Peripheral Nerve Regeneration: Current Status and New Strategies Using Polymeric Materials

Pinho, Ana C.; Fonseca, A. C.; Serra, Arménio C.; Santos, J. D.; Coelho, Jorge F. J.

doi:10.1002/adhm.201600236

Cited by 87 publications

(90 citation statements)

References 131 publications

(167 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5,8,9 As an alternative, most of the current research efforts are directed toward the development of nerve guidance conduits (NGCs) from natural or synthetic polymers. 10–12 An NGC is supposed to maintain the nerve regeneration environment, guide axon regeneration, keep the nerve growth factors (NGFs) in the wound healing space, and prevent the invasion of cells that could form scars. 12–14 Among the reported systems, NGCs based on electrospun fibers have received the most attention owing to their highly porous walls and their ability to present the fibers as a uniaxially aligned array.…”

Section: Introductionmentioning

confidence: 99%

“…10–12 An NGC is supposed to maintain the nerve regeneration environment, guide axon regeneration, keep the nerve growth factors (NGFs) in the wound healing space, and prevent the invasion of cells that could form scars. 12–14 Among the reported systems, NGCs based on electrospun fibers have received the most attention owing to their highly porous walls and their ability to present the fibers as a uniaxially aligned array. 15 The aligned fibers can provide a topographical cue to direct and enhance axonal extension during regeneration.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differentiation of Bone Marrow Stem Cells into Schwann Cells for the Promotion of Neurite Outgrowth on Electrospun Fibers

Xue

Yang

O’Connor

et al. 2017

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Seeding nerve guidance conduits with Schwann cells can improve the outcome of peripheral nerve injury repair. Bone marrow stem cells (BMSCs) represent a good choice of cell source as they can differentiate into Schwann cells under appropriate conditions. In this work, we systematically investigated the differentiation of BMSCs into Schwann cells on scaffolds comprising electrospun fibers. We changed the alignment, diameter, and surface properties of the fibers to optimize the differentiation efficiency. The uniaxial alignment of fibers not only promoted the differentiation of BMSCs into Schwann cells but also dictated the morphology and alignment of the derived cells. Coating the surface of aligned fibers with laminin further enhanced the differentiation and thus increased the secretion of neurotrophins. When co-cultured with PC12 cells or chick dorsal root ganglion, the as-derived Schwann cells were able to promote the outgrowth of neurites from cell bodies and direct their extension along the fibers, demonstrating the positive impacts of both the neurotrophic effect and the morphological contact guidance. This work offers a promising strategy for integrating fiber guidance with stem cell therapy to augment peripheral nerve injury repair.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differentiation of Bone Marrow Stem Cells into Schwann Cells for the Promotion of Neurite Outgrowth on Electrospun Fibers

Xue

Yang

O’Connor

et al. 2017

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…In addition, many natural and synthetic polymers with interesting properties can be incorporated into SF as reinforcement . On the benefit of these advantages, SF‐based biomaterials have become increasing popular in the field of peripheral nerve regeneration . Our previous study showed that SF/P(LLA‐CL) nerve conduits promote nerve regeneration more effectively than P(LLA‐CL) conduits .…”

Section: Discussionmentioning

confidence: 99%

“…Currently, nerve conduits made of synthetic or natural polymers have become a promising alternative to autologous nerve grafts. A number of nerve conduits have shown to successfully bridge peripheral nerve gaps and support nerve regeneration …”

Section: Introductionmentioning

confidence: 99%

Silk fibroin enhances peripheral nerve regeneration by improving vascularization within nerve conduits

Wang

Jia

Yang

et al. 2018

J Biomedical Materials Res

View full text Add to dashboard Cite

Silk fibroin (SF)-based nerve conduits have been widely used to bridge peripheral nerve defects. Our previous study showed that nerve regeneration in a SF-blended poly (l-lactide-co-ɛ-caprolactone) [P(LLA-CL)] nerve conduit is better than that in a P(LLA-CL) conduit. However, the involved mechanisms remain unclarified. Because angiogenesis within a nerve conduit plays an important role in nerve regeneration, vascularization of SF/P(LLA-CL) and P(LLA-CL) conduits was compared both in vitro and in vivo. In the present study, we observed that SF/P(LLA-CL) nanofibers significantly promoted fibroblast proliferation, and vascular endothelial growth factor secreted by fibroblasts seeded in SF/P(LLA-CL) nanofibers was more than seven-fold higher than that in P(LLA-CL) nanofibers. Conditioned medium of fibroblasts in the SF/P(LLA-CL) group stimulated more human umbilical vein endothelial cells (HUVEC) to form capillary-like networks and promoted faster HUVEC migration. The two kinds of nerve conduits were used to bridge 10-mm-length nerve defects in rats. At 3 weeks of reparation, the blood vessel area in the SF/P(LLA-CL) group was significantly larger than that in the P(LLA-CL) group. More regenerated axons and Schwann cells were also observed in the SF/P(LLA-CL) group, which was consistent with the results of blood vessels. Collectively, our data revealed that the SF/P(LLA-CL) nerve conduit enhances peripheral nerve regeneration by improving angiogenesis within the conduit. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2070-2077, 2018.

show abstract

“…Peripheral nerve injury is a common disease across the world, which can be caused by traffic accidents, work accidents, natural disasters, warfare, tumor damage, etc . Nerve scaffolds are often used to bridge large nerve defects.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Neurite Outgrowth on a Multiblock Conductive Nerve Scaffold with Self‐Powered Electrical Stimulation

Sun

Meng

et al. 2019

Adv Healthcare Materials

View full text Add to dashboard Cite

Electrical stimulation (ES) is widely applied to promote nerve regeneration. Currently, metal needles are used to exert external ES, which may cause pain and risk of infection. In this work, a multiblock conductive nerve scaffold with self‐powered ES by the consumption of glucose and oxygen is prepared. The conductive substrate is prepared by in situ polymerization of polypyrrole (PPy) on the nanofibers of bacterial cellulose (BC). Platinum nanoparticles are electrodeposited on the anode side for glucose oxidation, while nitrogen‐doped carbon nanotubes (N‐CNTs) are loaded on the cathode side for oxygen reduction. The scaffold shows good mechanical property, flexibility and conductivity. The scaffold can form a potential difference of above 300 mV between the anode and the cathode in PBS with 5 × 10−3 m glucose. Dorsal root ganglions cultured on the Pt‐BC/PPy‐N‐CNTs scaffold are 55% longer in mean neurite length than those cultured on BC/PPy. In addition, in vivo study indicates that the Pt‐BC/PPy‐N‐CNTs scaffold promotes nerve regeneration compared with the BC/PPy group. This paper presents a novel design of a nerve scaffold with self‐powered ES. In the future, it can be combined with other features to promote nerve regeneration.

show abstract

Peripheral Nerve Regeneration: Current Status and New Strategies Using Polymeric Materials

Cited by 87 publications

References 131 publications

Differentiation of Bone Marrow Stem Cells into Schwann Cells for the Promotion of Neurite Outgrowth on Electrospun Fibers

Differentiation of Bone Marrow Stem Cells into Schwann Cells for the Promotion of Neurite Outgrowth on Electrospun Fibers

Silk fibroin enhances peripheral nerve regeneration by improving vascularization within nerve conduits

Enhanced Neurite Outgrowth on a Multiblock Conductive Nerve Scaffold with Self‐Powered Electrical Stimulation

Contact Info

Product

Resources

About