PERK–KIPK–KCBP signalling negatively regulates root growth in Arabidopsis thaliana

Humphrey, Tania; Haasen, Katrina E.; Aldea-Brydges, May Grace; Sun, He; Zayed, Yara; Indriolo, Emily; Goring, Daphne R.

doi:10.1093/jxb/eru390

Cited by 45 publications

(72 citation statements)

References 54 publications

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“…To determine whether KCBP localizes to cortical MTs, we performed live-cell imaging using a functional, GFP-tagged KCBP fusion under the control of its endogenous regulatory elements; this construct fully rescued the typical zwichel trichome defects in the kcbp-1 (Salk_031704 in the Arabidopsis Biological Resource Center; see ‘Materials and methods’) mutant ( Figure 1—figure supplement 1A ) ( Humphrey et al, 2015 ), which was also designated as zwiA (N531704 in the Nottingham Arabidopsis Stock Centre; see ‘Materials and methods’) ( Buschmann et al, 2015 ). Notably, we found that GFP-KCBP localizes to puncta along cortical MTs in both pavement cells and hypocotyl cells ( Figure 1A , Figure 1—figure supplement 2B , Video 1 , Video 2 ).…”

Section: Resultsmentioning

confidence: 99%

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Orchestration of microtubules and the actin cytoskeleton in trichome cell shape determination by a plant-unique kinesin

Tian

Han

Feng

et al. 2015

eLife

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Microtubules (MTs) and actin filaments (F-actin) function cooperatively to regulate plant cell morphogenesis. However, the mechanisms underlying the crosstalk between these two cytoskeletal systems, particularly in cell shape control, remain largely unknown. In this study, we show that introduction of the MyTH4-FERM tandem into KCBP (kinesin-like calmodulin-binding protein) during evolution conferred novel functions. The MyTH4 domain and the FERM domain in the N-terminal tail of KCBP physically bind to MTs and F-actin, respectively. During trichome morphogenesis, KCBP distributes in a specific cortical gradient and concentrates at the branching sites and the apexes of elongating branches, which lack MTs but have cortical F-actin. Further, live-cell imaging and genetic analyses revealed that KCBP acts as a hub integrating MTs and actin filaments to assemble the required cytoskeletal configuration for the unique, polarized diffuse growth pattern during trichome cell morphogenesis. Our findings provide significant insights into the mechanisms underlying cytoskeletal regulation of cell shape determination.DOI: http://dx.doi.org/10.7554/eLife.09351.001

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Section: Resultsmentioning

confidence: 99%

“…A standard PCR-based method was used to identify the T-DNA insertion in kcbp-1 ( Humphrey et al, 2015 ), as described by Kong et al (2015) . Gene-specific primers (031704-LP and 031704-RP) were used to amplify an approximate 1000 bp DNA fragment in KCBP .…”

Section: Methodsmentioning

confidence: 99%

Orchestration of microtubules and the actin cytoskeleton in trichome cell shape determination by a plant-unique kinesin

Tian

Han

Feng

et al. 2015

eLife

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“…More than 300 CaMBPs are currently known, which include transporters and channels, metabolic enzymes, transcription factors, myosins, and various proteins of undefined functions that mostly interact with either holo-CaM or apo-CaM (Reddy et al, 2011). To date, only one CaM-binding MAP has been reported, KINESIN-LIKE CaM-BINDING PROTEIN/ ZWICHEL (KCBP/ZWI; Narasimhulu and Reddy, 1998), which functions in trichome development (Hülskamp et al, 1994;Oppenheimer et al, 1997;Tian et al, 2015) and root growth (Buschmann et al, 2015;Humphrey et al, 2015). During cell division, KCBP localizes to the cortical division zone and likely functions in phragmoplast guidance and in the spatial control of cytokinesis (Buschmann et al, 2015).…”

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confidence: 99%

The IQD Family of Calmodulin-Binding Proteins Links Calcium Signaling to Microtubules, Membrane Subdomains, and the Nucleus

et al. 2017

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ORCID IDs: 0000-0002-3493-4800 (K.B.); 0000-0002-7146-043X (B.M.).Calcium (Ca 2+ ) signaling and dynamic reorganization of the cytoskeleton are essential processes for the coordination and control of plant cell shape and cell growth. Calmodulin (CaM) and closely related calmodulin-like (CML) polypeptides are principal sensors of Ca 2+ signals. CaM/CMLs decode and relay information encrypted by the second messenger via differential interactions with a wide spectrum of targets to modulate their diverse biochemical activities. The plant-specific IQ67 DOMAIN (IQD) family emerged as possibly the largest class of CaM-interacting proteins with undefined molecular functions and biological roles. Here, we show that the 33 members of the IQD family in Arabidopsis (Arabidopsis thaliana) differentially localize, using green fluorescent protein (GFP)-tagged proteins, to multiple and distinct subcellular sites, including microtubule (MT) arrays, plasma membrane subdomains, and nuclear compartments. Intriguingly, the various IQD-specific localization patterns coincide with the subcellular patterns of IQD-dependent recruitment of CaM, suggesting that the diverse IQD members sequester Ca 2+ -CaM signaling modules to specific subcellular sites for precise regulation of Ca 2+ -dependent processes. Because MT localization is a hallmark of most IQD family members, we quantitatively analyzed GFP-labeled MT arrays in Nicotiana benthamiana cells transiently expressing GFP-IQD fusions and observed IQD-specific MT patterns, which point to a role of IQDs in MT organization and dynamics. Indeed, stable overexpression of select IQD proteins in Arabidopsis altered cellular MT orientation, cell shape, and organ morphology. Because IQDs share biochemical properties with scaffold proteins, we propose that IQD families provide an assortment of platform proteins for integrating CaM-dependent Ca 2+ signaling at multiple cellular sites to regulate cell function, shape, and growth.

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“…Similarly, several PP2Cs were found highly up-regulated in both quinoa genotypes (two PP2Cs) and exclusively in ST (four PP2Cs) under salt stress, indicating their negative regulation roles in ABA signaling pathway but positive roles in salt resistance. TIFY proteins, acting as repressors of JA responses, play an important role in plant development and response to abiotic stresses [68]. In the current study, one differentially expressed gene TIFY10A common in ST and SS was strongly induced in response to salt stress, which might protect quinoa against salt stress by regulating JA signaling pathway.…”

Section: Plant Hormone Signal Transduction Under Salt Stressmentioning

confidence: 55%

Transcriptome Analysis and Differential Gene Expression Profiling of Two Contrasting Quinoa Genotypes in Response to Salt Stress

Shi¹,

Gu²

2020

Preprint

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Background: Soil salinity is one of the major abiotic stress factors that affect crop growth and yield, which seriously restricts the sustainable development of agriculture. Quinoa is considered as one of the most promising crops in the future for its high nutrition value and strong adaptability to extreme weather and soil conditions. However, the molecular mechanisms underlying the adaptive response to salinity stress of quinoa remain poorly understood. To identify candidate genes related to salt tolerance, we performed reference-guided assembly and compared the gene expression in roots treated with 300 mM NaCl for 0, 0.5, 2, and 24 h of two contrasting quinoa genotypes differing in salt tolerance.Results: The salt-tolerant (ST) genotype displayed higher seed germination rate and plant survival rate, and stronger seedling growth potential as well than the salt-sensitive (SS) genotype under salt stress. An average of 38,510,203 high-quality clean reads were generated. Significant Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were identified to deeper understand the differential response. Transcriptome analysis indicated that salt-responsive genes in quinoa were mainly related to biosynthesis of secondary metabolites, alpha-Linolenic acid metabolism, plant hormone signal transduction, and metabolic pathways. Moreover, several pathways were significantly enriched amongst the differentially expressed genes (DEGs) in ST genotypes, such as phenylpropanoid biosynthesis, plant-pathogen interaction, isoquinoline alkaloid biosynthesis, and tyrosine metabolism. 117 DEGs were common to various stages of both genotypes, identified as core salt-responsive genes, including some transcription factor members, like MYB, WRKY and NAC, and some plant hormone signal transduction related genes, like PYL, PP2C and TIFY10A, which play an important role in the adaptation to salt conditions of this species. The expression patterns of 21 DEGs were detected by quantitative real-time PCR (qRT-PCR) and confirmed the reliability of the RNA-Seq results. Conclusions: We identified candidate genes involved in salt tolerance in quinoa, as well as some DEGs exclusively expressed in ST genotype. The DEGs common to both genotypes under salt stress may be the key genes for quinoa to adapt to salinity environment. These candidate genes regulate salt tolerance primarily by participating in reactive oxygen species (ROS) scavenging system, protein kinases biosynthesis, plant hormone signal transduction and other important biological processes. These findings provide theoretical basis for further understanding the regulation mechanism underlying salt tolerance network of quinoa, as well establish foundation for improving its tolerance to salinity in future breeding programs.

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PERK–KIPK–KCBP signalling negatively regulates root growth in Arabidopsis thaliana

Abstract: SummaryWe established an interaction network among three Arabidopsis PERK receptor-like kinases (8, 9, and 10), two AGC VIII kinases (AGC1-9 and KIPK) and KCBP with a role in negatively regulating root growth.

Cited by 45 publications

References 54 publications

Orchestration of microtubules and the actin cytoskeleton in trichome cell shape determination by a plant-unique kinesin

Orchestration of microtubules and the actin cytoskeleton in trichome cell shape determination by a plant-unique kinesin

The IQD Family of Calmodulin-Binding Proteins Links Calcium Signaling to Microtubules, Membrane Subdomains, and the Nucleus

Transcriptome Analysis and Differential Gene Expression Profiling of Two Contrasting Quinoa Genotypes in Response to Salt Stress

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