Peritrophic Membranes

Peters, Werner

doi:10.1007/978-3-642-84414-0

Cited by 168 publications

(209 citation statements)

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“…This layer is called peritrophic matrix PM. The PM acts as a protective barrier against various chemical, physical and microbial food components (Peters 1992). In this study, larval mortality caused by methanol extract of D. urucu appears to be related to disruption of PM structure and rupture of midgut cells.…”

Section: Resultsmentioning

confidence: 68%

“…aegypti larvae of DDT resistant strain produce nine times more PM than the susceptible strain (Abedi & Brown 1961). Peters (1992) suggests that this enhanced synthesis of PM, induced by the uptake of DDT, is a unusual insect mechanism of resistance. Therefore, the elimination of toxic chemicals enveloped by PM appears to a defensive physiological response of the insect larvae.…”

Section: Resultsmentioning

confidence: 98%

“…The peritrophic matrix (PM) is an acellular sheet that involves the food separating it from the midgut epithelium (Richards & Richards 1977, Peters 1992, Terra 1996. There are two fundamental types of PM defined by their site of synthesis.…”

mentioning

confidence: 99%

“…It may provides protection to Leishmania parasite from the action of the host digestive enzymes (Pimenta et al 1997). It also protects the insects, at least partially, against chemical attack by potentially toxic agents with much smaller molecular dimensions (Peters 1992, Regev et al 1996, Lehane 1997, Barbehenn & Martin 1997. Thus, the permeability of the PM is not a matter of simple ultrafiltration related to pore size (Derksen & Granados 1988).…”

mentioning

confidence: 99%

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Derris (Lonchocarpus) urucu (Leguminosae) Extract Modifies the Peritrophic Matrix Structure of Aedes aegypti (Diptera:Culicidae)

Gusmão

Páscoa

Mathias

et al. 2002

Mem. Inst. Oswaldo Cruz

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Section: Resultsmentioning

confidence: 68%

Section: Resultsmentioning

confidence: 98%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Derris (Lonchocarpus) urucu (Leguminosae) Extract Modifies the Peritrophic Matrix Structure of Aedes aegypti (Diptera:Culicidae)

Gusmão

Páscoa

Mathias

et al. 2002

Mem. Inst. Oswaldo Cruz

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“…The larval PM is primarily composed of chitin, proteoglycans, and proteins (54). Based on our results, we inferred that the N. bombycis spore may bind to and breach the silkworm's PM prior to infecting the epithelial cell.…”

Section: Discussionmentioning

confidence: 65%

Interaction and Assembly of Two Novel Proteins in the Spore Wall of the Microsporidian Species Nosema bombycis and Their Roles in Adherence to and Infection of Host Cells

et al. 2015

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Microsporidia are obligate intracellular parasites with rigid spore walls that protect against various environmental pressures. Despite an extensive description of the spore wall, little is known regarding the mechanism by which it is deposited or the role it plays in cell adhesion and infection. In this study, we report the identification and characterization of two novel spore wall proteins, SWP7 and SWP9, in the microsporidian species Nosema bombycis. SWP7 and SWP9 are mainly localized to the exospore and endospore of mature spores and the cytoplasm of sporonts, respectively. In addition, a portion of SWP9 is targeted to the spore wall of sporoblasts earlier than SWP7 is. Both SWP7 and SWP9 are specifically colocalized to the spore wall in mature spores. Furthermore, immunoprecipitation, far-Western blotting, unreduced SDS-PAGE, and yeast two-hybrid data demonstrated that SWP7 interacted with SWP9. The chitin binding assay showed that, within the total spore protein, SWP9 and SWP7 can bind to the deproteinated chitin spore coats (DCSCs) of N. bombycis. However, binding of the recombinant protein rSWP7-His to the DCSCs is dependent on the combination of rSWP9 -glutathione S-transferase (GST) with the DCSCs. Finally, rSWP9-GST, anti-SWP9, and anti-SWP7 antibodies decreased spore adhesion and infection of the host cell. In conclusion, SWP7 and SWP9 may have important structural capacities and play significant roles in modulating host cell adherence and infection in vitro. A possible major function of SWP9 is as a scaffolding protein that supports other proteins (such as SWP7) that form the integrated spore wall of N. bombycis.M icrosporidia, which infect numerous vertebrate and invertebrate species, are a specific group of obligate spore-forming, intracellular, and unicellular eukaryotic protists (1). The microsporidian species Nosema bombycis causes pebrine, a disease that was common in Europe, America, and Asia during the mid19th century and still causes heavy losses in silk-producing countries, such as China and India (1). It was first recognized in 1857 by Nägeli (2). Recently, phylogenetic analyses based on conserved proteins (3-5), ribosomal DNA (rDNA) sequences (6, 7), and complete sequencing of the Encephalitozoon cuniculi genome (8) have suggested that microsporidia, which undergo a highly reductive evolution, are closely related to fungi (9). However, the N. bombycis genome has greatly expanded due to transposable elements and gene duplications (10).The microsporidian spore wall, which is usually comprised of an electron-dense proteinaceous outer layer (exospore) of 25 to 30 nm and an electron-transparent chitinous inner layer (endospore) of 30 to 35 nm, maintains the spore's morphology and helps the mature spore resist the outer environment (11-13). Although chitin is the major component of the spore wall, only one chitin-associated protein has been identified. The chitin deacetylase activity of E. cuniculi (EcCDA) has been confirmed to be expressed during sporogonic stages (14). Currently, the deprot...

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Legume seed vicilins (7S storage proteins) interfere with the development of the cowpea weevil (Callosobruchus maculatus (F))

Yunes

Andrade

Sales

et al. 1998

J. Sci. Food Agric.

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Vicilins (7S storage proteins) isolated from the seeds of the legumes Vigna unguiculata (cowpea), Vigna angularis (adzuki bean), Canavalia ensiformis (jack bean), Glycine max (soybean), Phaseolus vulgaris (common bean) and Phaseolus lunatus (lima bean) were shown to be immunologically related and to bind to a chitin matrix. The effect of the isolated vicilins on the development of the cowpea weevil Callosobruchus maculatus was examined. Vicilins from all non‐host seeds, including those of the C maculatus‐resistant cowpea line IT81D‐1045, strongly inhibited larval development (ED50 of 1·07±0·32% to 2·22±0·64%). Vicilins from the C maculatus‐susceptible cowpea CE‐31 and adzuki bean seeds were the exception with ED50 of 6·25±0·75% and 5·40±1·54%, respectively. These results correlate well with the host range of C maculatus and are possibly a reflection of the low digestibility of vicilins by insect midgut proteinases in addition to the ability they show in binding to chitin‐containing structures like the ones found in the bruchid midguts. © 1998 SCI.

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Peritrophic Membranes

Cited by 168 publications

References 0 publications

Derris (Lonchocarpus) urucu (Leguminosae) Extract Modifies the Peritrophic Matrix Structure of Aedes aegypti (Diptera:Culicidae)

Derris (Lonchocarpus) urucu (Leguminosae) Extract Modifies the Peritrophic Matrix Structure of Aedes aegypti (Diptera:Culicidae)

Interaction and Assembly of Two Novel Proteins in the Spore Wall of the Microsporidian Species Nosema bombycis and Their Roles in Adherence to and Infection of Host Cells

Legume seed vicilins (7S storage proteins) interfere with the development of the cowpea weevil (Callosobruchus maculatus (F))

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