Peripheral selection of V delta 1+ cells with restricted T cell receptor delta gene junctional repertoire in the peripheral blood of healthy donors.

Beldjord, Kheïra; Beldjord, Chérif; Macintyre, Elizabeth; Even, P; Sigaux, François

doi:10.1084/jem.178.1.121

Cited by 43 publications

(34 citation statements)

References 33 publications

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“…Our observations support the model that the peripheral V␦1 T cell repertoire is shaped by positive selection in response to an antigenic stimulation (47,48).…”

Section: Discussionsupporting

confidence: 88%

γδ+ T Cells Involvement in Viral Immune Control of Chronic Human Herpesvirus 8 Infection

Barcy¹,

Rosa²,

Vieira³

et al. 2008

The Journal of Immunology

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Little is known about what effector populations are associated with the control of human herpesvirus 8 (HHV-8) infection in vivo. We compared T lymphocyte subsets among HIV−HHV-8+ and HIV−HHV-8− infected human individuals. αβ+ T cells from HHV-8-infected individuals displayed a significantly higher percentage of differentiated effector cells among both CD4+ and CD8+ T cell subsets. HHV-8 infection was associated with significant expansion of γδ+ Vδ1 T cells expressing a differentiated effector cell phenotype in peripheral blood. In vitro stimulation of PBMC from HHV-8-infected individuals with either infectious viral particles or different HHV-8 viral proteins resulted in γδ Vδ1 T cell activation. In addition, γδ Vδ1 T cells displayed a strong reactivity against HHV-8-infected cell lines and prevented the release of infectious viral particles following the induction of lyric replication. These data indicate that γδ T cells play a role in both innate and adaptive T cell responses against HHV-8 in immunocompetent individuals.

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“…Our observations support the model that the peripheral V␦1 T cell repertoire is shaped by positive selection in response to an antigenic stimulation (47,48).…”

Section: Discussionsupporting

confidence: 88%

γδ+ T Cells Involvement in Viral Immune Control of Chronic Human Herpesvirus 8 Infection

Barcy¹,

Rosa²,

Vieira³

et al. 2008

The Journal of Immunology

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“…[13][14][15] No physiologic or environmental cause was proposed to explain this phenomenon. We showed in this study that the restriction of the V␦1 T-cell repertoire is associated with CMV but not with HSV, VZV, or EBV infection.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have reported that restricted repertoire of V␦1 T cells can be found in some healthy persons, but the reason for this is unknown. [13][14][15]28 To address this issue, complementary determining region 3 (CDR3) size distributions of TCR V␦1 chains expressed by PBMCs were analyzed by the Immunoscope technique in 10 CMV ؉ and 10 CMV Ϫ healthy blood donors. As shown in Figure 2, the repertoire of V␦1 T cells, but not that of V␦2 T cells, was more restricted in seropositive patients than in seronegative patients.…”

Section: Influence Of CMV Infection On the Repertoire Of V␦1 ␥␦ T Cellsmentioning

confidence: 99%

“…Their repertoire in the peripheral blood of most healthy adults is restricted, whereas it is polyclonal in the thymus and in cord blood. [13][14][15] This observation suggests that unknown environmental factors encountered during adult life are selectively amplifying the V␦1 T lymphocytes subpopulation. The ligand specificity of V␦1 ␥␦ T cells has been much less explored than that of V␥9V␦2 T cells, even though some in vitro isolated V␦1 ␥␦ T-cell lines or clones have been reported to be activated by CD1c 16 or by MHC class I-related chain A or B.…”

Section: Introductionmentioning

confidence: 99%

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Long-term expansion of effector/memory Vδ2− γδ T cells is a specific blood signature of CMV infection

Pitard

Roumanes

Lafarge

et al. 2008

Blood

193

226

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The ability of human ␥␦ T cells to develop immunologic memory is still a matter of debate. We previously demonstrated the involvement of V␦2 ؊ ␥␦ T lymphocytes in the response of immunosuppressed organ recipients to cytomegalovirus (CMV). Here, we demonstrate their ability to mount an adaptive immune response to CMV in immunocompetent subjects. V␦2 ؊ ␥␦ T-cell peripheral blood numbers, repertoire restriction, and cytotoxicity against CMV-infected fibroblasts were markedly increased in CMV-seropositive, compared with CMV-seronegative, healthy persons. Whereas V␦2 ؊ ␥␦ T cells were found as naive cells in CMV ؊ patients, they virtually all exhibited the cytotoxic effector/ memory phenotype in CMV ؉ patients, which is also observed in transplanted patients challenged with CMV. This longterm complete remodeling of the V␦2 ؊ ␥␦ T-cell population by CMV predicts their ability to exhibit an adaptive anti-CMV immune response. Consistent with this, we observed that the secondary response to CMV was associated with a faster ␥␦ T-cell expansion and a better resolution of infection than the primary response. In conclusion, the increased level of effectormemory V␦2 ؊ IntroductionHuman cytomegalovirus (CMV) is a widespread ␤-herpesvirus that establishes a lifelong viral persistence without detectable symptoms in immunocompetent patients but with life-threatening consequences in immunologically immature or compromised patients. Many studies have been reported that support an important role for adaptive T lymphocytes in the control of CMV infection. [1][2][3][4] In addition, we have demonstrated that the ␥␦ T-cell subpopulation contributes to the anti-CMV immune responses. 5,6 These unconventional T cells are generally considered to be intermediates between innate and adaptive immunity because of their rapid and massive responses to very diverse immune challenges.Compelling data exist that demonstrate the importance of ␥␦ T cells in various microbial infections in humans. They exhibit in vitro reactivity against cells infected by viruses, bacteria, or parasites and are selectively expanded in the peripheral blood of infected patients. [7][8][9][10] The majority of human circulating ␥␦ T cells express a T-cell receptor (TCR) encoded by the V␥9 and V␦2 gene segments. These cells are activated after interaction with nonpeptidic phosphorylated compounds, collectively called phosphoantigens, which are metabolic intermediates of the isoprenoid biosynthetic pathway. 11,12 These phosphoantigens are expressed by a variety of bacteria and parasites and are also present in some tumors. The other ␥␦ T cells are known as V␦2 Ϫ ␥␦ T cells, are largely located in mucosal epithelia and in the spleen, and represent approximately 20% of all circulating ␥␦ T cells. These cells predominantly express a TCR containing the V␦1 region. Their repertoire in the peripheral blood of most healthy adults is restricted, whereas it is polyclonal in the thymus and in cord blood. [13][14][15] This observation suggests that unknown environmental factors encou...

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“…As shown in Figure 8, Vδ1 sequences recovered before CMV infection were highly diverse, but a few of them were found 2-4 times. These repeated sequences, which are not uncommon in healthy adults (41), probably reflect previous clonal expansions driven by recurrent peripheral antigens. Consistent with Immunoscope studies, CMV infection resulted in profound changes of the Vδ1 repertoire.…”

Section: Recurrent Junctional Motifs In Vδ1 + T Cells Of CMV + Patientsmentioning

confidence: 99%

Implication of γδ T cells in the human immune response to cytomegalovirus

Déchanet¹,

Merville²,

Lim³

et al. 1999

J. Clin. Invest.

296

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Peripheral selection of V delta 1+ cells with restricted T cell receptor delta gene junctional repertoire in the peripheral blood of healthy donors.

Cited by 43 publications

References 33 publications

γδ+ T Cells Involvement in Viral Immune Control of Chronic Human Herpesvirus 8 Infection

γδ+ T Cells Involvement in Viral Immune Control of Chronic Human Herpesvirus 8 Infection

Long-term expansion of effector/memory Vδ2− γδ T cells is a specific blood signature of CMV infection

Implication of γδ T cells in the human immune response to cytomegalovirus

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