Permeabilization of ultraviolet-irradiated Chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus.

Abstract

Chinese hamster V-79 cells were made permeable by treatment with polyethylene glycol and then incubated with a Micrococcus luteus extract containing ultraviolet-specific endonuclease activity. This treatment introduced nicks in irradiated, but not in unirradiated, deoxyribonucleic acid. The nicks remained open for at least 3 h; there was no loss of endonuclease-sensitive sites, and no excision of dimers as measured by chromatography was detected. In addition, there was no increase in ultraviolet resistance in treated cells. This suggests that the absence of a significant amount of excision repair in rodent cells is due to the lack of both incision and excision capacity.

UV Protective Effects of DNA Repair Enzymes and RNA Lotion

“…We aimed to determine the effects of two topical formulations containing (1) DNA repair enzymes ( Micrococcus luteus ) (18–20), or (2) fragments of UVC‐irradiated rabbit globin mRNA (17,21), on UV‐induced contact hypersensitivity (CHS) suppression in human subjects. A corollary aim was to measure the effects of these agents on levels of thymine dimers in skin biopsies obtained after simulated solar radiation (SSR).…”

Permeabilization of lymphocytes with polyethylene glycol 1000. Discrimination of permeabilized cells by flow cytometry

“…This study was initiated AND TRON to examine whether the toxic effects of PEG treatment, which are frequently encountered in cell fusion experiments and noticed by dye exclusion tests, may be utilized as alternative to other methods of cell permeabilization (9,22). A similar approach has been described recently (23).…”