Peritrophic matrix proteins

Tellam, Ross L.; Wijffels, Gene; Willadsen, Peter

doi:10.1016/s0965-1748(98)00123-4

Cited by 350 publications

(386 citation statements)

References 66 publications

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“…Strongly bound PM proteins were also observed in Trichoplusia ni (Lepidoptera) larvae (Wang & Granados 2000) and Lucilia cuprina (Diptera) larvae (Elvin et al 1996). The majority of the total PM proteins from L. cuprina are not solubilized by strong denaturants such as SDS used in our experiments (Tellam et al 1999). However, in vitro extractions using the same buffers stated above released a significant amount of proteins from PM of D. urucu-treated larvae (Fig.…”

Section: Resultsmentioning

confidence: 54%

Derris (Lonchocarpus) urucu (Leguminosae) Extract Modifies the Peritrophic Matrix Structure of Aedes aegypti (Diptera:Culicidae)

Gusmão

Páscoa

Mathias

et al. 2002

Mem. Inst. Oswaldo Cruz

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Section: Resultsmentioning

confidence: 54%

Derris (Lonchocarpus) urucu (Leguminosae) Extract Modifies the Peritrophic Matrix Structure of Aedes aegypti (Diptera:Culicidae)

Gusmão

Páscoa

Mathias

et al. 2002

Mem. Inst. Oswaldo Cruz

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“…In the insect digestion systems there is a membrane composed of chitin, proteins (such as peritrophins) and proteoglycans, a peritrophic matrix layer that surrounds the food bolus with the function of offering chemical and mechanical protection as well as defense against pathogens (Terra 1996;Lehane 1997;Tellam et al 1999).…”

Section: Resultsmentioning

confidence: 99%

The effect of sub-lethal doses of Azadirachta indica (Meliaceae) oil on the midgut of Spodoptera frugiperda (Lepidoptera, Noctuidae)

Roel¹,

Dourado

Matias

et al. 2010

Rev. Bras. entomol.

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ABSTRACT. The effect of sub-lethal doses of Azadirachta indica (Meliaceae) oil on the midgut of Spodoptera frugiperda (Lepidoptera, Noctuidae). The fall armyworm, Spodoptera frugiperda, is one of the major field pests for maize production. It is mainly controlled by means of synthetic, and more recently by resistant cultivar of maize expressing Bt toxins. The neem tree, Azadirachta indica, is a plant that can potentially control insects with the advantage of being food and environmental safe. The aim of this study was to assess the effect of neem oil on the development and survival of S. frugiperda caterpillars by assessing histological alterations caused on their midgut. Newly hatched caterpillars were submitted to three neem oil concentrations: 0.006; 0.05; 0.4%, which were added to their artificial diet. Ten 3 rd instar caterpillars, taken from each treatment, were submitted to histological analysis. The alimentary canals from the specimens were fixed in Baker for 12 hours, desiccated and diaphanized in alcohol/xylol (1:1) and xylol. After placing the samples in paraffin, they were sliced in 8 µm sections and stained with hematoxylineosin stain. The neem oil added to the diet of S. frugiperda caused total mortality at dose of 0.4% whilst still in the first instars, prolonged the larval and pupal stages, and reduced the pupal weight. Histo-physiological alterations such as degeneration of the epithelial lining of the midgut and in the peritrophic matrix were found at all concentrations of neem oil.KEYWORDS. Fall armyworm; histological analysis; insecticidal plants; neem. RESUMO. Efeito de doses subletais do óleo de Azadirachta indica (Meliaceae) no mesêntero de Spodoptera frugiperda (J. E. Smith) (Lepidoptera, Noctuidae). A lagarta-do-cartucho, Spodoptera frugiperda, é a mais importante praga da cultura do milho. Esta é usualmente, controlada por inseticidas sintéticos e mais atualmente por meio de variedades resistentes de milho com a toxina Bt. O nim Azadirachta indica é planta com potencial no controle de insetos, que possui as vantagens de segurança alimentar e ambiental. Objetivou-se com este estudo avaliar o efeito do óleo do nim no desenvolvimento e sobrevivência de lagartas de S. frugiperda em decorrência das alterações no mesêntero. As lagartas recém nascidas foram submetidas a três concentrações do óleo de nim: 0,006; 0,05; 0,4%, adicionadas à dieta artificial. Dez lagartas do 3º instar, retiradas de cada tratamento, foram submetidas à análise histológica. O canal alimentar dos indivíduos foi fixado em Baker por 12 horas, desidratado, diafanizado em álcool/xilol (1:1) e xilol. Após a inclusão em parafina, as amostras foram seccionadas em 8 µm e coradas pela técnica de hematoxilina-eosina. Observou-se que o óleo de A. indica adicionado à dieta de S. frugiperda causa: mortalidade total na dosagem 0,4% nos primeiros instares, aumento na duração do período larval e pupal, redução no peso de pupas. Alterações histofisiológicas, como degeneração do epitélio do revestimento do mesêntero, da matriz peritrófi...

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“…The cross sections pretreated with amylase do not eliminate the positive staining by PAS reaction in the columnar cell, suggesting exist glycogen associated with positive PAS material. It is known, that the peritrophic membrane is composed principally of chitin, a linear N-acetyl-D-glucosamine polymer (Tellam et al 1999) and glycoproteins (Tellam et al 1999, Terra 2001. Although it is controversial the chitin positive reaction by PAS (Pearse, 1968), chitin is susceptible to coloration by PAS, if it is associated with mucossubstance (Pearse 1968).…”

Section: Discussionmentioning

confidence: 99%

Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae

Pinheiro¹,

Quagio-Grassiotto²,

Gregório³

2008

Neotrop. entomol.

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Diferenças Morfológicas Regionais das Células Epiteliais ao Longo do Intestino Médio de Larvas deDiatraea saccharalis Fabricius (Lepidoptera: Crambidae)RESUMO -A broca da cana, Diatraea saccharalis Fabricius, é uma praga da cana-de-açúcar e outras plantações. O objetivo deste trabalho foi caracterizar variações morfológicas nas células epiteliais (colunares, caliciformes e regenerativas) ao longo do intestino médio de larvas de D. saccharalis. Fragmentos do intestino médio (anterior, mediano e posterior) foram fi xados e processados para microscopia de luz e eletrônica de varredura. Existem diferenças morfológicas citoquímicas e ultraestruturais nas células epiteliais, dependendo da sua localização no intestino médio. A superfície apical de algumas células colunares exibe projeções citoplasmáticas que aumentam em número e volume da região anterior para a posterior do intestino médio. Existe aumento dos grânulos PAS-positivos (Reação de Schiff) no citoplasma apical das células colunares e regenerativas, da região anterior para a posterior. A câmara das células caliciformes, na região anterior do intestino médio, mostra seu ápice estreito, enquanto que na posterior essa porção da câmara é alargada; as evaginações citoplasmáticas da câmara são pequenas e fi nas no ápice, sendo numerosas, longas e mais espessas na porção basal. Os resultados sugerem que o intestino médio da broca da cana apresenta duas regiões morfologicamente distintas, a anterior e posterior; a região mediana é uma região de transição.PALAVRAS-CHAVE: Morfologia, broca da cana, histoquímica, microscopia eletrônica de varredura, microscopia de luz ABSTRACT -The sugarcane borer, Diatraea saccharalis Fabricius, is a pest to sugarcane and many other crops. This work aims to characterize morphological variability in the epithelial cells (columnar, goblet and regenerative) along the midgut of D. saccharalis larvae. Fragments of the midgut (anterior, middle and posterior regions) were fi xed and processed by light and scanning electron microscopy. There are both cytochemical and ultrastructural differences in the morphology of the epithelial cells, depending on their localization along the midgut. The apical surface of columnar cells shows an increase in both number and size of the apical protrusions from the anterior to the posterior midgut regions.There is an increase in the amount of PAS-positive (Periodic Acid-Schiff Reaction) granules detected in the cytoplasm of both the columnar and regenerative cells, from the anterior to the posterior region. The goblet cell apical surface is narrow in the anterior region, and enlarged in the posterior midgut; the chamber's cytoplasm extrusion are small and thin at the apical cavity surface, being thicker, longer and more numerous at the basal portion of the cavity. Our results suggest that the sugarcane borer midgut has two morphologically different regions, the anterior and the posterior; the middle region is a transitional region.

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Peritrophic matrix proteins

Cited by 350 publications

References 66 publications

Derris (Lonchocarpus) urucu (Leguminosae) Extract Modifies the Peritrophic Matrix Structure of Aedes aegypti (Diptera:Culicidae)

Derris (Lonchocarpus) urucu (Leguminosae) Extract Modifies the Peritrophic Matrix Structure of Aedes aegypti (Diptera:Culicidae)

The effect of sub-lethal doses of Azadirachta indica (Meliaceae) oil on the midgut of Spodoptera frugiperda (Lepidoptera, Noctuidae)

Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae

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