Abstract. Cuticular membranes were isolated enzymically from tomato fruits and from the dorsal and ventral surfaces of the leaves of Euonymus japonicus. Penetration of Fe from in the absence and presence of urea through the isolated cuticular membranes was studied. Fe from FeSO4 penetrated more rapidly through the cuticles than Fe from FeEDDHA. Urea reduced the penetration of Fe from FeSO4 and FeEDDHA. Binding of Fe on the inner surfaces of tomato fruit cuticles was also reduced by EDDHA.The results on the rates of penetration of 14C-labeled organic substances suggest a possible relationship with their molecular weights. Permeability coefficients for these solutes have been derived for the 3 types of cuticles, using Fick's equation. The penetration rates of all substances through stomatous cuticles far exceeded those through astomatous cutieles.Foliar absorption of nutrients is a multistep process (14). Adsorption on the surface of, and penetration of ions through the cuticle which covers the entire leaf surface are prerequisites (20,24) to active uptake of ions by leaf cells (12, 15). Increasingly greater attention has been directed toward the importance of this common barrier to foliar entry of solutes. Although the morphology, physiology and biochemistry of plant cuticles have been discussed ( 1, 4, 5, 16), the mode of entry of nutrient ions and other chemicals through the cuticles is not vet understood. Cuticular membranes have been isolated enzymically from ripe tomato fruits and onion leaves and their permeability, surface binding an-d ion exchange properties with respect to rubidium, calcium and urea have been studied (23,24,25). Urea penetrated the cuticular membranes at a remarkable rate and also enhanced the penetration of other ions (25).The permeability of enzymically isolated cuticular memibranes to 59Fe-labeled FeSO4 and FeEDDHA (ferric ethylenediamine di (o-hydroxyphenylacetate) in the absence and presence of urea, and also to 14C-labeled organic substances of different molecular weights are reported herein. The effects of EDDHA on Fe binding by the inner surface of tomato fruit cuticles were also studied.