Permeability of Azotobacter to Succinate and Malate,

Repaske, Roy; Shroat, Joan; Allman, D.W.

doi:10.1128/jb.79.3.394-405.1960

Cited by 8 publications

(2 citation statements)

References 36 publications

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“…This was not expected as, e.g. malate and acetate are known to be oxidized by Azotobacter species (Repaske et al ., 1960). The DGGE analysis of nifH‐g1 PCR products of the citric and malic acid treatments could indicate that some potential diazotrophs increased their population.…”

Section: Discussionmentioning

confidence: 92%

Effects of model root exudates on structure and activity of a soil diazotroph community

Bürgmann

Meier

Bunge

et al. 2005

Environmental Microbiology

167

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SummaryNitrogen fixation is often enhanced in the rhizosphere as compared with bulk soil, due to asymbiotic microorganisms utilizing root exudates as an energy source. We have studied the activity and composition of asymbiotic soil diazotrophs following pulse additions of artificial root exudates and single carbon sources, simulating the situation of bulk soil coming into contact with exudates from growing roots. Artificial root exudates and single sugars rapidly induced nitrogen fixation. The population of potential diazotrophs was studied using universal and group-specific nifH polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) analysis. Reverse transcription PCR of nifH mRNA confirmed that phylotypes with an apparently increasing population size also expressed the nitrogenase system. According to our results, the actively nitrogen-fixing population represents only a fraction of the total diazotroph diversity, and the results of group-specific nifH PCR and phylogenetic analysis of cloned nifH and 16S rRNA gene fragments identified active species that belonged to the genus Azotobacter . Rapid changes of transcriptional activity over time were observed, indicating different growth and activation strategies in different Azotobacter strains. Only sugar-containing substrates were able to induce nitrogen fixation, but substrate concentration and the presence of organic acids may have additional selective effects on the active diazotroph population.

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Section: Discussionmentioning

confidence: 92%

Effects of model root exudates on structure and activity of a soil diazotroph community

Bürgmann

Meier

Bunge

et al. 2005

Environmental Microbiology

167

View full text Add to dashboard Cite

show abstract

“…The phenomena here described have some unusual features, however. Repaske et al (10) have shown that the lag period in respiration of Azotobacter agilis incubated with succinate or malate is reduced by the addition of glucose, fructose, or acetate. The optimal effect was achieved by relatively small amounts of the substrates.…”

Section: Discussionmentioning

confidence: 99%

Catabolic Activities of Neisseria meningitidis: Utilization of Succinate

Mallavia

Weiss

1970

J Bacteriol

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When resting cells of Neisseria meningitidis group B were incubated with either succinate, fumarate, or malate, respiration and CO2 production were not significantly stimulated. These dicarboxylic acids were readily utilized, however, when they were added in association with a combination of a-ketoglutarate and aspartate or with glucose or with glutamate. The amounts of these substrates required for exogenous succinate utilization were relatively large. Both the a-ketoglutarate-aspartate combination and glutamate greatly stimulated succinate uptake into the cells, but glutamate was far more effective than the a-ketoglutarate-aspartate combination in eliciting exogenous succinate utilization. This difference is explained on the basis of evidence reported in another article that succinate derived from the aketoglutarate-aspartate mixture is metabolized more rapidly-and thus more rapidly dilutes the specific activity of added succinate-than the succinate derived from glutamate.

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The inducible transport of di- and tricarboxylic acid anions across the membrane of Azotobacter vinelandii

Postma

Dam

1971

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Permeability of Azotobacter to Succinate and Malate,

Cited by 8 publications

References 36 publications

Effects of model root exudates on structure and activity of a soil diazotroph community

Effects of model root exudates on structure and activity of a soil diazotroph community

Catabolic Activities of Neisseria meningitidis: Utilization of Succinate

The inducible transport of di- and tricarboxylic acid anions across the membrane of Azotobacter vinelandii

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