Perivenous Application of Fibrin Glue as External Support Enhanced Adventitial Adenovirus Transfection in Rabbit Model

Wan, Li; Li, Dianyuan; Wu, Qingyu

doi:10.1016/j.jss.2006.02.056

Cited by 16 publications

(15 citation statements)

References 14 publications

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“…22 However, studies on its use as a delivery scaffold for gene vectors are limited, and only adenovirus delivery has been investigated for the delivery of viral vectors. 26 FG has been investigated as a delivery scaffold for controlled release of adenovirus in a rabbit ear ulcer model 29 and for perivenous adventitial gene transfer, 30 and was shown to provide enhanced in vivo transgene expression over adenovirus transduction without FG scaffold. For cartilage tissue engineering purposes, FG scaffolds with nonviral copolymer-protected polyethylenimine-DNA vectors achieved sustained release of the gene over 20-day period and successful in vitro transfection of human keratinocytes and rabbit articular chondrocytes.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that diluted FG produces a more open fibrin network compared with undiluted FG scaffolds, 27 and that FG can act as an efficient scaffold for gene delivery. [28][29][30] In the current study, we investigated the effect of different fibrinogen dilutions during the preparation of FG scaffold on the delivery of AAV2 and their early effects on human BM-MSC (hBM-MSC) chondrogenesis in vitro. The aim of this study was to test the hypotheses that diluted FG scaffolds will release more viral particles, result in higher transduction efficiency, and increase the chondrogenic potential of transduced hBM-MSCs.…”

Section: Introductionmentioning

confidence: 99%

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Release of Bioactive Adeno-Associated Virus from Fibrin Scaffolds: Effects of Fibrin Glue Concentrations

Lee

Haleem

Yao

et al. 2011

Tissue Engineering Part A

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Fibrin glue (FG) is used in a variety of clinical applications and in the laboratory for localized and sustained release of factors potentially important for tissue engineering. However, the effect of different fibrinogen concentrations on FG scaffold delivery of bioactive adeno-associated viruses (AAVs) has not been established. This study was performed to test the hypothesis that FG concentration alters AAV release profiles, which affect AAV bioavailability. Gene transfer efficiency of AAV-GFP released from FG was measured using HEK-293 cells. Bioactivity of AAV transforming growth factor-beta1 (TGF-b 1 ) released from FG was assessed using the mink lung cell assay, and by measuring induction of cartilage-specific gene expression in human mesenchymal stem cells (hMSCs). Nondiluted FG had longer clotting times, smaller pore sizes, thicker fibers, and slower dissolution rate, resulting in reduced release of AAV. AAV release and gene transfer efficiency was higher with 25% and 50% FG than with the 75% and 100% FG. AAV-TGF-b 1 released from dilute-FG transduced hMSCs, resulting in higher concentrations of bioactive TGF-b 1 and greater upregulation of cartilage-specific gene expression compared with hMSC from undiluted FG. This study, showing improved release, transduction efficiency, and chondrogenic effect on hMSC of bioactive AAV-TGF-b 1 released from diluted FG, provides information important to optimization of this clinically available scaffold for therapeutic gene delivery, both in cartilage regeneration and for other tissue engineering applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Release of Bioactive Adeno-Associated Virus from Fibrin Scaffolds: Effects of Fibrin Glue Concentrations

Lee

Haleem

Yao

et al. 2011

Tissue Engineering Part A

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“…3 and 4) may lead to different fibrin glue concentrations within the vessel wall compartments and may exert different effects. Previous in vitro experiments have revealed that early distension could be diminished by external fibrin glue support [7,9,11] and in vivo experiments have furthermore revealed that perivenous support of vein grafts with fibrin glue can attenuate the severe injury encountered in the non-supported vein grafts exposed to arterial pressure at early time points after grafting [10]. However, at later time points it was demonstrated that external fibrin support showed negative or even detrimental effects on vein grafts [12].…”

Section: Discussionmentioning

confidence: 99%

“…These include the placement of a porous, non-restrictive, polyester stent [5][6][7], or a bio absorbable sheath [8] or the use of perivenous application of fibrin glue [9]. Perivenous fibrin glue has been used in a variety of experimental models and for different follow-up times [10][11][12]. The results of these studies were conflicting in terms of the development of intimal hyperplasia at different time points.…”

Section: Introductionmentioning

confidence: 99%

Extravascular perivenous fibrin support leads to aneurysmal degeneration and intimal hyperplasia in arterialized vein grafts in the rat

Stojanović

Ahmad

Didilis

et al. 2008

Langenbecks Arch Surg

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Background and aims External support of vein grafts by fibrin glue possibly prevents overdistension, vascular remodeling, and neointimal hyperplasia. Previous animal models of neointimal hyperplasia showed conflicting results. Here, long-term effects of external fibrin glue support were studied in a new rat model of jugular vein to abdominal aorta transposition. Materials and methods and methods In male Wistar rats (250-300 g) right jugular vein (1.0-1.5 cm) was transposed to the infrarenal aorta. Fibrin glue (0.25 ml) covered the vein before releasing the vascular clamps (n=6). Control vein grafts were exposed directly to blood pressure. After 16 weeks vein grafts were pressure-fixed for histology.

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“…We established a rabbit model of restenosis using the method described by Wan et al (2006a). After iv injection of heparin, the right common carotid artery and jugular vein were carefully exposed through a vertical midline neck incision.…”

Section: Establishing the Autologous Vein Graft Restenosis Modelmentioning

confidence: 99%

Apoptosis, proliferation, and morphology during vein graft remodeling in rabbits

et al. 2016

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ABSTRACT. Neo-intima development and atherosclerosis limit the long-term use of vein grafts for revascularization of ischemic tissues. Recently, studies have confirmed that proliferating cell nuclear antigen (PCNA) plays an important role in cell proliferation. Our research confirmed that 28 days after vein transplantation, PCNA expression increases significantly. Using rabbits, rather than rodents, for a more representative model of human vein grafts, we aimed to establish a time course of changes in cell proliferation and apoptosis using morphometric and immunohistochemical analyses, western blot, terminal deoxynucleotidyl transferase dUTP nick end labeling, and transmission electron microscopy (TEM). The external jugular veins of 42 healthy purebred male New Zealand white rabbits were grafted onto their common carotid arteries. The rabbits were divided into seven groups, with vein grafts being harvested before surgery, and at 1, 3, 7, 14, 28, and 90 days afterwards. The extent of stenosis and apoptosis, PCNA protein levels, and TEM morphology were subsequently examined. Intimal thickness was slightly decreased 1 day following surgery, but then increased continuously until the 90th day. Western blot and immunohistochemistry both indicated lowered PCNA expression on day 1, although levels subsequently increased, peaking at 7 days post-surgery. After surgery, apoptosis was lowest on day 7, and remained low thereafter. TEM revealed signs of apoptosis as vein graft restenosis progressed. Proliferation and apoptosis cooccurred following grafting, indicating that both processes were involved in vein graft remodeling. Apoptosis levels were highest between days 1 and 3 after surgery, whereas proliferation culminated on the 7th day.

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Perivenous Application of Fibrin Glue as External Support Enhanced Adventitial Adenovirus Transfection in Rabbit Model

Cited by 16 publications

References 14 publications

Release of Bioactive Adeno-Associated Virus from Fibrin Scaffolds: Effects of Fibrin Glue Concentrations

Release of Bioactive Adeno-Associated Virus from Fibrin Scaffolds: Effects of Fibrin Glue Concentrations

Extravascular perivenous fibrin support leads to aneurysmal degeneration and intimal hyperplasia in arterialized vein grafts in the rat

Apoptosis, proliferation, and morphology during vein graft remodeling in rabbits

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