Peroxidase Activity and Cell Differentiation in Developing Salivary Glands of the Rats

Moriguchi, Keiichi; Yamamoto, Mitsuji; Asano, Taimei; Shibata, Takashi

doi:10.2535/ofaj1936.72.1_13

Cited by 19 publications

(23 citation statements)

References 28 publications

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“…The remaining known sublingual secretory proteins are products of the serous demilunes. All known serous demilune-specific proteins-SMGB, PSP, and CSP-1 (as well as prolactin-inducible protein (PIP) (Mirels et al, 1998a) and peroxidase (Moriguchi et al, 1995;Redman et al, 1998), which were not examined in the current study)-are also products of the immature acinar cells of the developing parotid and submandibular glands (Strum, 1971;Yamashina and Barka, 1972;Redman and Field, 1993;Mirels et al, 1998a).…”

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confidence: 78%

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Development of the rat sublingual gland: A light and electron microscopic immunocytochemical study

et al. 2001

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Cell differentiation in the rat sublingual gland occurs rapidly and is largely complete by birth. To study differentiation of the serous and mucous cells of the sublingual gland, we used antibodies to the secretory proteins CSP-1, SMGB, PSP, and SMGD, and sublingual mucin as specific cell markers. Glands from rats at ages 18, 19, and 20 days in utero, and postnatal days 0, 1, 5, 9, 14, 18, 25, 40, and 60 were fixed and prepared for morphological analysis and immunocytochemical labeling. At age 18 days in utero, a few cells in the developing terminal bulbs contained mucous-like apical granules that labeled with anti-mucin. Other cells had mixed granules with a peripheral lucent region and a dense core of variable size that occasionally labeled with anti-SMGD. Additionally, presumptive serous cells with small dense granules that contained CSP-1 and SMGB were present. At age 19 days in utero, the dense granules of these cells also labeled with anti-SMGD. By age 20 days in utero, mucous cells were filled with large, pale granules that labeled with anti-mucin, and serous cells had numerous dense granules containing CSP-1, SMGB, PSP, and SMGD. Fewer cells with mixed granules were seen, but dense regions present in some mucous granules (MGs) labeled with anti-SMGD. After birth, fewer MGs had dense regions, and serous cells were organized into well-formed demilunes. Except for PSP, which was undetectable after the fifth postnatal day, the pattern of immunoreactivity observed in glands of neonatal and adult animals was similar to that seen by age 20 days in utero. These results suggest that mucous and serous cells have separate developmental origins, mucous cells differentiate earlier than serous cells, and cells with mixed granules may become mucous cells. Anat Rec 266: 30 -42, 2002.

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confidence: 78%

“…Sublingual gland development has been investigated previously (Laj et al, 1971;Redman and Ball, 1978;Moriguchi et al, 1995;Taga and Sesso, 1998). The rat sublingual rudiment is initiated at age 14 days in utero, in the same mesenchymal capsule as the submandibular gland (Redman and Sreebny, 1970).…”

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confidence: 99%

Development of the rat sublingual gland: A light and electron microscopic immunocytochemical study

et al. 2001

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“…In Sprague-Dawley rats, natural weaning is known to start between 2 and 4 weeks of age, and it is during this period that the teeth of the animals begin to erupt 12) . Hamster submandibular gland seems to follow a similar developmental pattern, as mentioned above.…”

Section: Discussionmentioning

confidence: 99%

“…Five hamsters were used in each group. Under deep anesthesia with an intraperitoneal injection of sodium pentobarbital (50 mg/kg body weight), they were killed at birth and at 3,6,8,12,14,28 and 35 days, and at 3 and 6 months of age. The experimental protocol of this study followed the Guidelines for Animal Experiments at the School of Dentistry, Aichi-Gakuin University.…”

Section: Methodsmentioning

confidence: 99%

“…Then 40 μm thick sections were prepared by means of a Leica Cryocut 1800, and these sections were rinsed and kept overnight in cold 0.1 M PBS. The sections were then incubated in the reaction medium, a slight modification of Graham and Karnovsky 11) , consisting of 0.1% 3,3'-diaminobenzidinetetrahydrochloride (DAB, Dojindo Pharmaceutical Chemical Lab., Kumamoto, Japan) and 0.01% H 2 O 2 in PBS 12) . The incubations were carried out for 1 h at 37°C, followed by post fixation for 1 h at 4°C in 1% osmium tetroxide in PBS, and dehydration in graded ethanol solutions and embedding in Quetol 653 resin (Nisshin EM Co. Ltd., Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

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Light and Electron Microscopic Histochemistry of the Peroxidase Activity in the Secretory Granules of the Developing Hamster Submandibular Gland

Utsumi

Moriguchi

Ohno

2011

Okajimas Folia Anat. Jpn.

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Summary: On the basis of light and electron microscopic observations of the post natal development of the hamster submandibular gland, granules in the acinar cells showed considerably variations in size and shape, as well as electron density of the peroxidase-positive reaction. The present study shows that secretory granules of the hamster submandibular gland undergo changes of area and of intensity for peroxidase activity 6 months after birth.

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Secretory protein expression patterns during rat parotid gland development

et al. 1998

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The rat parotid gland produces a number of well-characterized secretory proteins. Relatively little is known, however, about the onset of their synthesis and cellular localization during gland development. Secretory protein expression was studied in parotid glands of fetal and postnatal rats using light and electron microscopic immunocytochemistry and Northern blotting. Amylase, parotid secretory protein (PSP), common salivary protein-1 (CSP-1), and SMGB were first detected by immunofluorescence in parotid glands of 18 day fetuses. By 5 days after birth, light and electron microscopic immunolabeling localized all of these proteins to the secretory granules of developing acinar cells. Labeling of acinar cells for DNAse I, however, was not observed until 18 days after birth. Between 9 and 25 days, CSP-1 and SMGB reactivity of acinar cells declined, but increased in intercalated duct cells. After 25 days, CSP-1 and SMGB were found only in intercalated ducts, and amylase, PSP, and DNAse I were restricted to acinar cells. Levels of CSP-1 and SMGB mRNA were relatively constant through 21 postnatal days, but declined significantly after that. Amylase and PSP mRNA increased rapidly and continuously from five days after birth to the adult stage. In contrast, DNAse I mRNA was not detectable until 18 days after birth. The immunocytochemical and molecular analyses define three basic patterns of protein expression in the rat parotid gland: proteins whose synthesis is initiated early in development and is maintained in the acinar cells, such as amylase and PSP; proteins that are initially synthesized by immature acinar cells but are restricted to intercalated ducts in the adult gland, such as CSP-1 and SMGB; and proteins that are synthesized only by mature acinar cells and first appear during the third postnatal week, such as DNAse I. The parotid gland exhibits four distinct developmental stages: prenatal, from initiation of the gland rudiment until birth; neonatal, from 1 day up to about 9 days postnatal; transitional, from 9 days to 25 days of age; and adult, from 25 days on. Although differences exist in timing and in the specific proteins expressed, these developmental stages are similar to those seen in the rat submandibular gland. Additionally, the results support the suggestion that intercalated ducts may differentiate from the neonatal acini.

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Peroxidase Activity and Cell Differentiation in Developing Salivary Glands of the Rats

Cited by 19 publications

References 28 publications

Development of the rat sublingual gland: A light and electron microscopic immunocytochemical study

Development of the rat sublingual gland: A light and electron microscopic immunocytochemical study

Light and Electron Microscopic Histochemistry of the Peroxidase Activity in the Secretory Granules of the Developing Hamster Submandibular Gland

Secretory protein expression patterns during rat parotid gland development

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