Permeation of Antimicrobial Agents through &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; Biofilms: A Simple Method

Shigeta, Masanobu; Tanaka, Genki; Komatsuzawa, Hitoshi; Sugai, Motoyuki; Suginaka, Hidekazu; Usui, Tsuguru

doi:10.1159/000239587

Cited by 168 publications

(121 citation statements)

References 10 publications

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“…Slow growth undoubtedly contributes to resistance to killing by antimicrobials, but this is not a unique feature of biofilm cells. With the exception of that of aminoglycosides (17,19,26,33), the exopolysaccharide matrix has not been found to notably retard the diffusion of antibiotics. For example, fluoroquinolones readily penetrate the biofilm (1,19,33,37).…”

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confidence: 96%

Biofilms and Planktonic Cells of Pseudomonas aeruginosa Have Similar Resistance to Killing by Antimicrobials

2001

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Biofilms are considered to be highly resistant to antimicrobial agents. Strictly speaking, this is not the case-biofilms do not grow in the presence of antimicrobials any better than do planktonic cells. Biofilms are indeed highly resistant to killing by bactericidal antimicrobials, compared to logarithmic-phase planktonic cells, and therefore exhibit tolerance. It is assumed that biofilms are also significantly more tolerant than stationary-phase planktonic cells. A detailed comparative examination of tolerance of biofilms versus stationary-and logarithmic-phase planktonic cells with four different antimicrobial agents was performed in this study. Carbenicillin appeared to be completely ineffective against both stationary-phase cells and biofilms. Killing by this ␤-lactam antibiotic depends on rapid growth, and this result confirms the notion of slowgrowing biofilms resembling the stationary state. Ofloxacin is a fluoroquinolone antibiotic that kills nongrowing cells, and biofilms and stationary-phase cells were comparably tolerant to this antibiotic. The majority of cells in both populations were eradicated at low levels of ofloxacin, leaving a fraction of essentially invulnerable persisters. The bulk of the population in both biofilm and stationary-phase cultures was tolerant to tobramycin. At very high tobramycin concentrations, a fraction of persister cells became apparent in stationary-phase culture. Stationary-phase cells were more tolerant to the biocide peracetic acid than were biofilms. In general, stationary-phase cells were somewhat more tolerant than biofilms in all of the cases examined. We concluded that, at least for Pseudomonas aeruginosa, one of the model organisms for biofilm studies, the notion that biofilms have greater resistance than do planktonic cells is unwarranted. We further suggest that tolerance to antibiotics in stationary-phase or biofilm cultures is largely dependent on the presence of persister cells.

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confidence: 96%

Biofilms and Planktonic Cells of Pseudomonas aeruginosa Have Similar Resistance to Killing by Antimicrobials

2001

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“…Sometimes, if the antibiotic gets degraded while penetrating the biofilm, the antibiotic action declines rapidly. Antibiotics may get adsorbed on the extracellular polymeric surfaces of the biofilm which can reduce the penetration of the antibiotic (aminoglycosides) (Shigeta et al, 1997). Sometimes, antibiotics which are positively charged in nature can bind to the negatively charged molecules of the biofilm matrix.…”

Section: Biofilm and Drug Resistancementioning

confidence: 99%

Review on biofilm formation and its control options

2017

Int. J. Adv. Res. Biol. Sci

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The generalized idea that bacteria have a unicellular way of life is not entirely accurate, given that pure planktonic growth is uncommon. Biofilms are defined as an organized group of microorganisms living within a self-produced matrix of polymeric substances which gets attached to several surfaces. This review was done with the objective to give an overview on the mechanism of biofilm formation and highlight its veterinary and public health implications and control options. The formation of a biofilm occurs in five stages, stage of cell attachment, stage securely affixing of cells, stage of micro-colonies formation and beginning to mature, Stage of more maturation, and stage of dispersal of cells from the biofilm. Factors controlling cell attachment include nature of surface, properties of medium and properties of the microbial cell surface. Formation of a biofilm is dependent on the interaction between the environmental stimuli and the reciprocation of the corresponding signalling events by the microorganisms. Biofilms are composed primarily of microbial cells and EPS. The importance of biofilm in disease processes in humans and animals is now widely recognized. In animal species, the risk of infection is probably greater than the risk in humans. In human infections associated with biofilm formation were medical device-related infections including Pacemakers, electrical dialysers, joint prosthetics, intravenous catheters, urinary catheter. As food is identified to be a very efficient vehicle for bringing a large number of people into contact with a potential hazard, food processing equipments can be persistent source of spoilage and pathogenic bacteria if microorganisms form biofilm on them. Ideally, preventing biofilm formation would be a more logical option than treating it. The main strategy to prevent biofilm formation is to clean and disinfect regularly before bacteria attach firmly to surfaces. This process can remove 90% or more of microorganisms associated with the surface. Biofilm detectors, acid shock treatment and recently using bacteriophages have been tried. The prevention and control of biofilm formation is somewhat difficult but prevention is the ideal approach even if there are control methods too. The development of better control and prevention methods with better effect need to be given special emphasis.

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“…Nowadays, the research methodology of antibiotic penetration analysis into bacterial biofilms is based on bioassay analysis [2], analysis of antibiotic penetration rates through the biofilm formed on cell culture inserts [5] or analysis of IR bands of the biofilm in regions of spectrum associated with nucleic acids in the presence of antibiotics (ATR/FT-IR) [6].…”

Section: Introductionmentioning

confidence: 99%

Laser Interferometry Analysis of Ciprofloxacin Diffusion through Pseudomonas aeruginosa Biofilm

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2012

Clin Microbial

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Permeation of Antimicrobial Agents through <i>Pseudomonas aeruginosa</i> Biofilms: A Simple Method

Cited by 168 publications

References 10 publications

Biofilms and Planktonic Cells of Pseudomonas aeruginosa Have Similar Resistance to Killing by Antimicrobials

Biofilms and Planktonic Cells of Pseudomonas aeruginosa Have Similar Resistance to Killing by Antimicrobials

Review on biofilm formation and its control options

Laser Interferometry Analysis of Ciprofloxacin Diffusion through Pseudomonas aeruginosa Biofilm

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