Peripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies

Golovynska, Iuliia; Берегова, Тетяна Володимирівна; Falalyeyeva, Tetyana; Stepanova, L.І.; Golovynskyi, Sergii; Qu, Junle; Ohulchanskyy, Tymish Y.

doi:10.1038/s41598-018-25753-6

Cited by 14 publications

(5 citation statements)

References 46 publications

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“…In addition, it is reasonable to ascribe such protective activity to a modulation of the NR2A and NR2B subunit expression. These findings are in agreement with the results by Golovynska et al ( 36 ) and Tsai et al ( 11 ), supporting the idea of a role of NMDA receptors in the protection of gastric mucosa.…”

Section: Discussionsupporting

confidence: 93%

Protective Effects of Borago officinalis (Borago) on Cold Restraint Stress-Induced Gastric Ulcers in Rats: A Pilot Study

et al. 2020

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Stress is a typical body's natural defense to a generic physical or psychic change. A specific linking mechanism between ulcer onset and psycho-physical stress prolonged exposure has been reported. We decided to investigate the possible effects of Borago officinalis L. (Borago) in preventing physical (stress)-induced gastric ulcers in a rat model. Eighty male Sprague-Dawley rats were randomly divided into 16 groups, pretreated with a control solution, omeprazole (20 mg/kg), Borago methanolic extract (25, 50, 100, 250, and 500 mg/kg), Borago organic extract (50, 100, 250, and 500 mg/kg), Borago aqueous extract (5, 10, 20, 30, and 40 mg/kg), and D(-)-2-Amino-5-phosphonovaleric acid (AP5) (25 mg/kg) and kept in stressful conditions such as water immersion and restraint-induced stress ulcers. The animals were sacrificed and their stomach scored for the severity and the number of gastric ulcers. Methanolic extract (500 mg/kg) significantly reduced both ulcer parameters ( *** p < 0.001 and ** p < 0.01, respectively). Aqueous and organic extract significantly decreased severity score at 5 and 10 mg/kg ( ** p < 0.01 and *** p < 0.001, respectively), and at 250 and 500 mg/kg ( *** p < 0.001), respectively, while gastric ulcers' resulted number significantly reduced only at 10 mg/kg ( * p < 0.05) and at 500 mg/kg ( ** p < 0.01), respectively. On the other hand, aqueous extract significantly increased the mucosal gastric content of cAMP ( * p < 0.05) and NR2A and NR2B subunits ( * p < 0.05 and ** p < 0.01, respectively) at 5 mg/kg. Organic extract showed also a significant cytotoxic effect at 500 and 1,000 mg/kg with a 3T3 cell viability reduction of 43.6% ( ** p < 0.01) and 92.1% ( *** p < 0.001), respectively. Borago aqueous extract at 10 mg/kg could be considered as a potential protective agent against stress-induced ulcers, and it is reasonable to possibly ascribe such protective activity to a modulation of the NR2A and NR2B subunit expression.

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Section: Discussionsupporting

confidence: 93%

Protective Effects of Borago officinalis (Borago) on Cold Restraint Stress-Induced Gastric Ulcers in Rats: A Pilot Study

et al. 2020

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“…GluRs are divided into (a) ionotropic GluRs, which include N ‐methyl‐D‐aspartate receptors (NMDARs), a‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazole‐propionate receptors (AMPARs), kainite receptors, and (b) metabotropic GluRs associated with G proteins. In the last decades, GluRs were found in cells of internal organs and immune system (Ganor & Levite, ; I. Golovynska et al, ).…”

Section: Introductionmentioning

confidence: 99%

Red and near‐infrared light induces intracellular Ca²⁺ flux via the activation of glutamate N‐methyl‐D‐aspartate receptors

Golovynska

Golovynskyi

Stepanov

et al. 2019

Journal Cellular Physiology

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Red and near‐infrared (NIR) light effect on Ca2+ ions flux through the influence on N‐methyl‐D‐aspartate receptors (NMDARs) and their functioning in HeLa cells was studied in vitro. Cells were irradiated by 650 and 808 nm laser light at different power densities and doses and the obtained effect was compared with that caused by the pharmacological agents. The laser light was found to elevate Ca2+ influx into cell cytoplasm in a dose‐dependent manner without changes of the NMDAR functioning. Furthermore, the light of both wavelengths demonstrated the ability to elevate Ca2+ influx under the pharmacological blockade of NMDARs and also might partially abolish the blockade enhancing Ca2+ influx after selective stimulation of the receptors with NMDA. Simultaneously, the light at moderate doses demonstrated a photobiostimulating effect on cells. Based on our experiments and data reported in the literature, we suggest that the low‐power visible and NIR light can instigate a cell membrane depolarization via nonthermal activation, resulting in the fast induction of Ca2+ influx into cells. The obtained results also demonstrate that NIR light can be used for nonthermal and nonpharmacological stimulation of NMDARs in cancer cells.

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“…Previous studies on how glutamate receptors in the CNS contribute to controlling gastrointestinal motility have shown that ionotropic glutamate receptors are involved in regulating the gastrointestinal tract. On the other hand, metabotropic glutamate receptors are not involved in the regulation of the gastrointestinal tract Golovynska et al (2018) . Previous studies strongly suggest that inhibition of gastric motility by NMDA or excitatory amino acids is achieved through central action Shinozaki et al (1990) .…”

Section: Discussionmentioning

confidence: 99%

Investigating the L-Glu-NMDA receptor-H2S-NMDA receptor pathway that regulates gastric function in rats’ nucleus ambiguus

Sun,

Li,

Shi

et al. 2024

Front. Pharmacol.

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BackgroundIn previous investigations, we explored the regulation of gastric function by hydrogen sulfide (H2S) and L-glutamate (L-Glu) injections in the nucleus ambiguus (NA). We also determined that both H2S and L-Glu have roles to play in the physiological activities of the body, and that NA is an important nucleus for receiving visceral sensations. The purpose of this study was to explore the potential pathway link between L-Glu and H2S, resulting in the regulation of gastric function.MethodsPhysiological saline (PS), L-glutamate (L-Glu, 2 nmol), NaHS (2 nmol), D-2-amino-5-phopho-novalerate (D-AP5, 2 nmol) + L-Glu (2 nmol), aminooxyacetic acid (AOAA, 2 nmol) + L-Glu (2 nmol), D-AP5 (2 nmol) + NaHS (2 nmol) were injected into the NA. A balloon was inserted into the stomach to observe gastric pressure and for recording the changes of gastric smooth muscle contraction curve. The gastric fluid was collected by esophageal perfusion and for recording the change of gastric pH value.ResultsInjecting L-Glu in NA was found to significantly inhibit gastric motility and promote gastric acid secretion in rats (p < 0.01). On the other hand, injecting the PS, pre-injection N-methyl-D-aspartate (NMDA) receptor blocker D-AP5, cystathionine beta-synthase (CBS) inhibitor AOAA and re-injection L-Glu did not result in significant changes (p > 0.05). The same injection NaHS significantly inhibit gastric motility and promote gastric acid secretion in rats (p < 0.01), but is eliminated by injection D-AP5 (p > 0.05).ConclusionThe results indicate that both exogenous L-Glu and H2S injected in NA regulate gastric motility and gastric acid secretion through NMDA receptors. This suggests that NA has an L-Glu-NMDA receptor-CBS-H2S pathway that regulates gastric function.

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Peripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies

Cited by 14 publications

References 46 publications

Protective Effects of Borago officinalis (Borago) on Cold Restraint Stress-Induced Gastric Ulcers in Rats: A Pilot Study

Protective Effects of Borago officinalis (Borago) on Cold Restraint Stress-Induced Gastric Ulcers in Rats: A Pilot Study

Red and near‐infrared light induces intracellular Ca²⁺ flux via the activation of glutamate N‐methyl‐D‐aspartate receptors

Investigating the L-Glu-NMDA receptor-H2S-NMDA receptor pathway that regulates gastric function in rats’ nucleus ambiguus

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Peripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies

Cited by 14 publications

References 46 publications

Protective Effects of Borago officinalis (Borago) on Cold Restraint Stress-Induced Gastric Ulcers in Rats: A Pilot Study

Protective Effects of Borago officinalis (Borago) on Cold Restraint Stress-Induced Gastric Ulcers in Rats: A Pilot Study

Red and near‐infrared light induces intracellular Ca2+ flux via the activation of glutamate N‐methyl‐D‐aspartate receptors

Investigating the L-Glu-NMDA receptor-H2S-NMDA receptor pathway that regulates gastric function in rats’ nucleus ambiguus

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Red and near‐infrared light induces intracellular Ca²⁺ flux via the activation of glutamate N‐methyl‐D‐aspartate receptors