Peronospora-like fungi (Chromista, Peronosporales) parasitic on Brassicaceae and related hosts

Constantinescu, O.; Fatehi, Jamshid

doi:10.1127/0029-5035/2002/0074-0291

Cited by 121 publications

(62 citation statements)

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“…The obligate biotrophic oomycete Hyaloperonospora parasitica (formerly Peronospora parasitica [Constantinescu and Fatehi, 2002]) causes downy mildew of the model plant Arabidopsis thaliana. Arabidopsis accessions that are resistant to H. parasitica carry Recognition of Peronospora parasitica (RPP) resistance genes.…”

Section: Introductionmentioning

confidence: 99%

Differential Recognition of Highly Divergent Downy Mildew Avirulence Gene Alleles by RPP1 Resistance Genes from Two Arabidopsis Lines

et al. 2005

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The perception of downy mildew avirulence (Arabidopsis thaliana Recognized [ATR]) gene products by matching Arabidopsis thaliana resistance (Recognition of Peronospora parasitica [RPP]) gene products triggers localized cell death (a hypersensitive response) in the host plant, and this inhibits pathogen development. The oomycete pathogen, therefore, is under selection pressure to alter the form of these gene products to prevent detection. That the pathogen maintains these genes indicates that they play a positive role in pathogen survival. Despite significant progress in cloning plant RPP genes and characterizing essential plant components of resistance signaling pathways, little progress has been made in identifying the oomycete molecules that trigger them. Concluding a map-based cloning effort, we have identified an avirulence gene, ATR1 NdWsB , that is detected by RPP1 from the Arabidopsis accession Niederzenz in the cytoplasm of host plant cells. We report the cloning of six highly divergent alleles of ATR1 NdWsB from eight downy mildew isolates and demonstrate that the ATR1 NdWsB alleles are differentially recognized by RPP1 genes from two Arabidopsis accessions (Niederzenz and Wassilewskija). RPP1-Nd recognizes a single allele of ATR1 NdWsB ; RPP1-WsB also detects this allele plus three additional alleles with divergent sequences. The Emco5 isolate expresses an allele of ATR1 NdWsB that is recognized by RPP1-WsB, but the isolate evades detection in planta. Although the Cala2 isolate is recognized by RPP1-WsA, the ATR1 NdWsB allele from Cala2 is not, demonstrating that RPP1-WsA detects a novel ATR gene product. Cloning of ATR1 NdWsB has highlighted the presence of a highly conserved novel amino acid motif in avirulence proteins from three different oomycetes. The presence of the motif in additional secreted proteins from plant pathogenic oomycetes and its similarity to a host-targeting signal from malaria parasites suggest a conserved role in pathogenicity.

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Section: Introductionmentioning

confidence: 99%

Differential Recognition of Highly Divergent Downy Mildew Avirulence Gene Alleles by RPP1 Resistance Genes from Two Arabidopsis Lines

et al. 2005

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“…For example, mutations in one of the defense regulators discovered in Arabidopsis, designated EDS1 (enhanced disease susceptibility), exhibit broad spectrum susceptibility to several pathogens including isolates of Albugo candida (white rust) (Figure 1) and Hyaloperonospora parasitica (downy mildew) (CONSTANTINESCU & FATEHI 2002) from Brassica oleracea (PARKER et al 1996). Isolates of both pathogens collected from either brassica or Arabidopsis represent distinct subspecies, based on their restrictive host specialization and molecular divergence (REHMANY et al 2000).…”

Section: Species Level ("Non-host") Disease Resistancementioning

confidence: 99%

Genetics of disease resistance in Arabidopsis to crop pathogens

Holub¹,

Tör²,

Cooper³

et al. 2002

Plant Prot. Sci.

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Arabidopsis is universally resistant as a species to many crop pathogens, including examples from other crucifers such as Albugo candida and Hyaloperonospora parasitica from Brassica oleracea. This species level trait could potentially provide a source of durable disease resistance in crops if examples can be found which are amenable to molecular genetic characterization. Our research has developed from the observation that null mutation in Arabidopsis of a defense regulatory gene EDS1 (enhanced disease susceptibility) is susceptible to isolates of A. candida and H. parasitica from brassica. EDS1 is required by a major structural class of R-genes to confer resistance in Arabidopsis. We have therefore focused on identifying R-genes in Arabidopsis that are responsible for conferring resistance to brassica pathogens.

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“…It has been estimated that combined infection of leaf and inflorescence can cause up to 63% yield loss with more severe losses (90%) as a result of staghead formation in susceptible cultivars (Lakra and Saharan 1989). Moreover, A. candida has the capacity under field conditions to elevate the incidence and severity of infection by Hyaloperonospora parasitica (Brassica downy mildew) in crucifers including B. juncea (Sansome and Sansome 1974;Chaurasia et al 1982;Bains and Jhooty 1985;Constantinescu and Fatehi 2002). Almost all the major varieties of B. juncea belonging to the Indian gene pool are highly susceptible to the white rust pathogen (Panjabi et al 2010).…”

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confidence: 99%

Validation of molecular markers for marker-assisted pyramiding of white rust resistance loci in Indian Mustard (Brassica juncea L.)

et al. 2015

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. 2015. Validation of molecular markers for marker-assisted pyramiding of white rust resistance loci in Indian Mustard (Brassica juncea L.). Can. J. Plant Sci. 95: 939Á945. Successful application of molecular markers in markerassisted pyramiding relies on effective determination of the target phenotype. In this respect, evaluation of the efficiency of markers for marker-assisted selection through cross-validation in different genetic backgrounds and in different populations is a crucial step. In the present study, the previously identified Arabidopsis-derived intron polymorphic (IP) markers At5g41560 and At2g36360, which were highly linked with AcB1-A4.1 and AcB1-A5.1, respectively, were validated in a set of 25 genotypes of Indian Mustard and in three different F 2 populations. The relationships between the variation of PCR products of the two markers with the percent disease index (PDI) of the tested genotypes, and the co-segregation analysis of the markers with disease phenotype in F 2 populations clearly indicated that At5g41560 and At2g36360 are genotype-nonspecific markers and are closely linked to white rust resistance loci AcB1-A4.1 and AcB1-A5.1, respectively. It also became evident from the present study that AcB1-A4.1 and an another white rust resistance locus Ac(2)t are likely the same gene locus.Key words: White rust, marker-assisted selection, marker validation Singh, B. K., Nandan, D., Supriya, A., Ram, B., Kumar, A., Singh, T., Meena, H. S., Kumar, V., Singh, V. V., Rai, P. K. et Singh, D. 2015. Validation des marqueurs mole´culaires en vue du pyramidage assiste´par marqueurs des locus de re´sistance a`la rouille blanche chez la moutarde brune (Brassica juncea L.). Can. J. Plant Sci. 95: 939Á945. Pour eˆtre efficace, l'usage de marqueurs mole´culaires dans le pyramidage assiste´par marqueurs exige qu'on e´tablisse correctement le phe´notype de la cible. É valuer l'utilite´des marqueurs pour la se´lection assiste´e par marqueurs en proce´dant a`une validation croise´e dans divers contextes ge´ne´tiques et sur diffe´rentes populations est une e´tape cruciale de ce processus. Dans la pre´sente e´tude, les marqueurs At5g41560 et At2g36360 d'un intron polymorphe issu d'Arabidopsis, identifie´s ante´rieurement et pre´sentant un lien e´troit avec les marqueurs AcB1-A4

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Peronospora-like fungi (Chromista, Peronosporales) parasitic on Brassicaceae and related hosts

Cited by 121 publications

References 0 publications

Differential Recognition of Highly Divergent Downy Mildew Avirulence Gene Alleles by RPP1 Resistance Genes from Two Arabidopsis Lines

Differential Recognition of Highly Divergent Downy Mildew Avirulence Gene Alleles by RPP1 Resistance Genes from Two Arabidopsis Lines

Genetics of disease resistance in Arabidopsis to crop pathogens

Validation of molecular markers for marker-assisted pyramiding of white rust resistance loci in Indian Mustard (Brassica juncea L.)

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