Peroxidase activity against guaiacol, NADH, chlorogenic acid, ferulic acid and coniferyl alcohol in root tips of Lotus japonicus and L. corniculatus grown under low pH and aluminium stress

Zelinová, Veronika; Mistrı́k, Igor; Paľove-Balang, Peter; Tamás, Ladislav

doi:10.2478/s11756-010-0029-3

Cited by 11 publications

(6 citation statements)

References 42 publications

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“…Authors concluded that peroxidases act as compensation mechanisms which participate in detoxication of free radicals accumulated after gamma irradiation of seeds. Besides the antioxidant activity peroxidases are also involved in cell expansion influencing cell-wall strengthening (Zelinová et al 2010). More detailed picture about distribution of G-POX isoenzymes isolated from roots grown from LTP treated maize seeds are presented in Fig.…”

Section: Activity Of Antioxidant Enzymesmentioning

confidence: 99%

“…These ROS are detoxified by the sequential and simultaneous action of a number of enzymes, including superoxide dismutase (SOD), peroxidase (POX), and catalase (CAT). It's generally known, that the coordinated action of several antioxidant enzymes is activated when plants are exposed to different kinds of stress (Cakmak & Horst 1991;Suzuki & Mittler 2006;Agarwal 2007;Apostolova et al 2008;Zelinová et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

et al. 2012

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Abstract:The seeds of Zea mays L. cv. KWS were exposed to low-temperature plasma (LTP) by using Diffuse Coplanar Surface Barrier Discharge (DCSBD) for 60 and 120 seconds respectively. Growth parameters, anatomy of roots and activity of some enzymes (CAT, G-POX, SOD and DHO) isolated from roots grown from the seeds treated by LTP were evaluated. Our results indicate that LTP treatment of maize seeds affects post-germination growth of seedlings and this effect depends on the duration of LTP treatment. LTP treatment in duration of 60 seconds significantly increased the length, fresh and dry weight of the roots. However, the increase in time of LTP treatment to 120 seconds had inhibitive effect on the studied growth parameters. The activities of all studied antioxidant enzymes significantly increased with the age of maize seedlings in control conditions. On the other hand the application of LTP resulted in small, mostly non significant changes in the activity of antioxidant enzymes. Significant decrease in CAT activity was observed both in 3 and 6-day old maize roots and G-POX activity in 3-day old maize roots grown from seeds exposed to LTP for 60 seconds. A small, significant increase was detected only in SOD activity in 3-day old maize roots grown from seeds treated with LTP for 120 seconds and in 6-day old maize roots treated with LTP for 60 seconds. Significantly higher DHO activity was determined in embryos isolated from seeds treated with LTP for 60 seconds. On the contrary, in roots the DHO activity decreased with the time of LTP treatment. LTP treatment of seeds did not affect the anatomy of maize roots and caused only minor changes in the isoenzyme composition of G-POX and SOD.

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Section: Activity Of Antioxidant Enzymesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

et al. 2012

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“…After through washing, the fresh roots and leaves, tissues were crushed in 0.1 % trichloroacetic acid 61 with the aid of mortar and pestle and centrifuged for 15 min at 10,000 rpm. The supernatant was then mixed with potassium iodide (1M) and phosphate buffer (10 mM, ph 7.0) and kept for 1 h in the dark.…”

Section: Analysis H 2 O 2 In Plant Tissuesmentioning

confidence: 99%

The effect of low-pressure dielectric barrier discharge (LPDBD) plasma in boosting germination, growth, and nutritional properties in wheat

Hasan

Sohan

Sajib

et al. 2021

Preprint

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Plasma agriculture is an emerging technology, although the application of non-thermal plasma in wheat productivity is still in its early stage. This study deciphers the effect and mechanistic basis of non-thermal air-generated LPDBD (low-pressure dielectric barrier discharge) plasma in boosting germination, growth and nutritional properties in wheat. Seeds treated with LPDBD plasma exhibited cracked periphery and 6 min treatment showed a 22.11% increase in the germination rate compared to non-treated controls. At the cellular level, the concentration of H2O2 in leaves significantly increased (3.56 µM g-1FW) due to LPDBD plasma treatment, may act as a stimulating agent to trigger the physiological functions in wheat plants. In addition, plants sprouted from air-treated seeds exhibited a marked elevation in CAT and SOD activity accompanied by the increased expression of TaCAT and TaSOD genes in roots of wheat. Interestingly, the grain yield of wheat increased by 27.06% in response to plasma treatment compared to control. Further, grains harvested from plasma-treated plants showed a substantial elevation in iron and fat content as well as decreased moisture content that may contribute to the increased shelf life. The study will open up a new avenue for practical application of plasma in agriculture.

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“…The content of free protein was determined using the Coomassie brilliant blue method (Bradford, 1976), and the activities of POD and SOD enzymes were determined using the guaiacol (Zelinová et al, 2010) and NBT (Rukmini et al, 2004) methods, respectively. The contents of free sugars, amino acids, and polyphenols were measured using the anthrone (Li and Li, 2013), the ninhydrin (Sun et al, 2006), and the Folin phenol (Li et al, 2008) methods, respectively.…”

Section: Determination Of Physiological Indexes Related To Stress Resistancementioning

confidence: 99%

Metabolite Profile of Xylem Sap in Cotton Seedlings Is Changed by K Deficiency

et al. 2020

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Xylem sap, belonging to the plant apoplast, not only provides plant tissues with inorganic and organic substances but also facilitates communication between the roots and the leaves and coordinates their development. This study investigated the effects of potassium (K) deficiency on the morphology and the physiology of cotton seedlings as well as pH, mineral nutrient contents, and metabolites of xylem sap. In particular, we compared changes in root–shoot communication under low K (LK) and normal K (NK, control) levels. Compared to control, LK stress significantly decreased seedling biomass (leaf, stem, and root dry weight; stem and root length; root surface area and root volume) and the levels of K, Na (sodium), Mg (magnesium), Fe (iron), and Zn (zinc) in xylem sap. A total of 82 metabolites in sap analyzed by high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) showed significant differences between the two conditions; among these, 38 were up-regulated more than 2-fold, while the others were down-regulated less than 0.5-fold. In particular, several metabolites found in the cell membrane including three cholines (glycerophosphatecholine, 2-hexenylcholine, and caproylcholine) and desglucocoroloside and others such as malondialdehyde, α-amino acids and derivatives, sucrose, and sugar alcohol significantly increased under LK stress, indicating that cell membranes were damaged and protein metabolism was abnormal. It is worth noting that glycerophosphocholine was up-regulated 29-fold under LK stress, indicating that it can be used as an important signal of root–shoot communication. Furthermore, in pathway analyses, 26 metabolites were matched to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways; L-aspartic acid, which was associated with 10 KEGG pathways, was the most involved metabolite. Overall, K deficiency reduced the antioxidant capacity of cotton seedlings and led to a metabolic disorder including elevated levels of primary metabolites and inhibited production of secondary metabolites. This eventually resulted in decreased biomass of cotton seedlings under LK stress. This study lays a solid foundation for further research on targeted metabolites and signal substances in the xylem sap of cotton plants exposed to K deficiency.

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Peroxidase activity against guaiacol, NADH, chlorogenic acid, ferulic acid and coniferyl alcohol in root tips of Lotus japonicus and L. corniculatus grown under low pH and aluminium stress

Cited by 11 publications

References 42 publications

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

The effect of low-pressure dielectric barrier discharge (LPDBD) plasma in boosting germination, growth, and nutritional properties in wheat

Metabolite Profile of Xylem Sap in Cotton Seedlings Is Changed by K Deficiency

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