Peripheral GABA receptors regulate colonic afferent excitability and visceral nociception

Abstract

Key pointsr While the presence of GABA receptors on primary afferents has been well described, most functional analyses have focused on the regulation of transmitter release from central terminals and/or signalling in the sensory neuron cell body.r Evidence that GABA receptors are transported to peripheral terminals and that there are several sources of GABA in the colon raise the possibility that GABA signalling in the periphery may influence colonic afferent excitability.r GABA A and GABA B are present and functional in the colon, where exogenous agonists decrease the excitability of colonic afferents and suppress visceral nociception.r Endogenous GABA release within the colon is sufficient to establish the resting excitability of colonic afferents as well as the behavioural response to noxious stimulation of the colon, primarily via GABA A receptors.r Peripheral GABA receptors may serve as a viable target for the treatment of visceral pain.Abstract It is well established that GABA receptors at the central terminals of primary afferent fibres regulate afferent input to the superficial dorsal horn. However, the extent to which peripheral GABA signalling may also regulate afferent input remains to be determined. The colon was used to explore this issue because of the numerous endogenous sources of GABA that have been described in this tissue. The influence of GABA signalling on colonic afferent excitability was assessed in an ex vivo mouse colorectum pelvic nerve preparation where test compounds were applied to the receptive field. The visceromotor response (VMR) evoked by noxious colorectal distension was used to assess the impact of GABA signalling on visceral nociception, where test compounds were applied directly to the colon. Application of either GABA A or GABA B receptor agonists attenuated the colonic afferent response to colon stretch. Conversely, GABA A and GABA B receptor antagonists increased the stretch response. However, while the noxious distension-induced VMR was attenuated in the presence of GABA A and GABA B receptor agonists, the VMR was only consistently increased by GABA A receptor antagonists. These results suggest Emanuel Loeza-Alcocer received his PhD in neurobiology in the laboratory of Professor Rodolfo Delgado-Lezama in Mexico City. His research was focused on characterizing the GABAergic mechanisms underlying the presynaptic inhibition of sensory neurons. At present, he investigates peripheral GABAergic inhibitory mechanisms in the colon in the group of Michael Gold PhD at University of Pittsburgh, USA. His long-term goal is to understand how peripheral GABAergic inhibition modifies sensory perception in health and disease. E. Loeza-Alcocer and others J Physiol 597.13that GABA receptors are present and functional in the peripheral terminals of colonic afferents and activation of these receptors via endogenous GABA release contributes to the establishment of colonic afferent excitability and visceral nociception. These results suggest that increasing peripheral GABA receptor signalling ...

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