Perivascular noradrenergic and peptide-containing nerves show different patterns of changes during development and ageing in the guinea-pig

Dhall, Usha; Cowen, T.; Haven, A. J.; Burnstock, Geoffrey

doi:10.1016/0165-1838(86)90003-2

Cited by 93 publications

(27 citation statements)

References 31 publications

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“…The proportion in population density of these types of nerve fibers immunoreactive to these four neuropeptides are consistent with what has been reported for various arterial systems of the guinea pig ; the noradrenergic fibers are more numerous than any of the peptide-containing fibers, and among the nerve fibers immunoreactive to neuropeptides, a decreasing order in population density is seen as follows : CGRP-, SP-, and VIP-immunoreactive fibers (Dhall et al 1986). The population density of each type of immunoreactive fiber increased in more peripheral portions of the artery supplying the distal regions of the dog forepaw.…”

Section: Discussionsupporting

confidence: 75%

Immunohistochemical Study of the Sympathetic and Sensory Innervation to the Blood Vessels of the Dog Forepaw.

Tsuruta

Masuko

Watanabe

1992

Tohoku J. Exp. Med.

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Immunohistochemical staining of arteries supplying the dog forepaw showed a dense distribution of nerve fibers which were immunoreactive to tyrosine hydroxylase (TH), neuropeptide Y (NPY), vasoactive intestinal peptide (VIP), substance P (SP), and calcitonin gene-related peptide (CGRP) around the vascular walls. The density of each immunoreactive fiber tended to increase in the peripheral branch of the vascular tree. Retrograde axonal tracing with Fast Blue from the artery revealed that these immunoreactive fibers originated from NPY-containing catecholaminergic as well as VIP/SP/ CGRP-containing non-catecholaminergic neurons in the stellate ganglion and SP/ CGRP-containing neurons in the dorsal root ganglia of segments C, to Thl. After stellate ganglionectomy, TH-, NPY-, and, VIP-immunoreactive fibers disappeared completely from the arterial walls while approximately 40% of SP-and CGRPimmunoreactive fibers remained. The present results indicate that the artery of the dog forepaw receive triple innervation of adrenergic sympathetic, nonadrenergic sympathetic, and sensory fibers, and suggest that about 40% of SP-and CGRP-immunoreactive fibers are of sensory origin.

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Section: Discussionsupporting

confidence: 75%

Immunohistochemical Study of the Sympathetic and Sensory Innervation to the Blood Vessels of the Dog Forepaw.

Tsuruta

Masuko

Watanabe

1992

Tohoku J. Exp. Med.

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“…In peripheral nerves of aged animals both losses and degenerative changes have been reported for aminergic, cholinergic, and peptidergic nerve fibers (Cowen et al, 1982;Dhall et al, 1986;Mione et al, 1988;Cowen and Thrasivoulou, 1990;Navarro and Kennedy, 1990;AbdelRahman and Cowen, 1993). These data are consistent with morphological studies of peripheral sensory nerves and dorsal roots demonstrating axonal dystrophy, demyelination, axon degeneration, as well as loss of fibers during aging (Berg et al, 1962;van Steenis and Kroes, 1971;Samorajski, 1974;Burek et al, 1976;Sharma et al, 1980;Thomas et al, 1980;Cotard-Bartley et al, 1981;Mitsumori et al, 1981;Krinke, 1983).…”

Section: Discussionmentioning

confidence: 92%

Neuropeptides and neurotrophin receptor mRNAs primary sensory neurons of aged rats

Bergman

Johnson

et al. 1996

J. Comp. Neurol.

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Neuropeptides and neurotrophin receptors are regulated in primary sensory neurons in response to axonal injury, and axonal lesions are characteristic stigmata of aging primary sensory neurons. We have therefore examined the expression of neuropeptides and neurotrophin receptor mRNAs in 30-month-old (median survival age) Sprague-Dawley rats to see if similar adaptive mechanisms operate in senescence. The content of neuropeptides was examined with immunohistochemistry (IHC) and in situ hybridization (ISH), and the cellular mRNA expression of neurotrophin receptors was studied with ISH. All of the aged rats had symptoms of hind limb incapacity (posterior paralysis), but fore limbs did not seem affected. The size-distribution of neuronal profiles in cervical and lumbar dorsal root ganglia (DRGs) was similar in aged and young adult (2-3 months old) rats. In aged rats, the DRG neurons showed an increase in both immunolabelling and mRNA content of neuropeptide tyrosine (NPY), as well as an increased cellular expression of galanin mRNA. In the same animals, there were decreased cellular levels of calcitonin gene-related peptide (CGRP; IHC and ISH) and substance P (SP; IHC and ISH), while the difference in neuronal somatostatin (IHC and ISH) was small. The distribution of neuropeptide immunoreactivities in the dorsal horn of the corresponding spinal cord segments revealed a decreased labelling for CGRP-, SP-, and somatostatin-like immunoreactivities (LI) in the aged rats at both cervical and lumbar levels. NPY- and galanin-LI had a similar distribution in aged and young adult rats. NPY-immunoreactive fibers were also encountered in the dorsal column of aged but not young adult rats. ISH revealed that most of the primary sensory neurons express mRNA for the p75 low-affinity neurotrophin receptor (p75-LANR) and that there was no discernible difference between young adult and aged rats. The labelling intensity for mRNA encoding high-affinity tyrosine kinase receptors (TrkA, TrkB, and TrkC) was decreased in aged rat DRG neurons, while the percentage of neuronal profiles expressing mRNA for TrkA/B/C was similar in young adult and aged rats. The changed pattern of neuropeptide expression in primary sensory neurons of aged rats resembled that seen in young adult rats subjected to axonal injury of peripheral sensory nerves and may, thus, indicate aging-related lesions of sensory fibers. Since NPY is primarily present in large and galanin in small DRG neurons, the stronger effect on NPY as compared to galanin expression may indicate that aging preferentially affects neurons associated with mechanoreception (A alpha and A beta fibers) as compared to nociceptive units (A delta and C fibers). Furthermore, the observed changes in neuropeptide expression were most pronounced in lumbar DRGs, that harbors the sensory neurons supplying the affected hindlimbs of the rats.

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“…Results, however, are different between animal species and vascular tree [1], as well as in pathological conditions [2]. Except for an increased density of noradrenergic nervous fibers in the vasa nervorum [3], there is, sometimes, an age-related decrease of noradrenergic nerve fibers, for example, in the rabbit carotid artery [4] or in the guinea pig renal and femoral arteries [5] as well as in rat cerebral arteries [6] or in vascular smooth muscle of the human temporal artery [7]. The effect of age on rat coronary vessel sympathetic innervation was studied only by Santer [8] who, using the technique developed by Falck and Hillarp [9], did not find differences in the innervation of heart and coronary vessels in 24-month-old rats as compared with 4-monthInnervation of Rat Heart and Coronary Vessels old animals.…”

Section: Introductionmentioning

confidence: 99%

Age-Related Changes in Catecholaminergic Nerve Fibers of Rat Heart and Coronary Vessels

et al. 2003

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Background: Age-related changes of catecholaminergic nerve fibers supplying the myocardium and the coronary vessels were studied in adult and in old rats. Objective: The purpose of this study was to investigate whether there are quantitative changes in myocardial and/or coronary catecholaminergic nerve fibers and related neurotransmitters. Methods: Samples of myocardium and/or coronary vessels were studied by means of glyoxylic acid induced fluorescence, aldehyde-induced fluorescence, and neuropeptide Y immunoreactivity. All morphological results were submitted to quantitative analysis of images. Biochemical dosage of proteins and of norepinephrine was also performed, and morphological data were compared with biochemical ones. Finally, all data were subjected to statistical analysis. Results: Our findings are indicative of an age-related decrease of all types of fluorescent sympathetic nerve fibers in rat heart and coronary arteries. Moreover, our data show that aging induces a strong decrease of norepinephrine levels in tissue homogenates of rat heart and coronary vessels. The majority of the catecholaminergic nerve fibers that show strong age-related changes are not contiguous with contractile cells of myocardium and/or of vascular wall. In fact, these fibers are located in adventitial (or epicardial) and in endothelial (or endocardial) layers. Conclusion: The decrease of the catecholaminergic innervation of heart and coronary vessels of old rats can be in close relationships to the poor cardiac and/or coronary functions observed in old animals.

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Perivascular noradrenergic and peptide-containing nerves show different patterns of changes during development and ageing in the guinea-pig

Cited by 93 publications

References 31 publications

Immunohistochemical Study of the Sympathetic and Sensory Innervation to the Blood Vessels of the Dog Forepaw.

Immunohistochemical Study of the Sympathetic and Sensory Innervation to the Blood Vessels of the Dog Forepaw.

Neuropeptides and neurotrophin receptor mRNAs primary sensory neurons of aged rats

Age-Related Changes in Catecholaminergic Nerve Fibers of Rat Heart and Coronary Vessels

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