Permeation mechanism of DNA molecules in solution through cuprammonium regenerated cellulose hollow fiber (BMMtm)

Hirasaki, Tomoko; Sato, Tetsuo; Tsuboi, Takashi; Nakano, Hiroo; Noda, Tokiti; Kono, Akira; Yamaguchi, Kazuhito; Imada, Kenjiro; Yamamoto, Naoki; Murakami, Hiroki; Manabe, Sei-ichi

doi:10.1016/0376-7388(95)00082-n

Cited by 24 publications

(12 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hadidi et al () reported that large polysaccharide molecules can be transmitted through UF membranes rated as having a nominal molecular weight cut off (MWCO) over two times lower than that for the polysaccharide molecule. Similar behavior has been reported in studies that investigated the filtration behavior of large DNA molecules (Arkhangelsky et al, ; Hirasaki et al, ; Latulippe et al, ). Whereas the extent of polysaccharide transmission was explained according to concentration polarization effects, the DNA transmission was found to be due elongation/deformation effects at the entrance of the membrane pore.…”

Section: Introductionsupporting

confidence: 82%

Optimization of biomolecule separation by combining microscale filtration and design‐of‐experiment methods

Kazemi

Kawka

Latulippe

2016

Biotech & Bioengineering

View full text Add to dashboard Cite

There is considerable interest in developing microscale (i.e., high-throughput) methods that enable multiple filtration experiments to be run in parallel with smaller sample amounts and thus reduce the overall required time and associated cost to run the filtration tests. Previous studies to date have focused on simply evaluating the filtration capacity, not the separation performance. In this work, the stirred-well filtration (SWF) method was used in combination with design-of-experiment (DOE) methods to optimize the separation performance for three binary mixtures of bio-molecules: protein-protein, protein-polysaccharide, and protein-DNA. Using the parallel based format of the SWF method, eight constant-flux ultrafiltration experiments were conducted at once to study the effects of stirring conditions, permeate flux, and/or solution conditions (pH, ionic strength). Four separate filtration tests were conducted for each combination of process variables; in total, over 100 separate tests were conducted. The sieving coefficient and selectivity results are presented to match the DOE design format and enable a greater understanding of the effects of the different process variables that were studied. The method described herein can be used to rapidly determine the optimal combination of process factors that give the best separation performance for a range of membrane-based separations applications and thus obviate the need to run a large number of traditional lab-scale tests. Biotechnol. Bioeng. 2016;113: 2131-2139. © 2016 Wiley Periodicals, Inc.

show abstract

Section: Introductionsupporting

confidence: 82%

Optimization of biomolecule separation by combining microscale filtration and design‐of‐experiment methods

Kazemi

Kawka

Latulippe

2016

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…7). This indicates that the content of DNA molecules in the filtrates can be assessed by semi-quantitative PCR if the appropriate amplification cycle is adopted, and that the PCR result accurately reflects the removability of DNA molecules by BMMs as previously described [30,31].…”

Section: Discussionmentioning

confidence: 99%

Electron microscopic estimation of removal of parvovirus B19 (HPVB19) by nanofiltration with a novel filter membrane

Yamaguchi

Miyagawa

Takahashi³

et al. 2007

Journal of Membrane Science

Self Cite

View full text Add to dashboard Cite

“…Hirasaki et al [7] examined DNA filtration through membranes with mean pore diameters of 15 and 35 nm that were originally developed for virus filtration. DNA transmission decreased with increasing size of the DNA, with the fractional transmission for the DNA being significantly larger than that for globular proteins of comparable molecular weight.…”

Section: Introductionmentioning

confidence: 99%

Flux-dependent transmission of supercoiled plasmid DNA through ultrafiltration membranes

Latulippe

Ager

Zydney

2007

Journal of Membrane Science

View full text Add to dashboard Cite

Permeation mechanism of DNA molecules in solution through cuprammonium regenerated cellulose hollow fiber (BMMtm)

Cited by 24 publications

References 14 publications

Optimization of biomolecule separation by combining microscale filtration and design‐of‐experiment methods

Optimization of biomolecule separation by combining microscale filtration and design‐of‐experiment methods

Electron microscopic estimation of removal of parvovirus B19 (HPVB19) by nanofiltration with a novel filter membrane

Flux-dependent transmission of supercoiled plasmid DNA through ultrafiltration membranes

Contact Info

Product

Resources

About