In the study carried out in Turkey in 2015, maize (Zea mays L. indentata), was cultivated by applying the drip irrigation system, with the aim of examining the effect of different irrigation applications on some anatomical features of the plant in detail. A period of 6 days was selected for irrigation interval and C2S1 quality irrigation water was used. Optimum condition (100%) and ecological conditions identified as ± 25% of the condition were created based on the Class A Pan method. For anatomical examination, the samples of maize were put in epon 812 and semithin sections were stained with toluidin blue. The semithin sections were examined with light microscopy and photographed. Ultrathins were examined with transmission electron microscopy (TEM) after staining with uranyl acetate and lead citrate, and their micrographs were taken. The vascular bundles surrounded the pith like a ring in the root. Very few stomata were observed in the stem. The pith was paranchymatic. The leaf was unifacial. The lower and the upper epidermis were amphistomatic and contained graminea stomate type. Numerous bullate cells with different sizes were observed in the epidermis. The mesophyll was formed by irregular cells due to stress under over-irrigation and limited irrigation conditions. The small vascular bundles were embedded in the mesophyll. The results were compared for two different ecological conditions by performing biometric measurements in some tissues in the root, stem and leaf. As a result, it was determined that maize cultivated under over-irrigation and limited irrigation conditions showed anatomical changes due to stress effect, adapted to the conditions and did not show abnormal structures compared to maize cultivated under optimum condition. Ecological condition created by reducing irrigation water by 25% in the cultivation of maize studied is anatomically convenient. The results obtained are significant for water saving in irrigation. However; morphological, physiological, etc. parameters of the plant should also be considered for an accurate suggestion.