts estimated by Capcellia, an immunocapture method, and flow cytometry were compared and were correlated with HIV type 1 (HIV-1) load. There was a significant negative correlation between the HIV-1 load and CD4 ؉ T-cell counts estimated by flow cytometry (r ‫؍‬ ؊0.63, P ‫؍‬ <0.001) as well as between the HIV-1 load and CD4؉ T-cell counts estimated by Capcellia (r ‫؍‬ ؊0.61, P ‫؍‬ <0.001). Capcellia is a cost-effective, user-friendly assay that correlated well with HIV-1 load determinations for individuals both with and without treatment.

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Section: The Correlation Between the Cd4mentioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Correlation of CD4 ⁺ T-Cell Counts Estimated by an Immunocapture Technique (Capcellia) with Viral Loads in Human Immunodeficiency Virus-Seropositive Individuals

Kannangai¹,

Ramalingam²,

et al. 2001

Clin Diagn Lab Immunol

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“…They are involved in activating and directing B lymphocyte cells. The lymphocyte subpopulation CD4/CD8 ratios and levels of immunoglobulins are considered as diagnostic markers for various disease conditions [5,6]. Measurement of immunoglobulin levels and CD4/CD8 ratios is of immense value in assessing the immune status of children in relation to a variety of conditions of nutritional imbalance in diseases [7,8].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of a Fish Protein Hydrolysate in Malnourished Children

Nesse¹,

Nagalakshmi

Marimuthu

et al. 2011

Ind J Clin Biochem

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Protein hydrolysates are good nutritional supplements as their bioactive ingredients can be easily absorbed and utilized for various metabolic activities. A fish protein hydrolysate (Amizate), prepared by a unique process of hydrolysis has the advantage of high di/tri peptide content (\10 kDa) along with essential and non essential amino acids, micronutrients and vitamins. The effect of Amizate on malnourished children (6-8 years, a total of 438) of Grade I and II (Gomez's classification) with respect to immunoglobulins, CD4/CD8 ratios and hemoglobin was examined. Measurement of these parameters during the user trial study (at the beginning and the end after 4 months) indicated that the levels of the immunological parameters were not significantly altered by the Amizate treatment. The values of immunoglobulins and CD4/CD8 ratios of malnourished children (India) are in the normal range and are in accordance with the reported values of various ethnic groups.

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Normal values of CD4 and CD8 lymphocyte subsets in healthy indian adults and the effects of sex, age, ethnicity, and smoking

Uppal

Verma

Dhot

2003

Cytometry Part B Clinical

173

142

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When compared with other published series, the CD4 and CD8 values in healthy Indians were no different from those reported in the West. These observations have important clinical implications for the use of T-lymphocyte subset measurements in India, especially in the management of HIV infection. The normal ranges established by this study can be used as a reference for decisions made in clinical practice.

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Peripheral CD4+/CD8+ T-lymphocyte counts estimated by an immunocapture method in the normal healthy south Indian adults and HIV seropositive individuals

Cited by 25 publications

References 5 publications

Correlation of CD4 ⁺ T-Cell Counts Estimated by an Immunocapture Technique (Capcellia) with Viral Loads in Human Immunodeficiency Virus-Seropositive Individuals

Correlation of CD4 ⁺ T-Cell Counts Estimated by an Immunocapture Technique (Capcellia) with Viral Loads in Human Immunodeficiency Virus-Seropositive Individuals

Efficacy of a Fish Protein Hydrolysate in Malnourished Children

Normal values of CD4 and CD8 lymphocyte subsets in healthy indian adults and the effects of sex, age, ethnicity, and smoking

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Peripheral CD4+/CD8+ T-lymphocyte counts estimated by an immunocapture method in the normal healthy south Indian adults and HIV seropositive individuals

Cited by 25 publications

References 5 publications

Correlation of CD4 + T-Cell Counts Estimated by an Immunocapture Technique (Capcellia) with Viral Loads in Human Immunodeficiency Virus-Seropositive Individuals

Correlation of CD4 + T-Cell Counts Estimated by an Immunocapture Technique (Capcellia) with Viral Loads in Human Immunodeficiency Virus-Seropositive Individuals

Efficacy of a Fish Protein Hydrolysate in Malnourished Children

Normal values of CD4 and CD8 lymphocyte subsets in healthy indian adults and the effects of sex, age, ethnicity, and smoking

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Product

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Correlation of CD4 ⁺ T-Cell Counts Estimated by an Immunocapture Technique (Capcellia) with Viral Loads in Human Immunodeficiency Virus-Seropositive Individuals

Correlation of CD4 ⁺ T-Cell Counts Estimated by an Immunocapture Technique (Capcellia) with Viral Loads in Human Immunodeficiency Virus-Seropositive Individuals