Peripheral Coding of Sex Pheromone Blends with Reverse Ratios in Two Helicoverpa Species

Wu, Han; Hou, Chao; Huang, Ling-Qiao; Fang, Yan; Wang, Chen-Zhu

doi:10.1371/journal.pone.0070078

Cited by 39 publications

(58 citation statements)

References 63 publications

(91 reference statements)

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“…Preparation of moths and optical recording were performed as described previously (Wu et al, 2013). Briefly, virgin male moths were restrained in plastic tubes and fixed with dental wax.…”

Section: Methodsmentioning

confidence: 99%

“…They use the same compounds, (Z)-11-hexadecenal (Z11-16:Ald) and (Z)-9-hexadecenal (Z9-16:Ald) as their main sex pheromone constituents but in almost reversed ratios, 98:2 and 5:95, respectively (Kehat et al, 1980; Cork et al, 1992; Wang et al, 2005). Accordingly, the population ratios of the OSNs of A, C type sensilla, responsive to Z11-16:Ald and Z9-16:Ald, respectively, are reversed in the two species (Berg and Mustaparta, 1995; Wu et al, 2013; Berg et al, 2014; Xu et al, 2016). In H. assulta , the C type sensilla species house two responding neurons.…”

Section: Introductionmentioning

confidence: 95%

“…In H. armigera , the two neurons in C type sensilla are difficult to differentiate due to their similar spike amplitudes (Wu et al, 2015; Xu et al, 2016). As in other Heliothine species (Christensen et al, 1991; Berg et al, 1998; Vickers and Christensen, 2003), the pheromonal information received by OSNs is conveyed to the MGC area of the AL (Berg et al, 2005; Zhao and Berg, 2010; Wu et al, 2013, 2015). In H. armigera , the major sex pheromone component Z11-16:Ald elicits a response in the larger glomerulus of the MGC, the cumulus, while the minor component Z9-16:Ald activates one smaller glomerulus of MGC, the posterior dorsomedial unit (Dm-p) (Wu et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…As in other Heliothine species (Christensen et al, 1991; Berg et al, 1998; Vickers and Christensen, 2003), the pheromonal information received by OSNs is conveyed to the MGC area of the AL (Berg et al, 2005; Zhao and Berg, 2010; Wu et al, 2013, 2015). In H. armigera , the major sex pheromone component Z11-16:Ald elicits a response in the larger glomerulus of the MGC, the cumulus, while the minor component Z9-16:Ald activates one smaller glomerulus of MGC, the posterior dorsomedial unit (Dm-p) (Wu et al, 2013). Conversely, in H. assulta , the cumulus of the AL receives inputs from OSNs tuned to Z9-16:Ald, while the smaller glomerulus, ventral unit, is connected to OSNs tuned to Z11-16:Ald (Berg et al, 2005; Wu et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…In H. armigera , the major sex pheromone component Z11-16:Ald elicits a response in the larger glomerulus of the MGC, the cumulus, while the minor component Z9-16:Ald activates one smaller glomerulus of MGC, the posterior dorsomedial unit (Dm-p) (Wu et al, 2013). Conversely, in H. assulta , the cumulus of the AL receives inputs from OSNs tuned to Z9-16:Ald, while the smaller glomerulus, ventral unit, is connected to OSNs tuned to Z11-16:Ald (Berg et al, 2005; Wu et al, 2013). …”

Section: Introductionmentioning

confidence: 99%

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The Inheritance of the Pheromone Sensory System in Two Helicoverpa Species: Dominance of H. armigera and Possible Introgression from H. assulta

Dong

et al. 2017

Front. Cell. Neurosci.

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Hybridization of sympatric closely related species may sometimes lead to introgression and speciation. The sister species Helicoverpa armigera and Helicoverpa assulta both use (Z)-11-hexadecenal and (Z)-9-hexadecenal as sex pheromone components but in reversed ratios. Female H. armigera and male H. assulta could hybridize and produce fertile male hybrids, which can then backcross with females of the two parent species to get backcross lines in the laboratory. In this study, we compared the olfactory responses to pheromone compounds in the periphery and in the antennal lobes (ALs) of males of the two species, as well as of their hybrids and backcrosses. Single-sensillum recordings were carried out to explore characteristics of male-specific sensilla on the antennae, and in vivo calcium imaging combined with digital 3D-reconstruction was used to describe what happens in the macroglomerular complex (MGC) of the AL. The results show that the population ratio of the two male-specific types of olfactory sensory neurons responding to two sex pheromone components are controlled by a major gene, and that the allele of H. armigera is dominant. Consistently, the study of the representative areas activated by sex pheromone components in the ALs further support the dominance of H. armigera. However, the topological structure of the MGC in the hybrid was similar but not identical to that in H. armigera. All subtypes of male-specific sensilla identified in the two species were found in the male hybrids and backcrosses. Moreover, two new subtypes with broader response spectra (the expanded A subtype and the expanded C subtype) emerged in the hybrids. Based on the inheritance pattern of the pheromone sensory system, we predict that when hybridization of female H. armigera and male H. assulta occurs in the field, male hybrids would readily backcross with female H. armigera, and introgression might occur from H. assulta into H. armigera through repeated backcrossing.

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“…Preparation of moths and optical recording were performed as described previously (Wu et al, 2013). Briefly, virgin male moths were restrained in plastic tubes and fixed with dental wax.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Inheritance of the Pheromone Sensory System in Two Helicoverpa Species: Dominance of H. armigera and Possible Introgression from H. assulta

Dong

et al. 2017

Front. Cell. Neurosci.

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Comparison of male antennal morphology and sensilla physiology for sex pheromone olfactory sensing between sibling moth species: Ectropis grisescens and Ectropis obliqua (Geometridae)

Liu

Luo

et al. 2019

Arch Insect Biochem Physiol

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Ectropis grisescens and Ectropis obliqua (Lepidoptera: Geometridae) are sibling pest species that co-occur on tea plants.The sex pheromone components of both species contain (Z,Z,Z)-3,6,9-octadecatriene and (Z,Z)-3,9-cis-6,7-epoxy-octadecadiene. E. obliqua has (Z,Z)-3,9-cis-6,7-epoxy-nonadecadiene as an additional sex pheromone component, which ensures reproductive segregation between the two species.To ascertain the detection mechanism of olfactory organs for sex pheromone components of E. grisescens and E. obliqua, we applied scanning electron microscopy and single sensillum recording to compare antennal morphology and sensillum physiology in the two species. There was no apparent morphological difference between the antennae of the two species. Both species responded similarly to all three sex pheromone components, including, E. obliqua specific component. The distribution patterns of antennal sensilla trichodea differed between the two species. Sex pheromone olfactory sensing in these sibling species appears to be determined by the density of different types of olfactory sensing neurons. Dose-dependent responses of sensilla trichodea type 1 to (Z,Z)-3,9-cis-6,7-epoxy-octadecadiene, the most abundant component, showed an "all or none" pattern and the other two components showed Arch. Insect Biochem. Physiol. 2019;101:e21545.wileyonlinelibrary.com/journal/arch

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Optimization of a pheromone lure by analyzing the peripheral coding of sex pheromones of Spodoptera frugiperda in China

Wang

Zhang

Guo

et al. 2022

Pest Management Science

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BACKGOUND Sex pheromones of the fall armyworm, Spodoptera frugiperda, show differences in composition and proportions in different geographical populations, but always contain Z9‐14:OAc as the major component. Odorant receptor neurons (ORNs) housed in the long trichoid sensilla (TS) of male antennae are essential to detect female‐released sex pheromones in moths. RESULTS In this study, we identified seven components from pheromone gland extracts of female S. frugiperda in the Yunnan population from China, including (Z)‐7‐dodecen‐1‐yl acetate (Z7‐12:OAc), (Z)‐9‐tetradecenal (Z9‐14:Ald), (Z)‐9‐dodecen‐1‐yl acetate (Z9‐12:OAc), (Z)‐9‐tetradecen‐1‐yl acetate (Z9‐14:OAc), (E)‐11‐tetradecen‐1‐yl acetate (E11‐14:OAc), (Z)‐11‐tetradecen‐1‐yl acetate (Z11‐14:OAc) and (Z)‐11‐hexadecen‐1‐yl acetate (Z11‐16:OAc) at a ratio of 1.2:4:0.8:79.1:1.6:1.6:11.7 by gas chromatography coupled with mass spectrometry. Gas chromatography‐electroantennographic detection showed that Z9‐14:OAc, Z7‐12:OAc and Z11‐16:OAc are the male antennal active components. Peripheral coding of pheromones in males was investigated by single sensillum recording. Five functional neurons housed in three types of TS were identified based on profiles of neuronal responses, which are responsible for attractive component Z9‐14:OAc, synergistic components Z7‐12:OAc, Z11‐16:OAc, interspecific pheromones (Z)‐9‐tetradecen‐1‐ol (Z9‐14:OH) and (Z,E)‐9,12‐tetradecadien‐1‐yl acetate (Z9,E12‐14:OAc), respectively. Wind tunnel and field tests demonstrated that a ternary combination of Z9‐14:OAc, Z7‐12:OAc and Z11‐16:OAc at a ratio of 88:1:11 shows the strongest attractiveness to males. Conclusion An optimized pheromone blend of Z9‐14:OAc, Z7‐12:OAc and Z11‐16:OAc in an 88:1:11 ratio was identified for monitoring the invasive pest S. frugiperda in China. Five functional ORNs encoding intra‐ and interspecific pheromones were identified in male antennae, of which three neurons encode attractive component Z9‐14:OAc, synergistic components Z7‐12:OAc and Z11‐16:OAc, respectively, and the other two neurons encode interspecific pheromones Z9‐14:OH and Z9,E12‐14:OAc, separately. © 2022 Society of Chemical Industry.

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Peripheral Coding of Sex Pheromone Blends with Reverse Ratios in Two Helicoverpa Species

Cited by 39 publications

References 63 publications

The Inheritance of the Pheromone Sensory System in Two Helicoverpa Species: Dominance of H. armigera and Possible Introgression from H. assulta

The Inheritance of the Pheromone Sensory System in Two Helicoverpa Species: Dominance of H. armigera and Possible Introgression from H. assulta

Comparison of male antennal morphology and sensilla physiology for sex pheromone olfactory sensing between sibling moth species: Ectropis grisescens and Ectropis obliqua (Geometridae)

Optimization of a pheromone lure by analyzing the peripheral coding of sex pheromones of Spodoptera frugiperda in China

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