Peritoneal Fluid Cytokines and the Differential Diagnosis of Benign and Malignant Ovarian Tumors and Residual/Recurrent Disease Examination

Abstract

This study aimed to assess the potential value of peritoneal fluid cytokine examination for the differential diagnosis of ovarian tumors and for evaluating residual or recurrent disease after treatment. The cytokines that are commonly elevated in ovarian cancer, VEGF, IL-6, bFGF, IL-8 and M-CSF, and a reference ovarian tumor marker, CA 125, were measured in peritoneal fluids of 53 previously untreated patients with epithelial ovarian cancer, 18 ovarian cancer patients after surgical treatment and chemotherapy, and 17 patients with benign epithelial ovarian tumors. Non-parametric statistical analysis of data was performed. Ovarian cancer peritoneal fluids, as compared to peritoneal fluids of patients with benign ovarian tumors, contained significantly higher concentrations of IL-6, VEGF and CA 125, and significantly lower concentrations of bFGF and M-CSF, but only the levels of IL-6 and VEGF were significantly higher in peritoneal fluids of stage I and II ovarian cancer patients than of patients with benign ovarian conditions. IL-6 at the cutoff level of 400 pg/mL discriminated benign and malignant ovarian tumors with 92% sensitivity and 60% specificity, while VEGF at the cutoff of 400 pg/mL had 90% sensitivity and 80% specificity. At the cutoff level of 1200 pg/mL, IL-6 had 84% sensitivity and 87% specificity. A radical decrease in local cytokine and CA 125 levels in patients after treatment was independent of therapy outcome. IL-6 and VEGF measurements in peritoneal fluids might be useful for the differential diagnosis of malignant and benign ovarian conditions, but not for residual or recurrent disease examination. (Int J Biol Markers 2007; 22: 172-80)

Cell-free ascites from ovarian cancer patients induces Warburg metabolism and cell proliferation through TGFβ-ERK signaling

“…Ascites, formed in the peritoneal cavity, is an important constituent of the ovarian cancer tumor microenvironment. Ascites has a very complex composition that includes cytokines and inflammatory regulators [8][9][10][11], adipokines [12][13][14], extracellular vesicles [15][16][17][18], transforming growth factor beta (TGFβ)-induced [19], vascular endothelial growth factor [13], growth factor receptor ligands [20], factors regulating cellular adhesion [8], metabolites [8], cholesterol [21,22], bacteria, and bacterial metabolites (reviewed in [23]). Ascites supports transcoelomic metastasis formation (dissemination) of the primary tumor in the peritoneum.…”

Aparato de importação de proteínas mitocondriais em Aspergillus fumigatus: caracterização fenotípica da deleção da menor subunidade do complexo TIM23

“…al., 2003). Já o M-CSF (fator estimulador de colônias de macrófagos), ao ser utilizado sozinho foi capaz de detectar o OvCa com uma especificidade de cerca de 61 -68% e especificidade de 93%, mas os melhores resultados para a detecção foram obtidos apenas quando utilizado em conjunto com CA-125(Chechlinska et. al., 2007).1.3 Proteômica e o câncer de ovárioA proteômica emergiu como uma tecnologia poderosa para decifrar e integrar os processos biológicos a partir do estudo de todas as proteínas de um sistema biológico.…”