Peritoneal cavity phagocytes from the teleost sea bass express a glucocorticoid receptor (cloned and sequenced) involved in genomic modulation of the in vitro chemiluminescence response to zymosan

Vizzini, Aiti; Vazzana, Mirella; Cammarata, Matteo; Parrinello, Nicolò

doi:10.1016/j.ygcen.2006.07.016

Cited by 51 publications

(47 citation statements)

References 53 publications

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“…Vizzini et al (2007) showed that the increased cortisol level (1 h) in vitro decreased sea bass leucocyte CL via the binding to the glucocorticoid receptor DlGR1 (Vizzini et al, 2007). In mice, the expression of the glucocorticoid receptor mRNA was down-regulated in the brain and spleen due to the social hierarchy distruption (SDR) Quan et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…In fish, the primary response to stress involves the activation of the hypothalamic-pituitary-interrenal axis, which leads to increased levels of adrenocorticotropic hormone, cortisol, catecholamine and several plasmatic parameters and physiological activities, including the immune response (Fevolden et al, 2002;Vizzini et al, 2007;Vazzana et al, 2008Vazzana et al, , 2010. In aquaculture stress induced by confinement and by a high-density population increases blood plasma levels of cortisol, glucose, and osmolarity and affect innate immune responses.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, in vivo and in vitro experiments showed that increased cortisol levels affect phagocytosis via the cytosolic receptor DlGR1 in sea bass (Vizzini et al, 2007). This receptor was localised to the head kidney, spleen, gills, intestine, heart and liver tissues (Vazzana et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Seabream (Sparus aurata) long-term dominant-subordinate interplay affects phagocytosis by peritoneal cavity cells

Cammarata

Vazzana

Accardi

et al. 2012

Brain, Behavior, and Immunity

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a b s t r a c tFish are sensitive to stressful conditions that affect their innate immune systems and increase their susceptibility to diseases. We examined the social stress of paired gilthead seabream (Sparus aurata). Social hierarchies (dominant/subordinate) were characterised by behavioural changes, such as ''aggressiveness'' and ''feeding order''; hierarchical positions were established within an hour of exposure to social stress and remained unchanged for approximately 1 year.To characterise physiological stress, we measured blood plasma levels of cortisol, glucose, and lactate as well as osmolarity and observed that the levels of these stress markers were higher in subordinate individuals than in dominant ones. The discriminant analysis revealed a separation of the subordinate fish groups, and at 15 days, a significant separation among groups was observed.Moreover, diminished phagocytic and respiratory burst activities revealed that social stress appeared to affect the cellular innate immune response of the subordinate specimens.Finally, to examine the effect of cortisol on phagocytosis, peritoneal cavity cells were treated in vitro, and an inhibitory effect was observed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Seabream (Sparus aurata) long-term dominant-subordinate interplay affects phagocytosis by peritoneal cavity cells

Cammarata

Vazzana

Accardi

et al. 2012

Brain, Behavior, and Immunity

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“…The oocyte concentrations of the steroid RU486 could not be measured, but it was assumed that the partitioning of RU486 between the ovarian fluid and the ooplasm was similar to that for cortisol. The concentration of RU486 in the ovarian fluid was in a range that has been shown to have antagonistic effects on GR function in several species of fish (Vijayan et al 1994, Aluru et al 2004, Scott et al 2005, Veillette et al 2007, Vizzini et al 2007, Alderman et al 2012.…”

Section: K1mentioning

confidence: 98%

“…This study provided evidence that suggests a key role for GR in the modulation of growth, possibly via changes in the expression of several IGF system-related genes, including both the genes that encode for the peptide hormones, IGF1 and 2, and their protein receptors. An important component of the study was the use of the progesterone receptor (PR) and GR antagonist, RU486 (mifepristone), which has been used previously for the evaluation of GR function in fish and mammals (Vijayan et al 1994, Pariante et al 2001, Aluru et al 2004, Scott et al 2005, Veillette et al 2007, Vizzini et al 2007, Peeters et al 2008, Du et al 2009, Alderman et al 2012. Table 3 The effects of in ovo cortisol and RU486 treatment on the correlation between the expressions of igf system genes and gr genes in 7-days post-fertilization (dpf) embryos The antagonistic properties of RU486 on PRs and GRs are complex and still not fully understood; the PR antagonistic action of RU486 has received most attention (reviewed by Leonhardt & Edwards (2002)).…”

Section: K1mentioning

confidence: 99%

Glucocorticoid receptor activation following elevated oocyte cortisol content is associated with zygote activation, early embryo cell division, and IGF system gene responses in rainbow trout

Li¹,

Leatherland²,

Vijayan³

et al. 2012

Journal of Endocrinology

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Increased in ovo cortisol content of rainbow trout oocytes from w3 . 5 to w5 . 0 ng.oocyte K1 before fertilization enhances the growth of embryos and juveniles and changes the long-term expression pattern of IGF-related genes. This study used embryos reared from oocytes enriched with cortisol and the glucocorticoid receptor (GR) antagonist, RU486, to determine whether the growth-promoting actions of cortisol involve GR protein activation and modulation of gr expression. Whole-mount in situ immunohistofluorescence studies of zygotes showed that enhanced oocyte cortisol increased the immunofluorescent GR signal and activated the relocation of GR from a general distribution throughout the cytoplasm to an accumulation in the perinuclear cytoplasm. In ovo cortisol treatment increased the number of embryonic cells within 48-h post-fertilization, and RU486 partially suppressed this cortisol stimulation of cell duplication. In addition, there was complex interplay between the expression of gr and igf system-related genes spatiotemporally in the different treatment groups, suggesting a role for GR in the regulation of the expression of development. Taken together, these findings indicate an essential role for GR in the regulation of epigenomic events in very early embryos that promoted the long-term growth effects of the embryos and juvenile fish. Moreover, the pretreatment of the oocyte with RU486 had a significant suppressive effect on the maternal mRNA transcript number of gr and igf system-related genes in oocytes and very early stage embryos, suggesting an action of antagonist on the stability of the maternal transcriptome.

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Characterization of Oryzias latipes glucocorticoid receptors and their unique response to progestins

Miyagawa

Lange

Tohyama

et al. 2014

J of Applied Toxicology

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Various receptor bioassays, including estrogens, androgens and thyroid hormones, have been developed and applied successfully for assessing hormone function in a wide range of animal species, including fish. In fish, corticosteroids play a pivotal role in physiology as they do in mammals, but far less is known about the corticosteroid receptor system in fish compared with in mammals. Here we established a transient transactivation assay using the Japanese medaka, Oryzias latipes, glucocorticoid receptors (olGRs) and mineralocorticoid receptor to analyse their functional properties in a fish. We found that olGR2 was highly responsive to glucocorticoids, similar to the human GR, whereas the olGR1 subtype was minimally responsive. Thus, olGR2 most likely mediates glucocorticoid signaling in medaka. We further tested crosstalk between GRs and other steroid hormones, and found that progestins could activate or inactivate olGR2-mediating transcription, depending on the presence or absence of cortisol. The transactivation assays developed for medaka GRs provide tools to gain useful insights into corticosteroid signaling in fish and for in vitro screening of environmental substances activating GRs.

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Peritoneal cavity phagocytes from the teleost sea bass express a glucocorticoid receptor (cloned and sequenced) involved in genomic modulation of the in vitro chemiluminescence response to zymosan

Cited by 51 publications

References 53 publications

Seabream (Sparus aurata) long-term dominant-subordinate interplay affects phagocytosis by peritoneal cavity cells

Seabream (Sparus aurata) long-term dominant-subordinate interplay affects phagocytosis by peritoneal cavity cells

Glucocorticoid receptor activation following elevated oocyte cortisol content is associated with zygote activation, early embryo cell division, and IGF system gene responses in rainbow trout

Characterization of Oryzias latipes glucocorticoid receptors and their unique response to progestins

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