2020
DOI: 10.1101/2020.09.18.302950
|View full text |Cite
Preprint
|
Sign up to set email alerts
|

Periplasmic Expression of SpyTagged Antibody Fragments Enables Rapid Modular Antibody Assembly

Abstract: Antibodies are essential tools in research and diagnostics. While antibody fragments can be rapidly produced in Escherichia coli, full-length antibodies with an Fc region or antibodies modified with probes are time and labor intensive in production.SpyTag/SpyCatcher protein ligation technology could covalently attach such functionalities to antibody fragments equipped with a SpyTag. However, we found that the necessarily periplasmic expression of such antibody fragments in E. coli led to rapid cleavage of the … Show more

Help me understand this report
View published versions

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

0
0
0

Publication Types

Select...

Relationship

0
0

Authors

Journals

citations
Cited by 0 publications
references
References 49 publications
(53 reference statements)
0
0
0
Order By: Relevance

No citations

Set email alert for when this publication receives citations?