Permeation of several cryoprotectant agents into porcine articular cartilage

Jomha, Nadr M.; Law, Garson K.; Abazari, Alireza; Rekieh, Kassim; Elliott, Janet A.W.; McGann, Locksley E.

doi:10.1016/j.cryobiol.2008.11.004

Cited by 47 publications

(32 citation statements)

References 19 publications

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“…Jomha et al 22 compared the permeation of 4 cryoprotective agents in osteochondral porcine dowels at different temperatures and different incubation times. They concluded that a combination of different cryoprotective agents could be helpful because they can be used at nontoxic concentrations.…”

Section: Large Fresh Osteochondral Allograft Of Kneementioning

confidence: 99%

See 1 more Smart Citation

Large Fresh Osteochondral Allografts of the Knee: A Systematic Clinical and Basic Science Review of the Literature

Bisicchia

Amendola

et al. 2015

Arthroscopy: The Journal of Arthroscopic & Related Surgery

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Section: Large Fresh Osteochondral Allograft Of Kneementioning

confidence: 99%

“…[15][16][17][18][19][20][21][22][23][24][25][26][27][28] Cellular viability has always been considered one of the most important factors for successful osteochondral allograft transplantation. The effects of different storage times and different media on cellular viability have been widely studied in the past.…”

Section: Basic Science Reviewmentioning

confidence: 99%

Large Fresh Osteochondral Allografts of the Knee: A Systematic Clinical and Basic Science Review of the Literature

Bisicchia

Amendola

et al. 2015

Arthroscopy: The Journal of Arthroscopic & Related Surgery

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“…Investigation of Me 2 SO permeation into AC has been studied with ovine (Pegg et al, 2006b), porcine (Muldrew et al, 1996;Sharma et al, 2007;Jomha et al, 2009), bovine (Mukherjee et al, 2008), and human AC (Carsi et al, 2004). In these studies, the temperature levels were between approximately 0 and 37 °C.…”

Section: Resultsmentioning

confidence: 99%

“…Due to the importance of the CPA permeation characteristics to the cryopreservation of tissues, many methods have been developed to investigate the penetration rates of CPA in tissue samples, such as using nuclear magnetic resonance (NMR) techniques (Muldrew et al, 1996;Carsi et al, 2004;Mukherjee et al, 2008), high performance liquid chromatography (HPLC) (Carpenter and Dawson, 1991;Hu and Wolfinbarger, 1994;Pegg, 2006), radiotracer ( 35 S) (Elford, 1970), osmometer (Sharma et al, 2007;Jomha et al, 2009;Yu et al, 2010), conductivity meter (Chen et al, 2009), or electronic microbalance (Pegg, 2006). In this study, the quantity of Me 2 SO in ovine AC was measured using a spectrophotometer.…”

Section: Resultsmentioning

confidence: 99%

“…Mukherjee et al (2008) studied the diffusions of high concentration single CPA [6.9 mol/L, dimethyl sulfoxide (Me 2 SO)] and multi-component CPA mixture (VS55, 3.1 mol/L Me 2 SO+2.2 mol/L 1,2-propanediol+3.1 mol/L formamide) in immature bovine AC. Sharma et al (2007) and Jomha et al (2009) examined the time-dependent permeations of Me 2 SO, propylene glycol (PG), ethylene glycol (EG), and glycerol into intact porcine AC at concentrations suitable for vitrification. Carsi et al (2004) determined the diffusion coefficients of Me 2 SO and glycerol in human AC at different temperatures and concentrations.…”

Section: Introductionmentioning

confidence: 99%

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Permeation of dimethyl sulfoxide into articular cartilage at subzero temperatures

Zhang

Chen

2012

J. Zhejiang Univ. Sci. B

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Abstract:Osteochondral allografting has been proved to be a useful method to treat diseased or damaged areas of joint surfaces. Operational long-term stocks of grafts which supply a buffer between procurement and utilization would contribute to the commercialization or industrialization of this technology. Vitrification has been thought to be a promising method for successful preservation of articular cartilage (AC), but high concentration cryoprotectants (CPAs) are used which may cause high cellular toxicity. An effective way to reduce CPA toxicity is to increase CPA concentration gradually while the temperature is lowered. Understanding the mechanism of CPA permeation at subzero temperatures is important for designing the cryopreservation protocol. In this research, the permeation of dimethyl sulfoxide (Me 2 SO) in ovine AC at subzero temperatures was studied experimentally. Pretreated AC discs were exposed in Me 2 SO solutions for different time (0, 5, 15, 30, 50, 80, and 120 min) at three temperature levels (−10, −20, and −30 °C). The Me 2 SO concentration within the tissue was determined by ultraviolet (UV) spectrophotometry. The diffusion coefficients were estimated to be 0.85×10 −6 , 0.48×10 −6 , and 0.27×10 −6 cm 2 /s at −10, −20, and −30 °C, respectively, and the corresponding activation energy was 29.23 kJ/mol. Numerical simulation was performed to compare two Me 2 SO addition protocols, and the results demonstrated that the total loading duration could be effectively reduced with the knowledge of permeation kinetics.

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Diffusion Limited Cryopreservation of Tissue with Radiofrequency Heated Metal Forms

Han

Sharma

Gao

et al. 2020

Adv Healthcare Materials

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Cryopreserved tissues are increasingly needed in biomedical applications. However, successful cryopreservation is generally only reported for thin tissues (≤1 mm). This work presents several innovations to reduce cryoprotectant (CPA) toxicity and improve tissue cryopreservation, including 1) improved tissue warming rates through radiofrequency metal form and field optimization and 2) an experimentally verified predictive model to optimize CPA loading and rewarming to reduce toxicity. CPA loading is studied by microcomputed tomography (µCT) imaging, rewarming by thermal measurements, and modeling, and viability is measured after loading and/or cryopreservation by alamarBlue and histology. Loading conditions for three common CPA cocktails (6, 8.4, and 9.3 m) are designed, and then fast cooling and metal forms rewarming (up to 2000°C min −1) achieve ≥90% viability in cryopreserved 1-2 mm arteries with various CPAs. Despite high viability by alamarBlue, histology shows subtle changes after cryopreservation suggesting some degree of cell damage especially in the central portions of thicker arteries up to 2 mm. While further studies are needed, these results show careful CPA loading and higher metal forms warming rates can help reduce CPA loading toxicity and improve outcomes from cryopreservation in tissues while also offering new protocols to preserve larger tissues ≥1 mm in thickness.

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Permeation of several cryoprotectant agents into porcine articular cartilage

Cited by 47 publications

References 19 publications

Large Fresh Osteochondral Allografts of the Knee: A Systematic Clinical and Basic Science Review of the Literature

Large Fresh Osteochondral Allografts of the Knee: A Systematic Clinical and Basic Science Review of the Literature

Permeation of dimethyl sulfoxide into articular cartilage at subzero temperatures

Diffusion Limited Cryopreservation of Tissue with Radiofrequency Heated Metal Forms

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