Peroxidase activity and endogenous free auxin during adventitious root formation

Gaspar, Th.; Kevers, C.; Hausman, Jean-François; Ripetti, V.

doi:10.1007/978-94-011-0790-7_32

Cited by 53 publications

(34 citation statements)

References 48 publications

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“…In our work the pattern of change in peroxidase activity was similar in the stem bases and leaves of IBAleaf bud cuttings of elepidote rhododendron. We found typical changes of peroxidase activity in the induction and initiation phases, in accord with results previously described (Gaspar et al, 1994). However, our results suggest that not only reduction but also a gradual increase of peroxidase activity can be used to define the expression phase.…”

Section: Discussionsupporting

confidence: 92%

“…Peroxidase activity has been used to define rooting phases (induction, initiation and expression) (Gaspar et al, 1994) in several species: Elaeis guineensis (Rival et al, 1997), Psoralea corylifolia (Rout et al, 2000), Arbutus unedo and Taxus baccata (Metaxas et al, 2004), Ebenus cretica , Gardenia jasminoides (Hatzilazarou et al, 2006) and Malus rootstock MM 106 (Naija et al, 2008). In our work the pattern of change in peroxidase activity was similar in the stem bases and leaves of IBAleaf bud cuttings of elepidote rhododendron.…”

Section: Discussionsupporting

confidence: 68%

“…A period of high peroxidase activity and cell dedifferentiation followed by cell division are characteristic of the initiation phase. In the expression phase there is a gradual drop in peroxidase activity accompanying the organization and growth of root primordia (Gaspar et al, 1994). Such changes of peroxidase activity have been reported also in Rhododendron catawbiense Michx.…”

Section: Introductionmentioning

confidence: 53%

“…Grönroos and Von Arnold (1987) reported that superoptimal IBA treatments extended the period for development of meristemoids and roots in hypocotyl cuttings of Pinus contorta in vitro, and the maximum number of roots was obtained more than a month after cutting. There is an inverse relationship between changes in auxin level and changes in specific peroxidase activity (Gaspar et al, 1994). High peroxidase activity in cuttings of the non-rooting genotype of E. cretica probably reduced the auxin level in tissues, which was a prerequisite for root formation to occur [soluble peroxidase activity was measured using 3-methyl-2-benzothiazolinone hydrazone (MBTH) and 3-(dimethylamino) benzoic acid (DMAB)] .…”

Section: Discussionmentioning

confidence: 99%

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Can Changes in Starch Content and Peroxidase Activity Be Used as Rooting Phase Markers for Rhododendron Leaf Bud Cuttings?

Megre¹,

Dokane²,

Kondratovics³

2011

Acta Biologica Cracoviensia Series Botanica

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We examined whether peroxidase activity in cutting bases and leaves and starch content in cutting bases can be used as rooting phase markers in the elepidote rhododendron cv. 'Babites Baltais' (Rhododendron L.). Changes in peroxidase activity in cutting leaves and bases, as well as starch content in cutting bases, were determined in relation to anatomical stages of rhizogenesis in leaf bud cuttings treated with 1% indole-3-butyric acid (IBA+) or without IBA (IBA-). The pattern of change of peroxidase activity was similar in cutting bases and leaves of IBAleaf bud cuttings. Three phases of adventitious root formation were identified: induction, initiation and expression. During the induction phase peroxidase activity decreased, but no anatomical changes were observed in the cuttings. Peroxidase activity increased in the initiation phase when adventitious root initials were formed. Peroxidase activity decreased during the expression phase when adventitious root primordia developed. The starch content of IBA-leaf bud cuttings decreased during the first few days and then gradually rose to maximum, followed by a sharp reduction and another increase at the end of the experiment. The changes of starch content did not coincide with rooting phases as peroxidase activity did, and cannot be used as a rooting phase marker in rhododendrons. Adventitious root formation did not occur in IBA+ leaf bud cuttings, so distinct rooting phases could not be observed. There was a significant correlation between peroxidase activity in cutting bases and leaves of IBA-leaf bud cuttings. Peroxidase activity in leaves of rhododendron leaf bud cuttings are potentially useful as a marker for rooting phases, but that requires further anatomical and physiological study of rooting in leaf bud cuttings.K Ke ey y w wo or rd ds s: : Peroxidase, starch, adventitious root, leaf bud cuttings, rhododendron.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 68%

Section: Introductionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Can Changes in Starch Content and Peroxidase Activity Be Used as Rooting Phase Markers for Rhododendron Leaf Bud Cuttings?

Megre¹,

Dokane²,

Kondratovics³

2011

Acta Biologica Cracoviensia Series Botanica

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“…In contrast, the level of POX activity in the hypocotyls without the ability to regenerate adventitious roots was high from the early days of culture. It is already known that POX activity regulates auxin catabolism and that the induction of rhizogenesis is characterized by a decrease in POX activity, while root development phase exhibits an increase in POD activity (Haissig and Davis 1994;Gaspar et al 1994). Since high endogenous auxin concentration is necessary for the induction phase of rhizogenesis, whereas decrease in auxin content is a crucial event during the roots formation and growth (de Klerk et al 1999), it can be stated that POX activity pattern during the course of adventitious roots formation from hypocotyls of M. crystallinum cultured on the media containing different anti-or prooxidants reflected the auxin level necessary to induce and proceed rhizogenesis.…”

Section: Specific Pattern Of Sod and Pox Activities Is Related With Tmentioning

confidence: 99%

Influence of anti- and prooxidants on rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured in vitro

Libik-Konieczny

Kozieradzka-Kiszkurno

Michalec-Warzecha

et al. 2017

Acta Physiol Plant

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The enrichment with antioxidants (glutathione or ascorbate) or prooxidants (alloxan, methylviologen, hydrogen peroxide) of root inducing medium significantly decreased rhizogenesis frequency (alloxan, hydrogen peroxide) or inhibited roots regeneration (ascorbate, methylviologen) during the in vitro culture of Mesembryanthemum crystallinum L. hypocotyls. The adventitious roots morphology, root hairs length and density, was also influenced. Changes in the rhizogenesis course were related to the differences in hydrogen peroxide concentration during following days of culture between explants exhibiting morphogenic potential and those without the ability to form adventitious roots. In explants with morphogenic potential, rhizogenesis induction was always accompanied by a high level of hydrogen peroxide followed by the decrease in H 2 O 2 content in following days. In contrast, in the explants without regeneration potential, the level of hydrogen peroxide was increasing during the culture period. Activity patterns of superoxide dismutase (SOD) and guaiacol peroxidase (POX) in the following days of culture were similar in the explants exhibiting regeneration potential cultured on different media. Total activity of SOD decreased during initial days of culture and then increased due to the activation of additional SOD isoform described as MnSODII. The activity of POX was low during the rhizogenesis induction, and then increased during following days of culture; the increase was correlated with the decrease in hydrogen peroxide content. In the explants without the ability to regenerate roots, the total activity of SOD was low throughout the whole culture period, whereas the POX activity was significantly higher than in hypocotyls with regeneration potential. It might be concluded that the increase in hydrogen peroxide during initial stages of rhizogenesis and the induction of MnSODII are prerequisites for adventitious roots formation from hypocotyls of M. crystallinum, independently in the presence of anti-or prooxidant in the culture medium.

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Comparative evaluation of oxidative enzyme activities during adventitious rooting in the cuttings of grapevine rootstocks

et al. 2011

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Peroxidase activity and endogenous free auxin during adventitious root formation

Cited by 53 publications

References 48 publications

Can Changes in Starch Content and Peroxidase Activity Be Used as Rooting Phase Markers for Rhododendron Leaf Bud Cuttings?

Can Changes in Starch Content and Peroxidase Activity Be Used as Rooting Phase Markers for Rhododendron Leaf Bud Cuttings?

Influence of anti- and prooxidants on rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured in vitro

Comparative evaluation of oxidative enzyme activities during adventitious rooting in the cuttings of grapevine rootstocks

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