Permeability and membrane sterol distribution in Saccharomyces uvarum and Kluyveromyces bulgaricus grown in presence of polyoxyalkylene glycol-oleic acid condensates

Pawiroharsono, Suyanto; Naji, B.; Bonaly, R.; Tonetti, F.; Chasseboeuf, C.; Richter, J

doi:10.1007/bf00251942

Cited by 12 publications

(10 citation statements)

References 22 publications

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“…This is consistent with an earlier study which suggested that antifoams can affect cell permeability in yeast by perturbing sterol biosynthesis which then alters the permeability of the membrane[45]. Yeast plasma membranes contain polar lipids such as glycerophospholipds and sphingolipids.…”

Section: How Do Antifoams Interact With Cells and Proteins In Bioprocsupporting

confidence: 92%

Beyond De-Foaming: The Effects of Antifoams on Bioprocess Productivity

Routledge

2012

Computational and Structural Biotechnology Journal

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Antifoams are often added to bioprocesses with little knowledge of their impact on the cells or product. However, it is known that certain antifoams can affect the growth rates of both prokaryotic and eukaryotic organisms in addition to changing surface properties such as lipid content, resulting in changes to permeability. This in turn can be beneficial to a recombinant protein production system for soluble proteins, as has been demonstrated by increased secretion of α-amylase and GFP, or achievement of greater yields of protein due to increased biomass. However, in some cases, certain concentrations of antifoams appear to have a detrimental effect upon cells and protein production, and the effects vary depending upon the protein being expressed. These findings emphasise the importance of optimising and understanding antifoam addition to bioprocesses.

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Section: How Do Antifoams Interact With Cells and Proteins In Bioprocsupporting

confidence: 92%

Beyond De-Foaming: The Effects of Antifoams on Bioprocess Productivity

Routledge

2012

Computational and Structural Biotechnology Journal

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“…Our findings suggest that antifoams can alter the permeability of yeast plasma membranes, which resulted in enhanced green fluorescent protein secretion [11]. These findings agree with a study suggesting that antifoams could alter sterol biosynthesis leading to changes in permeability [18], and is also in agreement with our preliminary electrospray mass spectrometry findings where we observed changes to relative phosphatidylcholine composition in 1% P2000 culture samples, in addition to each antifoam-containing sample having changes to relative phosphatidylinositol composition [Routledge SJ, Unpublished Data].…”

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confidence: 82%

News & Views in ... Pharmaceutical Bioprocessing

2014

Pharmaceutical Bioprocessing

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It is well known that foaming can become a major problem during large-scale recombinant protein bioprocesses; the vigorous stirring and introduction of gases to maintain the required dissolved oxygen (DO) concentration for the organism [1] in addition to the growth medium and protein products themselves [2], can result in the formation and stabilization of foams consisting of gas-filled liquid lamellas. Both unstable high liquid content foams and stable dry polyhedric foams may be present in a bioprocess [3] and readily accumulate. Unchecked build up of foams may result in foam escaping from the vessel, loss of sterility [4] and material, while bursting bubbles may damage proteins and cells [5] in addition to the blockage of exit filters which can pressurize the vessel and damage equipment.A common method of foam destruction is the addition of chemical antifoaming agents. Many types of antifoams are commercially available with a range of properties and varied foam destruction efficiency, with some becoming depleted requiring several additions over time [6]. They may contain surfactants and can consist of hydrophobic solids dispersed in carrier oil, aqueous suspensions or emulsions, liquid single components or solids [7]. Some examples of antifoams of a range of types are; Antifoams A and C (Sigma) which are both 30% emulsions of silicone polymer, J673A (Struktol) an alkoxylated fatty acid ester on a vegetable base, P2000 (Fluka) which is a polypropylene glycol, PEG600 and PEG8000 (Sigma) which are both polyethylene glycols, S184 (Wacker-Chemie Co.) which is a silicone oil, SB2121 (Struktol) a polyalkylene glycol, SE9 (Wacker-Chemie Co.) an emulsion containing 10% S184, and SLM54474 (Wacker-Chemie Co.) a polypropylene glycol. Most investigations concerning antifoams evaluate their defoaming capabilities; foam formation has been thoroughly characterized, and the main mechanisms of foam destruction have been explained, although some details of their action are not completely understood. This is partly due to submicroscopic events occurring during foam destruction and the complex involvement of the numerous active components of the agents [6], about which little information is generally provided by the manufacturer. Methods such as the Bartsch shaking test [8] and Ross-Miles pouring test [9] allow simple evaluation of antifoam efficiency and mathematical models have been generated allowing optimization of their addition to bioprocesses [6].Unexpectedly, considering their frequent use as an additive, there is significantly less information available concerning the biological effects of antifoaming agents and very little of this is recent. Present research has found that antifoams can have a broad range of effects upon bioprocesses, both on the culture environment and upon the cells themselves. The effects of these agents therefore warrant a more detailed evaluation. However, while many studies producing recombinant protein by large scale fermentation report the addition of antifoams, usually only the volume or concen...

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“…Previous work has shown that sterol abundance increased in Kluyveromyces bulgaricus and Saccharomyces uvarum under exposure to polyoxyalkylene glycol-oleic acid condensates in aerobic cultivations. The relative composition of sterols was unaffected in S. uvarum , while in K. bulgaricus , there was an increase in ergosterol ratio (Pawiroharsono et al 1987 ). The authors further stated that the permeability to certain sugars relevant for molasses fermentation, such as glucose and sucrose, was affected by the presence of the AFA investigated.…”

Section: Discussionmentioning

confidence: 99%

Industrial antifoam agents impair ethanol fermentation and induce stress responses in yeast cells

Nielsen

Lino²,

Rasmussen³

et al. 2017

Appl Microbiol Biotechnol

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The Brazilian sugarcane industry constitutes one of the biggest and most efficient ethanol production processes in the world. Brazilian ethanol production utilizes a unique process, which includes cell recycling, acid wash, and non-aseptic conditions. Process characteristics, such as extensive CO2 generation, poor quality of raw materials, and frequent contaminations, all lead to excessive foam formation during fermentations, which is treated with antifoam agents (AFA). In this study, we have investigated the impact of industrial AFA treatments on the physiology and transcriptome of the industrial ethanol strain Saccharomyces cerevisiae CAT-1. The investigated AFA included industrially used AFA acquired from Brazilian ethanol plants and commercially available AFA commonly used in the fermentation literature. In batch fermentations, it was shown that industrial AFA compromised growth rates and glucose uptake rates, while commercial AFA had no effect in concentrations relevant for defoaming purposes. Industrial AFA were further tested in laboratory scale simulations of the Brazilian ethanol production process and proved to decrease cell viability compared to the control, and the effects were intensified with increasing AFA concentrations and exposure time. Transcriptome analysis showed that AFA treatments induced additional stress responses in yeast cells compared to the control, shown by an up-regulation of stress-specific genes and a down-regulation of lipid biosynthesis, especially ergosterol. By documenting the detrimental effects associated with chemical AFA, we highlight the importance of developing innocuous systems for foam control in industrial fermentation processes.Electronic supplementary materialThe online version of this article (10.1007/s00253-017-8548-2) contains supplementary material, which is available to authorized users.

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Permeability and membrane sterol distribution in Saccharomyces uvarum and Kluyveromyces bulgaricus grown in presence of polyoxyalkylene glycol-oleic acid condensates

Cited by 12 publications

References 22 publications

Beyond De-Foaming: The Effects of Antifoams on Bioprocess Productivity

Beyond De-Foaming: The Effects of Antifoams on Bioprocess Productivity

News & Views in ... Pharmaceutical Bioprocessing

Industrial antifoam agents impair ethanol fermentation and induce stress responses in yeast cells

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