Peritoneal fluid, endometriosis, and ciliary beat frequency in the human fallopian tube

Lyons, R.A.; Djahanbakhch, O.; Sarıdoğan, Ertan; Naftalin, A. A.; Mahmood, Tariq; Weekes, A. R. L.; Chenoy, Rashna

doi:10.1016/s0140-6736(02)11247-5

Cited by 71 publications

(27 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although a systemic review [18] found no significant differences in long term follow-up concerning repeated ectopic and intrauterine pregnancies in natural cycles with respect to the treatment assessed (medical, surgical or combined, respectively), we hypothesize, that a TEP as well as the conservative and the surgical treatment causes alterations in tubal motility, ciliarfunction, and uterus contractions [19][20][21].…”

Section: Resultsmentioning

confidence: 80%

Previous tubal ectopic pregnancy raises the incidence of repeated ectopic pregnancies in In Vitro fertilization-embryo transfer patients

Weigert

Gruber

Pernicka

et al. 2008

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To investigate the incidence of Tubal Ectopic Pregnancies (TEP) in IVF-ET patients with respect to the status of the fallopian tubes after a previous TEP. Material and methods This retrospective study compares patients undergoing 481 IVF-ET cycles after conservatively or surgically treated TEP(s) with a Control Group (idiopathic or male factor for IVF-ET indication). Medical reports of surgery and/or hysterosalpingograms prior to the IVF cycles classified the status of the fallopian tubes. Results 12 TEPs (8.95%/Pregnancies (PR)) occurred in the Study Group. In the Control Group one TEP (0.75%/PR; p<0.001) was found. Smoking increased the probability of TEPs (p=0.0028) and of pathological pregnancies (abortion, biochemical and ectopic PR; (p=0.0411)). For statistic evolution logistic regression (PROC GENMOD) and a repeated measure model were applied. Conclusion Women with a previous TEP should be informed about the significantly increased risk for a further TEP in IVF-ET treatment, especially if they are smoking.

show abstract

Section: Resultsmentioning

confidence: 80%

Previous tubal ectopic pregnancy raises the incidence of repeated ectopic pregnancies in In Vitro fertilization-embryo transfer patients

Weigert

Gruber

Pernicka

et al. 2008

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…For example, cilia beating frequency is an essential function of the fallopian tube to transport sperm, the COC, and the embryo to the uterus. The role of the cilia is critical in fertility as demonstrated by rodent studies where muscular activity of the tube was abolished without any change in tubal transport time of the ovum, and immotile cilia in women is associated with reduced fertility (McComb et al, 1986;Halbert et al, 1989;Lyons et al, 2002b). In the current study, using digital microscopy, the rate of cilia beating was monitored in response to purified hormones mimicking the menstrual cycle.…”

Section: Discussionmentioning

confidence: 99%

Human fallopian tube epithelium co-culture with murine ovarian follicles reveals crosstalk in the reproductive cycle

Zhu

Rashedi

et al. 2016

Mol. Hum. Reprod.

View full text Add to dashboard Cite

STUDY QUESTION: Do interactions between human fallopian tube epithelium and murine follicles occur during an artificial reproductive cycle in a co-culture system in vitro?SUMMARY ANSWER: In a co-culture system, human fallopian tissues responded to the menstrual cycle mimetic by changes in morphology and levels of secreted factors, and increasing murine corpus luteum progesterone secretion.WHAT IS KNOWN ALREADY: The entire fallopian tube epithelium, including ciliated and secretory cells, can be regulated in the reproductive cycle. Currently, there are no in vitro culture models that can monitor fallopian tissues in real time in response to factors produced by the ovary. In addition, there are no reports on the impact of fallopian tissue on ovarian function during the menstrual cycle.STUDY DESIGN, SAMPLES/MATERIALS, METHODS: Human fallopian tissue (n = 24) was obtained by routine hysterectomies from women (aged 26-50 years, mean age = 43.6) who had not undergone exogenous hormonal treatment for at least 3 months prior to surgery. CD1 female mice were used for ovarian follicle isolation. The human fallopian epithelium layers were either co-cultured with five murine multilayer secondary follicles (150-180 μm follicles, encapsulated in one alginate gel bead) for 15 days or received stepwise steroid hormone additions for 13 days. The fallopian tissue morphology and cilia beating rate, as measured by an Andor Spinning Disk Confocal, were investigated. Oviduct-specific glycoprotein 1 (OVGP1), human insulin-like growth factor 1 (hIGF1), vascular endothelial growth factor A (VEGF-A) and interleukin 8 (IL8) as biological functional markers were measured either by ELISA or western blot to indicate dynamic changes in the fallopian epithelium during the reproductive cycle generated by mouse follicles or by stepwise steroid hormone induction. Three or four patients in each experiment were recruited for replicates. Data were presented as mean ± SD and further analyzed using one-way ANOVA followed by Tukey's multiple comparisons test. MAIN RESULTS AND THE ROLE OF CHANCE:The cultured fallopian tube epithelium responded to exogenous steroid hormone stimulation, as demonstrated by enhanced cilia beating rate (~25% increase, P = 0.04) and an increase in OVGP1 secretion (P = 0.02) in response to 1 nM estradiol (E 2 ) treatment when compared with 0.1 nM E 2 . Conversely, 10 nM progesterone plus 1 nM E 2 suppressed cilia beating rate by~30% (P = 0.008), while OVGP1 secretion was suppressed by 0.1 nM E 2 plus 50 nM progesterone (P = 0.002 versus 1 nM E 2 alone). Human fallopian tube epithelium was co-cultured with murine secondary follicles to mimic the human menstrual cycle. OVGP1 and VEGF-A secretion from fallopian tissue was similar with stepwise hormone treatment and when cultured with murine follicles. However, the secretion patterns of hIGF1 and IL8 differed in the luteal phase when comparing steroid treatment with follicle co-culture. In co-culture, hIGF1 secretion was suppressed in the luteal versus follicular phase (P = 0.005)...

show abstract

“…Endometriosis is another pathological pelvic inflammatory process that is known to inhibit ciliary beat frequency (203), and in severe disease leads to significant pelvic scarring and pelvic anatomy distortion, and hence is associated with doubling in the incidence of ectopic pregnancy (152), in addition to the negative impact on endometrial receptivity (discussed later). PCOS has also been associated with a doubling of the rate of ectopic pregnancies, although no mechanism has been proposed (140).…”

Section: Inflammatory Disorders: Endometriosis and Infectionmentioning

confidence: 99%

Physiological Aspects of Female Fertility: Role of the Environment, Modern Lifestyle, and Genetics

Hart

2016

Physiological Reviews

175

119

View full text Add to dashboard Cite

Across the Western World there is an increasing trend to postpone childbearing. Consequently, the negative influence of age on oocyte quality may lead to a difficulty in conceiving for many couples. Furthermore, lifestyle factors may exacerbate a couple's difficulty in conceiving due mainly to the metabolic influence of obesity; however, the negative impacts of low peripheral body fat, excessive exercise, the increasing prevalence of sexually transmitted diseases, and smoking all have significant negative effects on fertility. Other factors that impede conception are the perceived increasing prevalence of the polycystic ovary syndrome, which is further exacerbated by obesity, and the presence of uterine fibroids and endometriosis (a progressive pelvic inflammatory disorder) which are more prevalent in older women. A tendency for an earlier sexual debut and to have more sexual partners has led to an increase in sexually transmitted diseases. In addition, there are several genetic influences that may limit the number of oocytes within the ovary; consequently, by postponing attempts at childbearing, a limitation of oocyte number may become evident, whereas in previous generations with earlier conception this potentially reduced reproductive life span did not manifest in infertility. Environmental influences on reproduction are under increasing scrutiny. Although firm evidence is lacking however, dioxin exposure may be linked to endometriosis, phthalate exposure may influence ovarian reserve, and bisphenol A may interfere with oocyte development and maturation. However, chemotherapy or radiotherapy is recognized to lead to ovarian damage and predispose the woman to ovarian failure.

show abstract

Peritoneal fluid, endometriosis, and ciliary beat frequency in the human fallopian tube

Cited by 71 publications

References 4 publications

Previous tubal ectopic pregnancy raises the incidence of repeated ectopic pregnancies in In Vitro fertilization-embryo transfer patients

Previous tubal ectopic pregnancy raises the incidence of repeated ectopic pregnancies in In Vitro fertilization-embryo transfer patients

Human fallopian tube epithelium co-culture with murine ovarian follicles reveals crosstalk in the reproductive cycle

Physiological Aspects of Female Fertility: Role of the Environment, Modern Lifestyle, and Genetics

Contact Info

Product

Resources

About