Peripheral nerve injury results in a biased loss of sensory neuron sub-populations

Abstract

There is a rich literature describing loss of dorsal root ganglion (DRG) neurons following peripheral axotomy, but the vulnerability of discrete subpopulations has not yet been characterised. Furthermore, the extent and even presence of neuron loss following injury has recently been challenged. In this study, we have used a range of transgenic recombinase driver mouse lines to genetically label molecularly defined subpopulations of DRG neurons and track their survival following traumatic nerve injury. We find that spared nerve injury (SNI) leads to a marked loss of cell containing DRG-volume and a concomitant loss of small diameter DRG neurons. Neuron loss occurs unequally across subpopulations and is particularly prevalent in non-peptidergic nociceptors, marked by expression of Mrgprd. We show that this subpopulation is almost entirely lost following SNI and severely depleted (by roughly 50%) following sciatic nerve crush. Finally, we used anin vitromodel of DRG neuron survival to demonstrate that non-peptidergic nociceptor loss is likely dependent on the absence of neurotrophic support. Together, these results profile the extent to which DRG neuron subpopulations can survive axotomy, with implications for our understanding of nerve injury-induced plasticity and pain.

Satellite glial cells from adult DRG dedifferentiatein vitroand can be reprogrammed into nociceptor-like neurons

“…About half of these patients exhibit complete loss of neurons and SGCs in the injury-affected DRG (Sodmann et al, 2024). Other studies indicate a loss of specific nociceptor subtypes following injury (Cooper et al, 2023). Therefore, replacement of lost or dysfunctional neurons or adding supporting cells may be a strategy to treat sensory loss and neuropathic pain.…”